Peripheral nerve injury can result in significant clinical complications that have uncertain prognoses. Currently, there is a lack of effective pharmacological interventions for damage, despite the existence several small compounds, peptides, hormones, and growth factors been suggested as potential enhancers neuron regeneration. Despite objective achieving full functional restoration by surgical intervention, persistent challenge inadequate recovery remains concern context peripheral injuries.

Traumatic brain injury (TBI) is characterized by a disruption in the normal function of due to an following trauma, which can potentially cause severe physical, cognitive, and emotional impairment. Stem cell transplantation has evolved as novel treatment modality management TBI, it potential arrest degeneration promote regeneration new cells brain. Wharton's Jelly-derived mesenchymal stem (WJ-MSCs) have recently shown beneficial effects functional recovery neurological deficits.

Cerebral palsy (CP) describes a group of disorders affecting movement, balance, and posture. Disturbances in motor functions constitute the main body CP symptoms. These symptoms surface early childhood patients are affected for rest their lives. Currently, treatment involves various pharmacotherapies different types CP, including antiepileptics epilepsy Botox A focal spasticity. However, none these methods can provide full symptom relief. This has prompted researchers to look new modalities,...

The aim of the present study was to investigate effects three different formulations hyaluronic acid (HA): Low molecular weight (MW) Sinovial One®, medium MW Viscoplus® and high Durolane®, on chondrocyte proliferation collagen type II (COL2A1), hypoxia-inducible factor 1α (HIF-1α) chondroadherin (CHAD) expression in primary cultures. Standard cultures were established from osteochondral tissues surgically obtained 6 patients with gonarthrosis. Cell morphology evaluated using an inverted...

Electron spin resonance (ESR) is a powerful technique to detect radicals trapped in cellulosic food products and has been suggested as useful method for identification of irradiated herbal foodstuffs. Coriander spice which important medicinal properties was investigated using ESR spectroscopy. Radicals natural coriander samples were determined at room temperature. spectra sample characterized by single central signal with value gamma irradiation produced satellite peaks attributed...

Non-steroidal anti-inflammatory drugs (NSAIDs) that are applied through oral, injectable or topical routes have been widely used in painful and inflammatory musculoskeletal diseases. The current study aimed to determine whether naproxen, an aryl acetic acid derivative with analgesic effects, has a toxic effect on human chondrocytes. Samples containing monolayer primary chondrocyte cultures were prepared following resection from osteochondral tissues obtained patients gonarthrosis. Cell...

In the literature, there have been no studies showing clear results on how radio-contrast pharmaceuticals would affect intact disc tissue cells. this context, it was aimed to evaluate effects of iopromide and gadoxetic acid, frequently used in discography, lumbar pharmaco-molecular histopathological level. Primary cell cultures were prepared from healthy patients operated neurosurgery clinic. Except for control group, incubated with indicated agents. Cell viability, toxicity proliferation...

The aim of the present study was to determine whether pharmaceutical preparations with pregabalin (PGB) as an active ingredient, which are widely prescribed by clinicians, exert toxic effects on human primary nucleus pulposus (NP) and annulus fibrosis (AF). Primary cell cultures were obtained from intact (n=6) degenerated tissues resected two groups patients. Different doses PGB applied these cells subjected molecular analyses at 0, 24 48 h. Cell vitality, toxicity proliferation assessed...

The study aimed to investigate the effects of active ingredient, nimodipine, on chondrocyte proliferation and extracellular matrix (ECM) structures in cartilage tissue cells.

Hypoxic-ischemic encephalopathy (HIE) is one of the leading causes death and long-term neurological impairment in pediatric population.Despite a limited number treatments to cure HIE, stem cell therapies appear be potential treatment option for brain injury resulting from HIE. AIMTo investigate efficacy safety cell-based patients with METHODSThe study inclusion criteria were determined as presence substantial deficit disability caused by HIE.Wharton's jelly-derived mesenchymal cells...

AIM:To design a novel, polyvinyl alcohol (PVA)-based polymeric scaffold that permits the controlled release of insulin-like growth factor 1 (IGF-1)/bone morphogenetic protein (BMP)-2 following intervertebral disc administration. MATERIAL and METHODS:The drug delivery system was composed two different solutions formed within seconds coming into contact with each other.Swelling, pH, temperature tests analysis factors (GFs) from this were performed.The kinetics GFs determined through...

