A5100763425- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Developmental Biology and Gene Regulation
- Circadian rhythm and melatonin
- Light effects on plants
- Cell death mechanisms and regulation
- Plant Molecular Biology Research
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Trace Elements in Health
- Mobile Learning in Education
- Barrier Structure and Function Studies
- Medical Imaging and Pathology Studies
- Business Process Modeling and Analysis
- Gastric Cancer Management and Outcomes
- Augmented Reality Applications
- Chemokine receptors and signaling
- Evolutionary Algorithms and Applications
- RNA regulation and disease
- ATP Synthase and ATPases Research
- Salivary Gland Tumors Diagnosis and Treatment
- Extracellular vesicles in disease
- Vibrio bacteria research studies
- DNA Repair Mechanisms
Massey University
2022
University of Colorado Denver
2021
University of California, Irvine
2015-2021
Liaocheng People's Hospital
2020
City Of Hope National Medical Center
2014-2015
Harvey Mudd College
2013
Peterborough City Hospital
2012
National Institute of Allergy and Infectious Diseases
2011
National Institutes of Health
2011
Commonwealth Scientific and Industrial Research Organisation
2010
Fungi are the principal degraders of biomass in most terrestrial ecosystems. In contrast to surface environments, deep-sea environmental gene libraries have suggested that fungi rare and non-diverse high-pressure marine environments. Here, we report diversity from 11 samples around world representing depths 1,500 4,000 m (146-388 atm) two shallower water column (250 500m). We sequenced 239 clones 10 fungal-specific 18S rRNA constructed these samples, which detected only 18 fungal 18S-types...
Regulation of circadian clock components by phosphorylation plays essential roles in functions and is conserved from fungi to mammals. In the Neurospora negative feedback loop, FREQUENCY (FRQ) protein inhibits WHITE COLLAR (WC) complex activity recruiting casein kinases CKI CKII phosphorylate WC proteins, resulting repression frq transcription. On other hand, progressively FRQ promote degradation, a process that major determinant period length. Here, using whole-cell isotope labeling...
Protein phosphorylation plays essential roles in eukaryotic circadian clocks. Like PERIOD animals, the Neurospora core protein FRQ is progressively phosphorylated and becomes extensively before its degradation. In this study, by using purified from Neurospora, we identified 43 vivo sites mass spectrometry analysis. addition, show that CK-1a CKII are responsible for most events identify an additional 33 vitro kinase assays. Whole-cell metabolic isotope labeling quantitative MS analyses...
Endonuclease G (EndoG) is a nuclear-encoded mitochondrial protein reported to be important for both nuclear DNA fragmentation during apoptosis and replication. To evaluate the in vivo function of EndoG, we have investigated effects EndoG deficiency cells mice. We found that homozygous mutant embryos die between embryonic days 2.5 3.5. Mitochondrial copy numbers ovulated oocytes from heterozygous wild-type mice are similar, suggesting involved cellular unrelated Interestingly, exhibit...
To understand better the endogenous sources of MHC class I peptide ligands, we generated an antigenic reporter protein whose degradation is rapidly and reversibly controlled with Shield-1, a cell-permeant drug. Using this system, demonstrate that defective ribosomal products (DRiPs) represent major highly efficient source peptides are completely resistant to our attempts stabilize protein. Although also derive from nascent Shield-1-sensitive proteins "retirees" created by Shield-1...
Enhancers drive the gene expression patterns required for virtually every process in metazoans. We propose that enhancer length and transcription factor (TF) binding site composition-the number identity of TF sites-reflect complexity enhancer's regulatory task. In development, we define task as fates specified a set cells at once. hypothesize enhancers with more complex tasks will be longer, more, but less specific, sites. Larger numbers sites can arranged ways, allowing to many distinct...
We propose a service-oriented approach to generate and manage mashups. The proposed is realized using the Mashup Services System (MSS), novel platform support users create, use, mashups with little or no programming effort. relieves from programming-intensive, error-prone, largely nonreusable output process for creating maintaining describe overall design of MSS discuss evaluate its main enabling technologies.
This study investigates the attenuating effects of butyrate glycerides (BG) on intestinal inflammatory responses and barrier dysfunction induced by LPS stimulation. An initial dose-response test was carried out to identify optimal dose BG for further testing. The mice were given intragastric administration at different doses followed lipopolysaccharide (LPS) intraperitoneal injection. small morphology cytokine mRNA expression measured. With 1.5 g/kg BW administration, it possible alleviate...
ABSTRACT In Dictyostelium, initial cell type choice is correlated with the cell-cycle phase of at time starvation. We have isolated a mutant, ratioA (rtoA), defect in this mechanism that results an abnormally high percentage prestalk cells. The rtoA gene has been cloned and sequenced codes for novel protein. cycle normal rtoA. wild type, cells differentiate from those S or early G2 starvation prespore late M mutants, both originate randomly any
Abstract Background Non‐small cell lung cancer (NSCLC) occupies the majority of cases and is notorious for awful prognosis. LIM domains‐containing 1 (LIMD1) suggested as a tumor suppressor in cancer, but its mechanism NSCLC remains elusive. Present study aimed to uncover LIMD1 NSCLC. Methods qRT‐PCR was performed analyze level LIMD1. The functions cells were evaluated by CCK‐8, EdU, caspase‐3 activity assays. RIP pull‐down assays applied determine interaction with heterogeneous nuclear...
Abstract Antimicrobial copper‐containing surface materials have a great potential of reducing the risks healthcare‐associated infections (HAIs), but their increased use in hospital facilities may select copper‐resistant strains, causing concerns to antimicrobial resistance management. Here, we describe long‐term bacterial evolution experiment wherein non‐pathogenic Pseudomonas strain was subjected daily transfer laboratory media with and without copper‐mediated contact killing. The copper...
Abstract Proper development depends on precise spatiotemporal gene expression patterns. Most genes are regulated by multiple enhancers and often core promoters that generate similar transcripts. We hypothesize these may be required either because prefer a specific promoter or serve as redundancy mechanism. To test hypotheses, we studied the of knirps locus in early Drosophila melanogaster embryo, which is mediated promoters. found one resembles typical “sharp” developmental promoter, while...
Abstract Proper development depends on precise spatiotemporal gene expression patterns. Most developmental genes are regulated by multiple enhancers and often core promoters that generate similar transcripts. We hypothesize may be required either because prefer a specific promoter or serve as redundancy mechanism. To test these hypotheses, we studied the of knirps locus in early Drosophila melanogaster embryo, which is mediated promoters. found one resembles typical “sharp” promoter, while...
Ben Reichardt showed in a series of results that the general adversary bound function characterizes its quantum query complexity. This survey seeks to aggregate background and definitions necessary understand proof. Notable among these are lower proof, span programs, witness size, semi-definite programs. These definitions, addition examples detailed expositions, serve give reader better intuition graph-theoretic nature upper bound. We also include an applications this result bounds on...