A5030553008- Liver Disease Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- HIV-related health complications and treatments
- HIV, Drug Use, Sexual Risk
- Cervical Cancer and HPV Research
- Liver physiology and pathology
- Pesticide and Herbicide Environmental Studies
- Alcohol Consumption and Health Effects
- Agriculture and Farm Safety
- HIV Research and Treatment
- Polyomavirus and related diseases
- Birth, Development, and Health
- Adolescent Sexual and Reproductive Health
- Inflammasome and immune disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- HIV/AIDS drug development and treatment
- Water Quality and Resources Studies
- Respiratory Support and Mechanisms
- Diabetes and associated disorders
- Traumatic Brain Injury Research
- Pregnancy and preeclampsia studies
- Autophagy in Disease and Therapy
Emory University
2024
Atlanta VA Health Care System
2024
University of Nebraska Medical Center
2017-2023
VA Nebraska Western Iowa Health Care System
2019-2023
Nebraska Medical Center
2019-2022
This review covers some important new aspects of the alcohol-induced communications between liver parenchymal and non-parenchymal cells leading to injury development. The information exchange various cell types may promote end-stage disease progression involves multiple mechanisms, such as direct cell-to-cell interactions, extracellular vesicles (EVs) or chemokines, cytokines, growth factors contained in fluids/cell culture supernatants. Here, we highlighted role EVs derived from...
In an era of improved survival due to modern antiretroviral therapy, liver disease has become a major cause morbidity and mortality, resulting in death 15–17% human immunodeficiency virus (HIV)-infected patients. Alcohol enhances HIV-mediated damage promotes the progression advanced fibrosis cirrhosis. However, mechanisms behind these events are uncertain. Here, we hypothesize that ethanol metabolism potentiates accumulation HIV hepatocytes, causing oxidative stress intensive apoptotic cell...
The study examined rates of possible brain injury among survivors intimate partner violence. Of the 171 women screened, 91% indicated they had been hit in head or strangled, and 31% reported it happened more than six times their life. Only 35% who were strangled received medical treatment, 64% losing consciousness experienced a period being dazed confused. Organizations serving violence should routinely screen for so can obtain timely referrals neurorehabilitation services to improve quality
Although the causes of hepatotoxicity among alcohol-abusing HIV patients are multifactorial, alcohol remains least explored “second hit” for HIV-related hepatotoxicity. Here, we investigated whether metabolically derived acetaldehyde impairs lysosomes to enhance HIV-induced We exposed Cytochrome P450 2E1 (CYP2E1)-expressing Huh 7.5 (also known as RLW) cells an acetaldehyde-generating system (AGS) 24 h. then infected (or not) with HIV-1ADA them again AGS another 48 Lysosome damage was...
Liver disease is one of the leading comorbidities in HIV infection. The risk liver fibrosis development potentiated by alcohol abuse. In our previous studies, we reported that hepatocytes exposed to and acetaldehyde undergo significant apoptosis, engulfment apoptotic bodies (ABs) hepatic stellate cells (HSC) potentiates their pro-fibrotic activation. However, addition hepatocytes, under same conditions, ABs can be generated from liver-infiltrating immune cells. goal this study explore...
Alcohol abuse is common in people living with HIV-1 and dramaticallyenhances the severity of HIV-induced liver damage by inducing oxidative stress lysosomaldysfunction cells. We hypothesize that increased release extracellular vesicles(EVs) hepatocytes humanized mouse model linked to lysosome dysfunction. The study was performed on primary human hepatoma RLWXP-GFP (Huh7.5 cells stably transfected CYP2E1 XPack-GFP) validated ethanol-fed liverhumanizedfumarylacetoacetate hydrolase (Fah)-/-,...
Recently, we found that both HIV and acetaldehyde, an alcohol metabolite, induce hepatocyte apoptosis, resulting in the release of large extracellular vesicles called apoptotic bodies (ABs). The engulfment these ABs by hepatic stellate cells (HSC) leads to their profibrotic activation. This study aims establish mechanisms HSC activation after from acetaldehyde HIV-exposed hepatocytes (ABAGS+HIV). In vitro experiments were performed on Huh7.5-CYP (RLW) generate LX2 used as HSC. To ABs, RLW...
Childhood diarrhoea is a global public health problem especially in unplanned settlements of low- and middle-income countries. Different studies have associated household settlement childhood diarrhoea, but the prevalence risk factors with at community level are not clearly known.A community-based cross-sectional study was performed to determine among 480 children between 6 59 months age Mwanza city from June August 2016. Risk were determined using univariate, bivariate multivariate...
