A5101902526- Cancer therapeutics and mechanisms
- Light effects on plants
- Circadian rhythm and melatonin
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Cyclization and Aryne Chemistry
- Bioactive Compounds and Antitumor Agents
- Peptidase Inhibition and Analysis
- Photoreceptor and optogenetics research
- Cell death mechanisms and regulation
- Chronic Lymphocytic Leukemia Research
- Neurofibromatosis and Schwannoma Cases
- Drug Transport and Resistance Mechanisms
- Chemical Synthesis and Analysis
- Traditional Chinese Medicine Studies
- Biopolymer Synthesis and Applications
- 14-3-3 protein interactions
- Plant Disease Resistance and Genetics
- Marine Sponges and Natural Products
- Cancer Research and Treatments
- Bioactive natural compounds
- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
Guangxi University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Fujian Medical University
2023
China National Research Institute of Food and Fermentation
2018
Bioengineering Center
2015
National Center for Genetic Engineering and Biotechnology
2015
John Wiley & Sons (United States)
2015
Guangzhou University
2011
Southwestern Medical Center
2006-2007
Academy of Medical Sciences
2006-2007
Blue light regulates many physiological processes in fungi, but their photoreceptors are not known. In Neurospora crassa , all responses depend on the Per-Arnt-Sim (PAS) domain–containing transcription factor white collar–1 ( wc-1 ). By removing WC-1 light, oxygen, or voltage domain, a specialized PAS domain that binds flavin mononucleotide plant phototropins, we show abolished, including entrainment of circadian clock. However, WC-1–mediated dark activation frq remains normal this mutant,...
The eukaryotic circadian oscillators consist of autoregulatory negative-feedback loops. FRQ, WC-1, and WC-2 are three known components the loop Neurospora oscillator. FRQ represses its own transcription by interacting with WC-1/WC-2 complex inhibiting WC's role in transcriptional activation. Here we show that all associates FRH, an essential DEAD box-containing RNA helicase . budding yeast homolog Dob1p/Mtr4p, is a cofactor exosome, important regulator metabolism eukaryotes. Down-regulation...
The eukaryotic circadian oscillators consist of negative feedback loops. In Neurospora, it was proposed that the FREQUENCY (FRQ) protein promotes phosphorylation WHITE COLLAR (WC) complex, thus inhibiting its activity. kinase(s) involved in this process is not known. study, we show disruption interaction between FRQ and CK-1a (a casein kinase I homolog) results hypophosphorylation FRQ, WC-1, WC-2. ck-1a(L) strain, a knock-in mutant carries mutation equivalent to Drosophila dbt(L) mutation,...
Blue light regulates many molecular and physiological activities in a large number of organisms. In Neurospora crassa , eukaryotic model system for studying blue-light responses, the transcription factor photoreceptor WHITE COLLAR-1 (WC-1) its partner WC-2 are central to sensing. 's responses transient, that is, following an initial acute phase induction, light-regulated processes down-regulated under continuous illumination, phenomenon called photoadaptation. The mechanism(s)...
The COP9 signalosome (CSN) promotes the function of SCF-type cullin-based ubiquitin ligase complexes in vivo. Paradoxically, removal Nedd8 modification cullins by CSN inhibits activity SCF vitro. Ubiquitination-mediated degradation Neurospora circadian clock protein FREQUENCY (FRQ) is critical for function. Ubiquitination FRQ requires FWD-1, substrate-recruiting subunit an complex. Here we show that disruption a (csn-2) impairs and compromises its normal expression. A FRQ-independent...
In Neurospora, the flavin adenine dinucleotide-containing protein WHITE COLLAR-1 is blue-light photoreceptor for circadian clock and other light responses. The putative chromophore-binding domain of WC-1, its light, oxygen, or voltage (LOV) domain, similar to LOV domains found in plant phototropins, Neurospora VIVID (VVD) protein, Arabidopsis FKF1 related proteins. Studies phototropins have identified 11 flavin-contacting residues that are also conserved VVD, FKF1. this study, by mutating...
WHITE COLLAR-1 (WC-1) and WC-2, the two PAS domain-containing transcription factors, are positive elements of circadian feedback loops in Neurospora. In addition, both proteins essential components for light input various blue responses, including entrainment clock. Recently, we identified WC-1 as photoreceptor responsible these responses. this study, show that formation FRQ-WC complex vivo, a step critical closing negative loop, requires WC-1. negatively regulates expression ofwc-2 at level...
Regulation of circadian clock components by phosphorylation plays essential roles in functions and is conserved from fungi to mammals. In the Neurospora negative feedback loop, FREQUENCY (FRQ) protein inhibits WHITE COLLAR (WC) complex activity recruiting casein kinases CKI CKII phosphorylate WC proteins, resulting repression frq transcription. On other hand, progressively FRQ promote degradation, a process that major determinant period length. Here, using whole-cell isotope labeling...
