A5101905921- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Thyroid Disorders and Treatments
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Birth, Development, and Health
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Renal and related cancers
- Developmental Biology and Gene Regulation
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Cancer-related gene regulation
- Phytochemical Studies and Bioactivities
- Management and Organizational Studies
- Thyroid Cancer Diagnosis and Treatment
- Lipoproteins and Cardiovascular Health
- Ginseng Biological Effects and Applications
- Natural product bioactivities and synthesis
- Metabolism, Diabetes, and Cancer
- Anesthesia and Neurotoxicity Research
- Job Satisfaction and Organizational Behavior
- Atherosclerosis and Cardiovascular Diseases
- Ginger and Zingiberaceae research
- Cell death mechanisms and regulation
Shanghai Jiao Tong University
2015-2025
Shanghai Ninth People's Hospital
2021-2025
Qilu Hospital of Shandong University
2012-2021
Shandong University
2014-2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2021
Yuhuangding Hospital
2018-2020
Qingdao University
2018-2020
Shanghai Institutes for Biological Sciences
2008-2018
Chinese Academy of Sciences
2009-2018
Shanghai Institute of Nutrition and Health
2018
Multiciliated cells (MCCs) possess multiple motile cilia and are distributed throughout the vertebrate body, performing important physiological functions by regulating fluid movement in intercellular space. Neither their function during organ development nor molecular mechanisms underlying multiciliogenesis well understood. Although dysregulation of members miR-34 family plays a key role progression various cancers, miR-34b, especially formation, is largely unknown. Here, we demonstrate that...
Endoplasmic reticulum–localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum–associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from ER lumen under conditions stress. To elucidate function ERdj4 in vivo, we disrupted locus using gene trap (GT) mutagenesis, leading hypomorphic expression mice homozygous for trapped allele (ERdj4 GT/GT ). Approximately half died perinatally associated...
Angiogenesis is the formation of new blood vessels from existing vasculature, which involved in multiple biological processes, including atherosclerosis, ischemic heart disease, and cancer. Ginsenoside-Rb1 (Rb1), most abundant ginsenoside isolated form Panax ginseng, has been identified as a promising anti-angiogenic agent via up-regulation PEDF. However, underlying molecular mechanisms still remains unknown. In present study, human umbilical vein endothelial cells (HUVECs) were selected to...
Abstract Objective To investigate whether the mRNA expression of thyroglobulin ( TG ) and TSH receptor TSHR in lymph node could be used to diagnose metastasis patients with papillary thyroid cancer (PTC). Subjects methods Around 156 paraffin samples nodes from 89 PTC after surgery were collected, expressions detected by nested qPCR. Results Compared results confirmed histopathology, 86 out 87 tissues ectopically expressed mRNA, while 66 69 non‐metastasis did not express mRNA. The specificity...
Endonuclease G (EndoG) is a nuclear-encoded mitochondrial protein reported to be important for both nuclear DNA fragmentation during apoptosis and replication. To evaluate the in vivo function of EndoG, we have investigated effects EndoG deficiency cells mice. We found that homozygous mutant embryos die between embryonic days 2.5 3.5. Mitochondrial copy numbers ovulated oocytes from heterozygous wild-type mice are similar, suggesting involved cellular unrelated Interestingly, exhibit...
Background Reduced expression of developmentally important genes and tumor suppressors due to haploinsufficiency or epigenetic suppression has been shown contribute the pathogenesis various malignancies. However, methodology that allows spatio-temporally knockdown gene in model organisms such as zebrafish not well established, which largely limits potential a vertebrate human malignant disorders. Principal Finding Here, we report multiple copies small hairpin RNA (shRNA) are expressed from...
The phenotypic switch of vascular smooth muscle cells (VSMCs) is a key event in the pathogenesis various diseases, such as atherosclerosis and post-angioplasty restenosis. Small non-coding microRNAs (miRNAs) have emerged critical modulators VSMC function. In present study, miR-26a was significantly increased cultured VSMCs stimulated by platelet-derived growth factor-BB (PDGF-BB) arteries with neointimal lesion formation. Moreover, we demonstrated that regulates expression differentiation...
Purpose: To investigated the effects of ginsenoside Rb1 on diabetic retinopathy in streptozotocin-induced rats. Methods: Diabetes was induced by a single intraperitoneal injection streptozotocin (80 mg/kg) male Wistar Ginsenoside (20, 40 injected (i.p.) once day for 4 weeks. Then, using fundus photography, diameter and vascular permeability retinal vessels were investigated. Retinal histopathology undertaken. Contents malondialdehyde (MDA) glutathione (GSH) retinas assayed. Levels nuclear...
Dysregulation of ribosome biogenesis causes human diseases, such as Diamond-Blackfan anemia, del (5q-) syndrome and bone marrow failure. However, the mechanisms blood disorders in these diseases remain elusive. Through genetic mapping, molecular cloning mechanism characterization zebrafish mutant cas002, we reveal a novel connection between ribosomal dysfunction excessive autophagy regulation hematopoietic stem/progenitor cells (HSPCs). cas002 carries recessive lethal mutation kri1l gene...
Congenital hypothyroidism (CH) is a common congenital endocrine disorder in humans. CH-related diseases such as athyreosis, thyroid ectopy, and hypoplasia are primarily caused by dysgenic development. However, the underlying molecular mechanisms remain unknown.To identify novel CH candidate genes, 192 patients were enrolled, target sequencing of 21 known genes was performed. The remaining 98 carrying no subjected to exome (ES). functions identified variants confirmed using epithelial cells...
Two new steroidal saponins, dioscoresides C (1) and D (2), along with a natural product, pregnadienolone 3-O-beta-gracillimatriose (3), two known compounds, 3-O-beta-chacotrioside (4) pseudoprotodioscin (5), were isolated from the rhizomes of Dioscorea panthaica Prain et Burkill. On basis extensive NMR studies chemical evidence, determined to be 26-O-beta-D-glucopyranosyl-3...
In vertebrate definitive hematopoiesis, nascent hematopoietic stem/progenitor cells (HSPCs) migrate to and reside in proliferative microenvironment for transitory expansion. this process, well-established DNA damage response pathways are vital resolve the replication stress, which is deleterious genome stability cell survival. However, detailed mechanism on repair of stress-induced during progenitor expansion remains elusive. Here we report that a novel zebrafish mutantcas003 with nonsense...
Loss of the tumor suppressor phosphatase and tensin homolog (PTEN) is frequently observed in hematopoietic malignancies. Although PTEN has been implicated maintaining quiescence stem cells (HSCs), its role hematopoiesis during ontogeny remains largely unexplored. The expression marker genes was analyzed via whole mount situ hybridization assay ptena ptenb double mutant zebrafish. embryonic myelopoiesis characterized by living imaging immunofluorescence with confocal microscopy, as well...