The Bureau of Labor Statistics (BLS) publishes annual statistics on occupational injuries and fatalities in the United States. BLS fatality data include all agricultural workers while non-fatal injury only cover hired employees large farms. In 2012, Central States Center for Agricultural Safety Health (CS-CASH) began collecting regional media monitoring incidents to augment national statistics. aims this report were: a) compare CS-CASH collected via print online sources reported previous...
Estrogen disrupting pesticides (EDP) are that modify estrogen activities in estrogen-producing vertebrates. A substantial amount of these has been detected human tissues, and they function directly to disrupt synthesis or effector cells. This study examines EDP’s ecological distribution across Nebraska counties its association with estrogen-related cancers (ERC). To determine the selected EDP, county-level choropleth maps were created. Moreover, EDP was tested separate linear models...
Background: Cervical cancer is among the most prevalent women worldwide and living with HIV are at increased risk, especially in a resource-limited environment. Objective: This study aimed to determine levels of awareness, knowledge, uptake, willingness screen for cervical receiving care an clinic Dodoma Regional Referral Hospital (DRRH), Tanzania. Methods: Data were collected period three weeks from July 21 August 11, 2017 using mobile phone data collection App. A total 421 Women aged 18-50...
Recent studies observed a correlation between estrogen-related cancers and groundwater atrazine in eastern Nebraska counties. However, the mechanisms of human exposure to are unclear because low concentration was counties with high cancer incidence despite having highest usage. We studied fate usage Data were collected from Quality Assessed Agrichemical Contaminant Groundwater, Parameter-Elevation Regressions on Independent Slopes Model (PRISM), water use databases. Descriptive statistics...
Approximately 3.5% of the global population is chronically infected with Hepatitis B Virus (HBV), which puts them at high risk end-stage liver disease, persistent infection potentiated by alcohol consumption. However, mechanisms underlying effects on HBV persistence remain unclear. Here, we aimed to establish in vivo/ex vivo evidence that suppresses peptides-major histocompatibility complex (MHC) class I antigen display primary human hepatocytes (PHH), diminishes recognition and clearance...
The morbidity and mortality of human immunodeficiency virus (HIV)-infection is often associated with liver disease, which progresses slowly into severe dysfunction. There are multiple insults exacerbate HIV-related injury, including HIV-associated dysregulation lipid metabolism fat turnover, co-infections hepatotropic viruses alcohol abuse. As we reported before, exposure hepatocytes to HIV metabolites causes high oxidative stress, impairs proteasomal lysosomal functions leading accumulation...
Abstract Aim This matched case–control study aimed to provide epidemiologic evidence of increased burden respiratory symptoms and pulmonary function decline among people living with human immunodeficiency virus (HIV) a history heavy alcohol consumption. Methods Cases were participants HIV (PWH; n = 75, 33%), controls without (PWoH; 150, 67%). PWH PWoH by age sex in the ratio 1:2. Eligible responded health National Health Nutrition Examination Survey questionnaire [prolonged coughs (≥3...
Background: The intention of antiretroviral therapy (ART) and regular clinic visits are to engender safe sex attitudes among HIV-infected individuals. However, this may not be the case due perceived therapeutic benefits ART result in exposure drug-resistant HIV strains. Objective: We aimed determine prevalence predict factors associated with risky sexual behaviors users a resource-limited environment. Methods: Two hundred ninety-one sexually active ART-users aged 18-50 years seeking care at...
Abstract Background Patients with HIV (PWH) sustained virologic suppression (VS) on antiretroviral therapy (ART) achieve better health outcomes and pose effectively no risk of transmitting to their sexual contacts. Adherence ART is the main predictor VS in PWH, yet adherence benchmark has been identified. The clinical utility pharmacy refill history collection unknown. We hypothesize that histories represented as a percentage days covered (PDC) will correlate PWH. Methods conducted...
Abstract Liver injury is a second cause of non-AIDS mortality in HIV-infection, and the progression to end-stage liver disease more rapid alcohol abusers. Hepatocytes comprise 80% cells are strong ethanol metabolizers. To elucidate whether hepatocytes HIV-infected, we measured kinetics HIVgag RNA (RT-PCR) p24 (Western blot) expression Huh7.5-CYP (hepatocyte-like cells) exposed HIV-1ADA presence or absence acetaldehyde-generation system (AGS). The measurements were done at days 1, 3 5...
Abstract Background Despite COVID-19 vaccine benefits, some people with HIV (PWH) might be unvaccinated. Also, whether PWH may have increased risk of severe outcomes due to immunosuppression remain inconclusive. Thus, understanding the perceived (PR) and perceptions is critical for reduced complication uptake. We applied a mixed methods approach understand PR perception vaccines among PWH. Methods recruited participants from University Nebraska Medical Center clinic. Eligible criteria...