A5102750839- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Circular RNAs in diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Advanced Nanomaterials in Catalysis
- Exercise and Physiological Responses
- Bee Products Chemical Analysis
- Reproductive biology and impacts on aquatic species
- Carcinogens and Genotoxicity Assessment
- Nanoparticles: synthesis and applications
- Bone Metabolism and Diseases
- Air Quality and Health Impacts
- Neurobiology and Insect Physiology Research
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Quinazolinone synthesis and applications
- Growth Hormone and Insulin-like Growth Factors
- Meat and Animal Product Quality
- Vitamin D Research Studies
Northwest A&F University
2007-2024
Agriculture and Forestry University
2020-2021
The University of Tokyo
2013
Bibra International
1998
The physiological and beneficial actions of vitamin D in bone health have been experimentally clinically proven mammals. active form [1α,25(OH)(2)D(3)] binds activates its specific nuclear receptor, the receptor (VDR). Activated VDR prevents release calcium from storage to serum by stimulating intestinal absorption renal reabsorption. However, direct action tissue is poorly understood because Ca(2+) homeostasis maintained through tightly regulated ion transport kidney, intestine, bone. In...
The meat quality of local breed pigs is more tender and juicier than the imported varieties. important reason that intramuscular fat content high. Even through modest sequence conservation evolution, expression pattern function long noncoding RNAs (lncRNAs) seem to be conserved. In spite that, analysis lncRNAs associated with development remains unknown us in porcine. Here, we systematically investigated adipocytes Bamei lean Large White consider on development. We selected three piglets...
MicroRNAs (miRNAs) are crucial regulatory molecules for adipogenesis. They contribute to the controlling of proliferation and differentiation preadipocytes. Previous studies revealed an important role miR-429 in cell invasion, migration, apoptosis. Our previous work has shown that expression subcutaneous fat can be observed newly born (3-day-old) Rongchang piglets rather than their adult counterparts (180-day-old). This pattern suggests might functionally related postnatal However, we...
Differentiation from preadipocytes into mature adipocytes is a complex biological process in which miRNAs play an important role. Previous studies showed that miR-214-3p facilitates adipocyte differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) vitro. The detailed function and molecular mechanism development unclear. In this study, the 3T3-L1 cell line was used to analyze Using 5-Ethynyl-2'-deoxyuridine (EdU) staining CCK-8 assay, we observed transfection with agomir visibly...
Abstract MicroRNAs (miRNAs) have critical roles during adipogenesis; however, their precise functions are not completely understood. Porcine miRNA expression profiles show that miR‐127 is dramatically downregulated with age in adipose tissue. We aimed to identify the and mechanisms of proliferation adipogenesis. Preadipocytes were cultured under conditions induce or differentiation effect overexpression on these processes, associated bioinformatically predicted target genes, assessed using...
Abstract Objectives Intramuscular fat ( IMF ) has a significant influence on porcine meat quality. Ubiquitin D UBD is involved in the management of diverse intracellular processes. However, its physiological functions adipose cell differentiation and proliferation are still poorly defined. Materials methods subcutaneous preadipocytes were isolated from longissimus dorsi neck deposits Chinese native Guanzhong Black piglets (3‐5 days old), respectively. Lentivirus with short hairpin RNA (sh...
Chemical residues in the environment are considered to be important factors that cause obesity. Bifenthrin is one of pyrethroid pesticides and widely used worldwide. However, its effect on adipose tissue ill-defined. Here, we administered bifenthrin/corn oil adult C57BL/6 mice by gavage. After 6 weeks, bifenthrin treatment significantly increased their body weight (P = 0.015) fat mass < 0.001). Then identified 246 differently expressed proteins proteomic analysis, they were highly involved...
Abstract Myoblast proliferation and terminal differentiation are the key steps of myogenesis. MicroRNAs a class small noncoding RNAs that play important roles in gene expression regulation. They negatively regulate by causing messenger RNA translational repression or target degradation. Here, we found microRNA‐423‐5p (miR‐423‐5p) is highly expressed both slow fast muscles. Our gain‐of‐function study indicated miR‐423‐5p actually plays negative role regulating myoblast differentiation. We...
It is well-documented that CL316,243 (a β3 agonist) or rosiglitazone PPARγ can induce white adipocyte populations to brown-like adipocytes, thus increasing energy consumption and combating obesity. However, whether there a combined effect remains unknown. In the present study, stromal vascular cells of inguinal adipose tissue (iWAT-SVCs for short) from mice were cultured induced into browning by CL316,243, rosiglitazone, both. Results showed combination significantly upregulated expression...
Abstract MicroRNA (miRNA) is essential for the process of gene posttranscriptional regulation in skeletal muscle many species, such as mice, cattle and so on. However, a little number miRNAs have been reported development Chinese native pig breeds. In this study, longissimus dorsi transcripts Rongchang at weaning slaughter time points were analysed miRNA‐seq. The results showed that 19 novel 186 known involved identified. Based on these findings, we further confirmed porcine miR‐127, miR‐299...
The number and distribution of adipocytes directly affect the quality livestock meat products. analysis adipogenesis mechanism is basis for improving quality. formation regulated by many factors, including a class endogenous small RNAs, named microRNA (miRNA). Previous studies have shown that miRNAs could post-transcriptional regulation target genes. In our study, decreased miR-99b-5p expression level was found in adipose tissue obese mice. Overexpression increase cell proliferation...
Pig breeds have significant differences in fat deposition and muscle development ability. However, the molecular mechanism behind these is still unknown. In this study, expression patterns of three candidate genes, suppressor cytokine signaling 3 (SOCS-3), obesity (ob) insulin-like growth factor I (IGF-I), which are involved adipose metabolism or development, were analyzed. Total RNA was extracted from dorsal subcutaneous tissue longissimus 8-month-old Bamei Largewhite pigs. Semiquantitative...
Evodiamine is a botanical alkaloid compound extracted from Tetradium plants. Previous studies have reported that evodiamine (Evo) treatment can reduce food uptake and improve insulin resistance in animals . The skeletal muscle comprises about 40% of the body mass adults has vital role regulating whole glucose metabolism energy metabolism. However, effect Evo on unclear. main aim present study was to investigate differentiation proliferation mouse C2C12 cell line. results demonstrated...
Abstract Background: Artificial insemination technology is the production used in more than 99% of world's pig farms. Preservation semen mainly conducted at room temperature (17°C), during which accumulation reactive oxygen species (ROS). In our research, by adding different concentrations hydroxytyrosol to diluent storage boar 17°C, we compared them with traditional study cumulative amount ROS, extent membrane damage, sperm acrosome integrity, activity antioxidant enzymes, and other...
Abstract Titanium dioxide nanoparticles (TiO 2 NPs) are widely added to various types of foods as food additives. Previous studies have shown that TiO NPs exposure can cause abnormal deposition adipose tissue in organisms, resulting lipid metabolism disorder. However, the potential molecular mechanisms underlying NPs’ effects yet be elucidated. In this study, our data indicated (100 mg/Kg BW, 20 nm) accelerated fat epididymal tissues and disturbed level blood glucose subsequently normal-fat...
Abstract Background: Artificial insemination technology is the production used in more than 99% of world's pig farms. As frozen sperm damage very serious, preservation mainly carried out at 17°C. However, accumulation a large amount reactive oxygen species (ROS) during process main reason for deterioration quality. In our research, by adding different concentrations hydroxytyrosol to diluent storage 17°C, we compared them with traditional study cumulative ROS, extent membrane damage,...