A5101718042- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- RNA Interference and Gene Delivery
- Renal and related cancers
- Mesenchymal stem cell research
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genital Health and Disease
- Plant tissue culture and regeneration
- Clusterin in disease pathology
- Aquatic life and conservation
- MicroRNA in disease regulation
- Ovarian function and disorders
- Dermatology and Skin Diseases
- Erythrocyte Function and Pathophysiology
- Hormonal and reproductive studies
- Hair Growth and Disorders
- Genetic diversity and population structure
- Biopolymer Synthesis and Applications
- Insect Utilization and Effects
Northwest A&F University
2014-2024
Shanghai Institutes for Biological Sciences
2009
Chinese Academy of Sciences
2009
Center for Excellence in Molecular Cell Science
2009
Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect boar seminal exosomes function during long-term liquid storage. Boar had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such AWN, CD9 CD63 western blot analysis. The parameters adding different ratio to preparations was analyzed. Compared diluent without exosomes, with four times or sixteen compared original semen higher...
Chlorpyrifos (CPF) is the most frequently applied insecticide. Aside from effects on neuronal cholinergic system, previous studies suggested a potential relationship between CPF exposure and male infertility; however, molecular mechanism remains elusive. The aim of this study was to investigate toxic effect testicular cells via in vitro vivo experiments. cytotoxic mouse-derived spermatogonial cell lines (GC-1), Sertoli (TM4) Leydig (TM3) were assessed by CCK-8 assay, flow cytometry, TUNEL...
Self-renewal and differentiation of spermatogonial stem cells (SSCs) are the foundation spermatogenesis throughout a male’s life. SSC transplantation will be valuable solution for young male patients to preserve their fertility. As SSCs in collected testis tissue from very limited, it is necessary expansion vitro. Previous studies suggested that histone methyltransferase ERG-associated protein with SET domain (ESET) represses gene expression essential maintenance pool embryonic neurons. The...
Male germline stem cells (mGSCs) are present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived vitro and resemble embryonic (ESCs) properties both the mouse humans. However, little is know about these domestic animals. Here we report first successful establishment of goat GSCs from 2–5-month fetal testis, developmental potential assay vivo. These express pluripotent markers such as Oct4, Sox2, C-myc, Tert when cultured human...
Stem Leydig cells (SLCs), located in the testicular interstitial compartment mammalian testes, are capable of differentiating to testosterone-synthesizing (LCs), thus providing a new strategy for treating testosterone deficiency. However, no previous reports have identified and cultured SLCs derived from pig. The aim current study was isolate, identify, culture pigs. Haematoxylin eosin staining immunochemical analysis showed that were present PDGFR α mainly expressed pig interstitium,...
2-Bromopalmitate (2-BP) is a palmitoylation inhibitor that can prevent the binding of palmitic acid to proteins, thereby exhibiting significant effects in promoting inflammation and regulating immune system. However, limited research has been conducted regarding direct 2-BP on animal organism. Therefore, we probed mice injected with for altered expression inflammatory genes, focus demonstrating changes intestinal flora as well damage reproductive Our findings indicate induce substantial...
In the present study, trehalose was utilized to improve primary culture of mouse epididymal epithelial cells in vitro, and enhance naked DNA delivery epididymis vivo. During six-day culture, proliferation activity medium with addition higher than that those cultured absence (p<0.01). To determine optimal concentration for cell proliferation, a series concentrations (0, 60, 120, 180 mM) were tested, result indicated 120 mM showed highest potential. The containing upon 16th passage, they...
Epididymal specific proteins play a crucial role in sperm maturation. Some of the post-translational modified are transported from caput to cauda epididymis through exosomes which regulate function epididymis. Rat beta-galactosidase-1-like protein 4 (GLB1L4) expressed specifically epididymis, localizes on sperm; however, regulatory ways GLB1L4 interacts with maintain unclear. In this study, knockdown rat could inhibit vitro capacitation and reduce fertility male rats by injection special...
Abstract A suitable additive for fish sperm storage in vitro is necessary artificial reproduction. In this study, we evaluated the effects of different concentrations (100, 200, 400, and 800 µmol/L) metformin (Met) on Schizothorax prenanti Onychostoma macrolepis under 72 h. Compared with control group, 400 µmol/L Met was more effective at improving quality fertilization capacity S. by increasing adenosine triphosphate (ATP) content within sperm. Further study found that stabilized ATP level...
Sialic acid (SA), which usually occupies the terminal position of oligosaccharide chains in mammalian spermatozoa, has important functions fertilization. Compared with other methods, such as lectin probing, boronic could recognize and bind SA a higher affinity specificity at pH 6.9. In this study, two carriers, 3-aminophenylboronic acid-labeled fluorescent latex (CML-APBA) magnetic beads (CMM-APBA were applied to explore surface sialylation profile sialoglycoproteins boar sperm. There are...
The adult Leydig cells (ALCs), originated from stem (SLCs), can secrete testosterone which is essential for germ cell development and sexual behavior maintenance. As a synthetic compound, ethane dimethane sulphonate (EDS), well-known alkylating agent, has been reported to specifically ablate ALCs. In this study, EDS was verified differentiated pig LCs by experiments. Subsequently, the primary isolated (containing SLCs LCs) EDS-treated (almost exclusively SLCs) were collected RNA-seq. 4,904...
Abstract BackgroundSpermatogenesis is a highly complicated biological process that occurs in the epithelium of seminiferous tubules. It regulated by complex network endocrine and paracrine factors juxtacrine testicular cross-talk . Sertoli cells (SCs) play key role spermatogenesis due to their production trophic, differentiation immune-modulating factors. However, many molecular pathways SCs action remain controversial unclear. Recently, research has focused on exosomes as an important...
The Onychostoma macrolepis (only wild population) has been listed as a national class II key protected animal in China. Currently, there is no specific compound feed available for its artificial breeding. dietary amino acid pattern an important reference formulation. This study aimed to investigate effects of the four different patterns, i.e., anchovy fishmeal protein (FMP, control group) and muscle (MP), whole-body (WBP), fish egg (FEP) juveniles O. macrolepis, on growth performance, body...