A5024651958- Muscle Physiology and Disorders
- Animal Genetics and Reproduction
- RNA modifications and cancer
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Medicinal Plant Pharmacodynamics Research
- Immune Response and Inflammation
- Molecular Biology Techniques and Applications
- Traditional Chinese Medicine Analysis
- Land Use and Ecosystem Services
- Plant Reproductive Biology
- Soil Geostatistics and Mapping
- Neurogenetic and Muscular Disorders Research
- Adipose Tissue and Metabolism
- Cancer-related gene regulation
- Aquaculture disease management and microbiota
- Chromosomal and Genetic Variations
- Genetic and phenotypic traits in livestock
- Plant Molecular Biology Research
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
Zhongkai University of Agriculture and Engineering
2020-2025
Sichuan University
2024
University of Michigan
2024
Key Laboratory of Guangdong Province
2022-2023
Guangdong University of Education
2023
Shanxi Agricultural University
2021-2022
Hainan University
2022
Sun Yat-sen University
2014-2019
Chinese Academy of Medical Sciences & Peking Union Medical College
2014
Copy number variation (CNV) is an important type of genetic variations contributing to phenotypic differences in animals and may serve as alternative molecular marker single nucleotide polymorphism (SNP) for breeding. We used whole-genome sequencing data investigate the characteristic CNVs their associations with body weight size traits 504 120-day Lion-head geese. detected 1,184,695 which consisted 1,148,401 deletions 36,294 duplications. Based on these CNVs, we obtained 8,043 CNV regions...
Introduction The expansion of large-scale goose farming under semi-arid conditions has exacerbated bathing pool pollution, adversely affecting growth performance and intestinal health. Given the crucial role gut microbiota in maintaining homeostasis, considering reported beneficial effects bacteriophages Bacillus subtilis on health, this study investigated their combined application production. Methods To investigate phage supplementation a 90-day trial was conducted with 288 Magang goslings...
The hypothalamic-pituitary-gonad (HPG) axis is vital for reproductive activities in vertebrates. large-scale comparative analyses of gene expression the HPG across vertebrates have not been carried out yet. Here we collected 175 high-quality RNA-seq samples hypothalamus, pituitary, ovary and testis from eight species (four mammals four poultry) to compare transcriptome axis, detect key pathways related genes associated with reproduction. We demonstrated distinguished difference between...
Lipopolysaccharide (LPS) can induce systemic inflammation and affect the growth development of poultry. As a kind traditional Chinese medicine, polysaccharide Atractylodes macrocephala Koidz (PAMK) effectively improve performance animals immunity animal bodies.
Although the mechanism underlying modulation of transcription factors in myogenesis has been well elucidated, function cofactors involved this process remains poorly understood. Here, we identified HMGB2 as an essential nuclear transcriptional co-regulator myogenesis. was highly expressed undifferentiated myoblasts and regenerating muscle. Knockdown inhibited myoblast proliferation stimulated its differentiation. depletion downregulated Myf5 cyclin A2 at protein but not mRNA level. In...
Polysaccharide Of Atractylodes Macrocephala Koidz (PAMK) has been proved to have anti-cancer, antitumor, anti-inflammation function and improve the immune level of organism. The miRNA plays a very important role in regulating by negatively regulate expression target genes. To explore molecular mechanism PAMK active lymphocytes, thirty 61-d-old geese were randomly divided into 4 groups (C, CTX, PAMK, PAMK+CTX). thymus morphology, serum granulocyte-macrophage colony-stimulating factor...
Abstract Differentiation of myoblasts is essential in the development and regeneration skeletal muscles to form multinucleated, contractile muscle fibers. However, process myoblast differentiation mammals complicated requires be further investigated. In this study, we found Palmdelphin (Palmd), a cytosolic protein, promotes differentiation. Palmd predominantly expressed cytosol gradually up-regulated after Knockdown by small interfering RNA (siRNA) C2C12 markedly inhibits myogenic...
Kiwifruit ripening is a complex and highly coordinated process that occurs in conjunction with the formation of fruit edible quality. The significance epigenetic changes, particularly impact N6-methyladenosine (m6A) RNA modification on quality formation, has been largely overlooked. We monitored m6A levels gene expression changes kiwifruit at four different stages using LC-MS/MS, MeRIP, RNA-seq, validated function AcALKBH10 through heterologous transgenic tomato. Notable modifications...
Background The main challenge against patients with cancer to derive benefits from immune checkpoint inhibitors targeting PD-1/PD-L1 appears be the immunosuppressive tumor microenvironment (TME), in which IL-33/ST2 signal fulfills critical functions. However, whether IL-33 limits therapeutic efficacy of anti-PD-L1 remains uncertain. Methods Molecular mechanisms on treatment lewis lung carcinoma model were assessed by RNA-seq, ELISA, WB and immunofluorescence (IF). A sST2-Fc fusion protein...
