A5075430268- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Plant and fungal interactions
- Advanced Electron Microscopy Techniques and Applications
- Vibrio bacteria research studies
- Marine Biology and Environmental Chemistry
- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Protease and Inhibitor Mechanisms
- Fungal and yeast genetics research
- Diatoms and Algae Research
- Ubiquitin and proteasome pathways
- Fermentation and Sensory Analysis
- Connective tissue disorders research
Brandeis University
2025
The Ohio State University
2020-2024
Highlights•F-BAR protein Syp1 binds F-actin and links septins using its IDR domain•F-BAR proteins Hof1 show inverse patterns of recruitment to the bud neck•Syp1 enhances Bnr1-mediated actin assembly overcomes formin inhibition by Hof1•Syp1 competitively bind differentially regulate septin architectureSummaryIn vivo functions cytoskeletons are closely intertwined, yet mechanisms underlying septin-actin crosstalk have remained poorly understood. Here, we that yeast-bud-neck-associated Fes/CIP4...
Abstract Mutations in actin-bundling protein plastin 3 (PLS3) emerged as a cause of congenital osteoporosis, but neither the role PLS3 bone development nor mechanisms underlying PLS3-dependent osteoporosis are understood. Of over 20 identified osteoporosis-linked mutations, we investigated all five that expected to produce full-length protein. One mutations distorted an actin-binding loop second domain and abolished F-actin bundling revealed by cryo-EM reconstruction interaction assays....
Intracellular pH (pHi) is a fundamental component of cell homeostasis. Controlled elevations in pHi precede and accompany polarization, cytokinesis, directional migration. dysregulation contributes to cancer, neurodegenerative diseases, diabetes, other metabolic disorders. While cytoskeletal rearrangements are crucial for these processes, only few proteins, namely Cdc42, cofilin, talin, cortactin, α-actinin, AIP1 have been documented as sensors. Here, we report that actin-bundling proteins...
Abstract Entry of Salmonella into host enterocytes strictly relies on its pathogenicity island 1 effector SipA. We found that SipA binds to F-actin in a unique mode 1:2 stoichiometry with picomolar affinity. A cryo-EM reconstruction revealed SipA’s globular core at the grove between actin strands, whereas extended C-terminal arm penetrates deeply inter-strand space, stabilizing from within. The unusually strong binding is achieved via combination fast association and very slow dissociation...