The aim of the present study was to investigate effects etanercept (ETA), a tumor necrosis factor (TNF) inhibitor, on human cell cultures prepared from intact intervertebral disc tissue. ETA is used as treatment for cases rheumatoid arthritis, psoriatic axial spondyloarthritis and ankylosing spondylitis accompanied by moderate or severe joint pain. applied primary [annulus fibrosus nucleus pulposus (NP) tissue]. Cell without served control group. Morphological quantitative molecular analyses...

To determine the gene expression patterns of nucleus pulposus (NP) in cell cultures obtained from degenerated or intact tissues.Whereas 12 cases were diagnosed with lumbar disc herniation and had undergone microdiscectomy, traumatic intervertebral discectomy corpectomy, along after spinal trauma. NP-specific markers expressions reagents extracellular matrix experimental setup tested at 0th, 24th, 48th hours by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Visual...

The present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti-pyretic and anti-spasmodic used in emergency departments, on nucleus pulposus annulus fibrosus cells vitro. After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered experimental groups except for control group. data obtained statistically evaluated. proliferation identified be suppressed via MTT analysis. gene expression profile...

AIM:To investigate the effects of methylphenidate (MPH), on intervertebral disc tissue (IVD) cell cultures and extracellular matrix structures.Changes in expression some important marker genes involved anabolic catabolic mechanisms IVD formation were also evaluated. MATERIAL METHODS:Primary nucleus pulposus cells (NPCs) annulus fibrosus (AFCs) isolated from tissues obtained operated patients.Cell viability proliferation tested, surface morphologies evaluated by microscopy.The expressions...

Objective: Percutaneous vertebroplasty (PVP) is widely used all over the world, especially in elderly patients for osteoporotic, traumatic, and pathological vertebral compression fractures (VCFs).Previous studies have reported incidental tumors biopsy.However, whether a routine biopsy should be performed during PVP controversial.The aim of this study was to evaluate importance treatment VCF. Materials Methods:The who underwent under sedo-analgesia single or multi-level thoracolumbar...

AIM:To introduce a traumatic brain injury (TBI) patient who underwent stem cell transplantation (SCT) in order to minimize the remaining deficiencies. MATERIAL and METHODS:This study included 29 years old male had TBI resulting from vehicle accident which took place one half ago.The participant received six doses of intrathecal, intramuscular, intravenous Wharton's jellv-derived mesenchymal cells (WJ-MSCs) at goal dose 1xl0 6 / kg respectively for each route administration months. RESULTS:No...

BACKGROUND Traumatic brain injury (TBI) is characterized by a disruption in the normal function of due to an following trauma, which can potentially cause severe physical, cognitive, and emotional impairment. Stem cell transplantation has evolved as novel treatment modality management TBI, it potential arrest degeneration promote regeneration new cells brain. Wharton’s Jelly-derived mesenchymal stem (WJ-MSCs) have recently shown beneficial effects functional recovery neurological deficits....

Spinal intradural arachnoid cysts (SIACs) are cerebrospinal fluid (CSF) sacs formed by membranes. They may be idiopathic or acquired. Treatment is resection, fenestration, cyst drainage. A 41-year-old female patient presented with myelopathy symptoms and complaints. Magnetic resonance imaging (MRI) revealed a T6-T10 dorsal cyst. laminectomy was performed an excised under surgical microscope. The contained clear liquid that surrounded transparent membrane. At 7 weeks postoperatively, the...

BACKGROUNDHypoxic-ischemic encephalopathy (HIE) is a leading cause of morbidity and mortality in the adult as well neonate, with limited options for treatment significant dysfunctionality. AIMTo investigate safety preliminary efficacy allogeneic mesenchymal stem cells (MSCs) HIE patients.19

To investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from intact intervertebral disc tissue.Cell were prepared tissues obtained six cases who had undergone surgery due to spinal trauma. Dabigatran, an active pharmacological agent, was applied annulus fibrosus (AF)/nucleus pulposus (NP) study group. After performing viability, toxicity, and proliferation tests all in control groups, surface morphologies samples evaluated. Subsequently,...

Aim: Lumbar microdiscectomy (LMD) is considered as a gold standard surgical technique for the treatment of patients with lumbar disc hernia (LDH) although various types are being developed. This study aimed to investigate safety and efficacy LMD in our clinic.Material Methods: The exclusively included 271 undergoing due LDH. Preoperative postoperative (10th day, 1st month, long-term) Visual Analog Scale (VAS) Oswestry Disability Index (ODI) scores were compared. Clinical outcomes analyzed...