- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Microbial infections and disease research
University of Trás-os-Montes and Alto Douro
2023-2024
Instituto Nacional de Investigação Agrária e Veterinária
2022-2024
Abstract Staphylococcal enterotoxin A (SEA) is the most frequently reported in staphylococcal food poisoning (SFP) outbreaks. Aptamers are single-stranded nucleic acids that seen as promising alternatives to antibodies several areas, including diagnostics. In this work, systematic evolution of ligands by exponential enrichment (SELEX) was used select DNA aptamers against SEA. The SELEX protocol employed magnetic beads an immobilization matrix for target molecule and real-time quantitative...
Enterotoxigenic Escherichia coli (ETEC) is the major bacterial cause of diarrheal diseases in pigs, particularly at young ages, resulting significant costs to swine farming. The pathogenicity ETEC largely dependent on presence fimbriae and ability produce toxins. Fimbriae are responsible for their initial adhesion intestinal epithelial cells, leading onset infection. In particular, F4 type (K88) often attributed neonatal infections have also been associated with post-weaning infections. This...