A5027312939- Circadian rhythm and melatonin
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Spaceflight effects on biology
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Circular RNAs in diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Dietary Effects on Health
- Light effects on plants
- TGF-β signaling in diseases
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Kruppel-like factors research
- Ubiquitin and proteasome pathways
- Estrogen and related hormone effects
- RNA Research and Splicing
- Genetic and phenotypic traits in livestock
- Bone Metabolism and Diseases
- Heat shock proteins research
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Exercise and Physiological Responses
Northwest A&F University
2014-2024
Education Department of Heilongjiang Province
2022-2023
Agriculture and Forestry University
2020
Sun Yat-sen University
2014
China Agricultural University
2014
Kyushu University
2010-2013
Chinese Academy of Medical Sciences & Peking Union Medical College
2012
Intramuscular fat (IMF) content affects the tenderness, juiciness, and flavor of pork. An increasing number studies are focusing on functions microRNAs (miRs) during porcine intramuscular preadipocyte development. Previous have proved that miR-425-5p was enriched in skeletal muscles played important roles multiple physiological processes; however, its adipogenesis remain unclear. To explore role adipogenesis, agomir inhibitor were used to perform overexpression knockdown preadipocytes,...
The meat quality of local breed pigs is more tender and juicier than the imported varieties. important reason that intramuscular fat content high. Even through modest sequence conservation evolution, expression pattern function long noncoding RNAs (lncRNAs) seem to be conserved. In spite that, analysis lncRNAs associated with development remains unknown us in porcine. Here, we systematically investigated adipocytes Bamei lean Large White consider on development. We selected three piglets...
MicroRNAs (miRNAs) are crucial regulatory molecules for adipogenesis. They contribute to the controlling of proliferation and differentiation preadipocytes. Previous studies revealed an important role miR-429 in cell invasion, migration, apoptosis. Our previous work has shown that expression subcutaneous fat can be observed newly born (3-day-old) Rongchang piglets rather than their adult counterparts (180-day-old). This pattern suggests might functionally related postnatal However, we...
Oleic acid is an abundant free fatty present in livestock that are a negative energy-balance state, and it may have detrimental effects on female reproduction fertility. induces lipid accumulation bovine granulosa cells, which leads to foam cell-like morphology reduced steroidogenesis. However, why oleic increases but decreases steroidogenesis remains unclear. This study focused acid's type steroidogenesis.Oleic increased the concentration-dependent manner mainly triglyceride level decreased...
The present study was designed to assess the relationship between gap junctions and maturation of a clock system in rat granulosa cells stimulated by follicle-stimulating hormone (FSH). Immature mature were prepared puncturing ovaries diethylstilbestrol- equine chorionic gonadotropin (eCG)-treated mouse Period2 ( Per2)- dLuc reporter gene transgenic rats, respectively. Mature exposed dexamethasone (DXM) synchronization displayed several Per2-dLuc oscillations rhythmic expression genes....
Ovarian development is related to cell proliferation, differentiation, and apoptosis of granulosa cells luteal under the control various modulators, including follicle-stimulating hormone (FSH), luteinizing (LH), growth factors. In present study, expression clock genes regulation mechanism were analyzed in different ovarian types during differentiation apoptosis. The authors focused on circadian Per2 as a core gene for maintenance rhythms. By using real-time monitoring system promoter...
<title>Abstract</title> Diabetes and many other metabolism syndromes are closely related to obesity. To reveal the underlying mechanism of fat deposition, an increasing number studies focusing on functions miRNAs during adipocytes development. Previous have proved that miR-15a/b play important roles in multiple physiological processes; however, their adipogenesis remain unclear. this, we detected expression profiles porcine pre-adipocyte, found levels increased early stage adipocyte...
The process of differentiation from preadipocytes to adipocytes contributes adipose tissue expansion in obesity. Blocking adipogenesis may be conducive the etiology obesity-related diseases. BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane protein, which was identified as target β-catenin colorectal hepatocellular tumor cells. However, whether BAMBI affects by Wnt/β-catenin signaling remains explored. In this study, we distinguish an differentiation. We found that...
Piglets are characteristically cold intolerant and thus susceptible to high mortality. However, browning of white adipose tissue (WAT) can induce non-shivering thermogenesis as a potential strategy facilitate the animal's response cold. Whether exposure subcutaneous WAT (sWAT) in piglets similar manner it humans remains largely unknown. In this study, were exposed acute (4°C, 10 h) or chronic (8°C, 15 days), genes proteins uncoupling protein 1 (UCP1)-dependent independent thermogenesis,...
Abstract The parameters of sperm apoptosis and capacitation during liquid storage at 17°C can indicate the quality pig potential development early embryos. However, effect kojic acid (KA) on semen preservation its mechanism has not been fully understood. In this study, we discovered that adding KA to diluent improved antioxidant capacity mitochondria, maintained normal structure reduced apoptosis. Western blot analysis revealed prevented release Cytochrome c from mitochondria cytoplasm,...
The involvement of FSH and triiodothyronine (T 3 ) in circadian clocks was investigated using immature granulosa cells ovaries during the progress cell maturation. Granulosa were prepared from preantral follicles mouse Period2 ( Per2) -dLuc reporter gene transgenic rats injected subcutaneously with synthetic nonsteroidal estrogen diethylstilbestrol. Analysis cellular clock performed partly a serum-free culture system. Several bioluminescence oscillations Per2-dLuc promoter activity generated...
Bone morphogenetic protein 2 (BMP2) belongs to the transforming growth factor-β superfamily and plays a critical role in regulating ovarian follicle function. Currently, of BMP2 during cumulus expansion remains be determined. The aim this study was investigate effect on regulation pentraxin 3 (PTX3) expression (the major component expansion) underlying mechanisms human granulosa-lutein (hGL) cells. Both primary immortalized hGL cells were used as research models. Our results showed that...
Granulosa cells (GCs) proliferation and estradiol synthesis significantly affect follicular development. The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that low-yielding sows, indicating may be involved sow fertility. However, functions mechanisms on GCs are unclear. This study focuses terms effects synthesis.Our findings revealed promotes inhibits porcine GCs. MiR-214-3p can increase percentage S-phase cells, number EdU labeled positive cell viability....
The proliferation of porcine ovarian granulosa cells (GCs) is essential to follicular development and the ubiquitin-proteasome system necessary for maintaining cell cycle homeostasis. Previous studies found that deubiquitinase ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) regulates female reproduction, especially in development. However, mechanism by which UCHL1 GC remains unclear. overexpression promoted proliferation, knockdown had opposite effect. directly bound cyclin B1 (CCNB1),...