Phosphorylation is a major regulatory mechanism controlling circadian clocks. In the Neurospora clock, PER-ARNT-SIM (PAS) domain-containing transcription factor, WHITE COLLAR (WC)-1, acts both as blue light photoreceptor of clock and positive element in negative feedback loop constant darkness, by activating frequency (frq) gene. To understand role WC-1 phosphorylation, five vivo phosphorylation sites, located immediately downstream zinc finger DNA binding domain, were identified tandem mass...
FREQUENCY (FRQ), a key component of the Neurospora circadian clock, is progressively phosphorylated after its synthesis. Previously, we identified casein kinase II (CKII) as that phosphorylates FRQ. Disruption catalytic subunit CKII abolishes clock function; it also causes severe defects in growth and development. To further establish role function, one regulatory genes, ckb1, was disrupted Neurospora. In ckb1 mutant strain, FRQ proteins are hypophosphorylated more stable than wild-type...
Berberine (BBR) has been suggested to be a hepatoprotective agent for oxidative‐stress‐related liver diseases because of its antioxidant activity. However, the mechanisms BBR are still not fully understood. In present study, protective effect was evaluated, and underlying molecular were investigated in hepatic cell line L02. Results from viability apoptosis assay showed that cells exposed hydrogen peroxide (H 2 O ), pretreatment 12 μM could increase by 19.10 ± 7.40% reduce apoptotic 7.91...
In this paper, 26 natural polymyxin components and a new derivative S2 were synthesized, their differences in efficacy toxicity have been investigated. Almost all of the synthesized showed strong activity against both susceptible resistant strains E. coli, K. pneumoniae, P. aeruginosa, A. baumannii. The toxicities obviously different between components. Only some tested for vivo. Compounds E2, E2-Val, A2, M2, D2, lower renal cytotoxicity acute than polymyxins B vivo nephrotoxicity was...
Dasatinib, a multikinase inhibitor, is used in the treatment of chronic myeloid leukemia and was developed to overcome imatinib resistance. Its mechanism action involves induction apoptosis, autophagy necroptosis. However, it remains unclear whether dasatinib can induce pyroptosis. In present study, gasdermin E (GSDME)-expressing SH-SY5Y A549 cells were chosen for investigation. Typical pyroptotic features, such as cleavage GSDME protein, leakage lactate dehydrogenase large bubbled...
Azidothymidine (zidovudine, AZT) used for treatment of HIV infection blocks the viral reverse transcriptase after phosphorylation by cellular enzymes. The first step in this reaction is formation AZT monophosphate, primarily catalyzed host cytoplasmatic thymidine kinase (TK1). activity TK1 was determined extracts PHA-stimulated peripheral blood mononuclear cells (PBMCs) from 20 healthy volunteers and 49 HIV-infected patients at different stages disease. In both groups we found a large intra-...
Twenty-three polymyxin analogs with variations at nine amino acid positions were synthesized and assessed for antimicrobial activity renal cytotoxicity. Compounds M2, 14, S2, 16 (MIC = 0.125–4 μg/mL) had similar or stronger activities against susceptible drug-resistant strains of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii compared to B 1–2 μg/mL). Most compounds (50% cytotoxic concentration, CC50 ≥ 200 exhibited lower cytotoxicity than (CC50 99 ±...
Oxidative stress is more likely to cause damage neuronal cells and mediates some neurodegenerative disorders. It well known that natural antioxidants can prevent oxidative become a potential therapeutic strategy. Noni juice obtained from the fruit of tree Morinda citrifolia, as folk medicine, has been used for over two thousand years. In current study, neuroprotective effect mechanism noni extracts against tert-Butyl hydroperoxide (TBHP)-induced SH-SY5Y cell were investigated. The results...
Bleomycin is a first-line clinically used antitumor antibiotic for effective treatment of certain types cancer in combination with other agents. Its action affected by bleomycin hydrolase (BLH), DNA repair enzymes, membrane transport proteins and cellular factors. To clarify whether BLH confers the resistance to tumor cells, it necessary further investigate roles its factors such as caveolin-1 bleomycin. In this study fourteen human cell lines were determination caveolin-1. The relationship...
In a recent study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996), 1H nuclear magnetic resonance (NMR) spectroscopy was used to demonstrate significant decreases in lactate levels after gamma irradiation of radiosensitive RIF-1 tumors vitro. For comparison, we have examined the effects radiation on more radioresistant EMT6 tumor. Single-slice (5-6 mm thick) localized spectra subcutaneous (untreated) and (pretreatment, 24 48 h postirradiation with 4, 10 or 20 Gy radiation) were...