Pig (Sus scrofa) is a major source of dietary proteins for human consumption and becoming valuable model in agricultural biomedical research. The recently developed isobaric tag relative absolute quantitation (iTRAQ) method allows sensitive accurate protein quantification. Here, we performed the first iTRAQ-based quantitative proteomic analyses Landrace (LR) Wuzhishan (WZS) pig longissimus dorsi muscle tissues during early embryonic development. study LR WZS ranging from 21 to 42 days post...
Zinc finger protein 422 (Zfp422) is a widely expressed zinc that serves as transcriptional factor to regulate downstream gene expression, but until now, little known about its roles in myogenesis. We found here Zfp422 plays critical role skeletal muscle development and regeneration. It highly expresses mouse during embryonic development. Specific knockout of impaired formation. Satellite cell-specific deletion severely inhibited Myoblast differentiation myotube formation were suppressed...
Abstract Objectives Mixed lineage leukaemia protein‐1 (MLL1) mediates histone 3 lysine 4 (H3K4) trimethylation (me3) and plays vital roles during early embryonic development hematopoiesis. In our previous study, we found its expression was positively correlated with myogenic ability in pigs, indicating potential mammalian muscle development. The present work aimed to explore the regulation mechanisms of MLL1 myogenesis. Materials methods C2C12 cells experimentally manipulated using small...
Skeletal muscle development from embryonic stages to hatching is critical for poultry growth, during which DNA methylation plays a vital role. However, it not yet clear how affects early between goose breeds of different body size. In this study, whole genome bisulfite sequencing (WGBS) was conducted on leg tissue Wuzong (WZE) and Shitou (STE) geese day 15 (E15), E23, post-hatch 1. It found that at the STE more intense than WZE. A negative correlation gene expression around transcription...
Soil organic matter (SOM), total nitrogen (TN), available phosphorus (AP), and potassium (AK) are important indicators of soil fertility when undertaking a quality evaluation. Obtaining high-precision spatial distribution map nutrients is great significance for the differentiated management nutrient resources reducing non-point source pollution. However, heterogeneity lead to uncertainty in modeling process. To determine best interpolation method, terrain, climate, vegetation factors were...
Pig is one of the major dietary protein sources for human consumption, from which muscle largest origin. However, molecular mechanisms concerning early porcine embryonic development distinctions between pig breeds are still unclear. In this study, an integrated analysis transcriptome and miRNAome was conducted using longissimus dorsi 4 stages around primary myofiber formation time (18-, 21-, 28-, 35-d post coitus) 2 (Landrace [LR] Wuzhishan [WZS]) differing in meat mass. The global...
Here, we performed whole-genome bisulfite sequencing of longissimus dorsi muscle from Landrace and Wuzhishan (WZS) miniature pigs during 18, 21, 28 d postcoitum. It was uncovered that in regulatory regions only around transcription start sites (TSSs), gene expression methylation showed negative correlation, whereas bodies, positive correlation occurred. Furthermore, earlier myogenic demethylation TSSs hypermethylation genes bodies were considered to trigger their pigs. by analyzing the...
CEP2 (CDC42EP2) is a member of the CDC42 subfamily that belongs to Rho family.The family plays an important role in variety cellular processes including skeletal myogenesis.Here, we find expression increased significantly during C2C12 myogenesis.Overexpression could attenuate myoblast differentiation, while knockdown by siRNA results enhancing myogenesis.Furthermore, demonstrate for first time attenuates differentiation via suppression muscle regulatory factors (MRFs) rather than influencing...
Fat-related traits have great influences on pork quality. As different fat tissues biochemical profiles depending their location, intramuscular contributes to gustatory qualities, while subcutaneous is considered as a negative factor associated with growth performance. In this study, both primary and vascular stem cells (IVSCs SVSCs) could be differentiated into mature adipocytes, though the IVSC differentiation efficiency was lower. By comparative analysis of transcriptomes, 2524...
In avian muscle development, embryonic development determines the number of myofibers after birth. Therefore, in this study, we investigated phenotypic differences and molecular mechanism pectoral European meat pigeon Mimas strain (later called pigeon) Shiqi on day 6 (E6), 10 (E10), 14 (E14) 1 birth (P1). The results showed that myofiber density was significantly higher than E6, with a diameter range 50~100 μm P1 were those pigeon. A total 204 differential expressed genes (DEGs) obtained...
Pigs supply vital dietary proteins for human consumption, and their economic value depends largely on muscle production. MicroRNAs are known to play important roles in skeletal development. However, relationship distinct production between pig breeds remains unknown. Here, we performed an integrated analysis of microRNA-mRNA expression profiles Landrace (LR, lean) pigs the Chinese indigenous Lantang (LT, lard-type) during 8 stages developmental, including at 35, 49, 63, 77 dpc (days post...