Mulberry 1-deoxynojirimycin (DNJ), a potent glucosidase inhibitor, has been hypothesized to be beneficial for the suppression of abnormally high blood glucose levels and thereby prevention diabetes mellitus. However, DNJ contents in commercial mulberry products were as low about 0.1% (100 mg/100 g dry product), implying that bioavailability might not expected. We carried out studies two directions: (1) production food-grade powder containing maximally content; (2) determination optimal dose...

The development of Alzheimer's disease (AD) biomarkers remains an unmet challenge, and new approaches that can improve current AD biomarker strategies are needed. Recent reports suggested microRNA (miRNA) profiling biological fluids has emerg

We determined red fruit anthocyanins, cyanidin-3-glucoside (Cy-g) and cyanidin-3,5-diglucoside (Cy-dg), incorporated into plasma liver of rats human by UV-HPLC. Fifteen minutes after an oral supplementation a mixture 320 mg Cy-g 40 Cy-dg/kg body weight, showed increase to maximum 1563 microg (3490 nmol) Cy-g/L 195 (320 Cy-dg/L in 0.067 (0.15 Cy-g/g trace Cy-dg together with methylated metabolites such as peonidin-3-glucoside liver. In plasma, 30 min intake (2.7 0.25 weight), average 11 (24...

Tea catechins, (–)-epigallocatechin-3-gallate (EGCg) and (–)-epigallocatechin (EGC), have been reported to suppress oxidation of plasma low density lipoprotein (LDL) in vitro. If dietary catechins can be efficiently incorporated into human blood plasma, anti-atherosclerotic effects preventing oxidative modification LDL would expected. In this study, a newly developed chemiluminescence detection-high pressure liquid chromatography (CL-HPLC) method for measuring was used the incorporation EGCg...

To investigate the absorption and metabolism of an anti-carcinogenic tea catechin, (-)-epigallocatechin-3-gallate (EGCg), in rats, a newly developed chemiluminescence-detection high-performance liq-uid chromatography (CL-HPLC) method was employed EGCg concentrations blood plasma, liver, brain, small intestinal mucosa colon were determined before after administration. The recovery EGCg, extracted consecutively with ethyl acetate methanol, 86.1 % from plasma 64.5-74.2% tissue samples. samples...

Abstract Glycolipids from edible plant sources were accurately quantified by silica‐based, normal‐phase high‐performance liquid chromatography using an evaporative light‐scattering detector. Five major glycolipid classes (acylated steryl glucoside, ceramide monohexoside, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol) separated determined with a binary gradient system consisting of chloroform methanol/water (95∶5, vol/vol) without any interference other lipid pigments. The...

The effect of green tea catechin supplementation on antioxidant capacity human plasma was investigated. Eighteen healthy male volunteers who orally ingested extract (254 mg total catechins/subject) showed 267 pmol epigallocatechin-3-gallate (EGCg) per milliliter at 60 min after administration. phosphatidylcholine hydroperoxide (PCOOH) levels attenuated from 73.7 pmol/mL in the control to 44.6 catechin-treated subjects, being correlated inversely with increase EGCg level. results suggested...

Mitochondria have long been known to play a critical role in maintaining bioenergetic status under physiological conditions. Our previous studies shown that acute heat stress increases production of mitochondrial reactive oxygen species (ROS) the skeletal muscle chickens. This increased ROS may lead nonspecific modification lipids and proteins, which then result dysfunctions. If heat-stressed chickens causes oxidative damage, changes peroxidized oxidatively modified proteins can be detected....

Abstract The extent of oxidation ethyl esters eicosapentaenoic (EPA) and docosahexaenoic acids (DHA) was compared quantitatively with that linoleate (Lo) linolenate (Ln) by oxygen uptake formation conjugated diene, hydroperoxide secondary products. EPA DHA were oxidized rapidly even at 5 C in the dark after an induction period 3–4 days, while periods Ln Lo 20 days more than 60 respectively. Oxygen 5.2 8.5 times faster Ln, Hydroperoxides are much less stable those Lo. peroxide value is not...

Phospholipid hydroperoxides (PLOOH) accumulate abnormally in the erythrocytes of dementia patients, and dietary xanthophylls (polar carotenoids such as astaxanthin) are hypothesised to prevent accumulation. In present study, we conducted a randomised, double-blind, placebo-controlled human trial assess efficacy 12-week astaxanthin supplementation (6 or 12 mg/d) on both PLOOH levels thirty middle-aged senior subjects. After weeks treatment, erythrocyte concentrations were higher 6 mg groups...

Luteolin is a naturally occurring flavone that reportedly has anti-inflammatory effects. Because most luteolin conjugated following intestinal absorption, free likely present at low levels in the body. Therefore, metabolites are presumably responsible for bioactivity. Here we confirmed glucuronides, especially luteolin-3'-O-glucuronide, major found plasma after oral administration of (aglycone) or glucoside (luteolin-7-O-glucoside) to rats. Luteolin-4'-O-glucuronide and...

Abstract A chemiluminescence - high performance liquid chromatography (CL-HPLC) system was developed for the hydroperoxide-specific detection of phosphatidylcholine hydroperoxide. In this system, detector equipped with normal phase HPLC. Luminol-cytochrome c solution used as luminescent reagent. limit 7 nmol hydroperoxide-O2 hydroperoxide could be achieved. Key Words: HPLCchemiluminescencehydroperoxidephosphatidylcholine

The quantification of phospholipid hydroperoxides in biological tissues is important order to know the degree peroxidative damage membrane lipids. For this purpose, optimal conditions for chemiluminescent simultaneous assay phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine (PEOOH) rat liver brain were determined. A chemiluminescence detection-high performance liquid chromatography (CL-HPLC) method that incorporates cytochrome c luminol as a post-column...