A5064341779- Calcium signaling and nucleotide metabolism
- Mitochondrial Function and Pathology
- Ion Channels and Receptors
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Neuroscience and Neural Engineering
- Metabolism, Diabetes, and Cancer
- Cellular transport and secretion
- Connexins and lens biology
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
Thomas Jefferson University
2025
Center for Research and Advanced Studies of the National Polytechnic Institute
2014-2022
Universidad de las Américas
2022
Cells utilize protein disaggregases to avoid abnormal aggregation that causes many diseases. Among these, caseinolytic peptidase B homolog (CLPB) is localized in the mitochondrial intermembrane space and linked human disease. Upon CLPB loss, MICU1 MICU2, regulators of calcium uniporter complex (mtCU), OPA1, a main mediator fusion, become insoluble but functional outcome remains unclear. In this work we demonstrate required maintain signalling fusion dynamics. loss results altered mtCU...
The overexpression of the Orai1 channel inhibits SOCE when using Ca2+ readdition protocol. However, we found that HeLa cells overexpressing displayed enhanced entry and a limited ER depletion in response to combination ATP thapsigargin (TG) presence external Ca2+. As these effects require an agonist TG, decided study whether phosphorylation S27/S30 residues had any role two different mutants: Orai1-S27/30A (O1-AA, phosphorylation-resistant) Orai1-S27/30D (O1-DD, phosphomimetic). Both O1-wt...