A5088703650- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- DNA Repair Mechanisms
- ATP Synthase and ATPases Research
- Hemoglobin structure and function
- Cancer, Hypoxia, and Metabolism
- Phytochemicals and Antioxidant Activities
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Ubiquitin and proteasome pathways
- Nitric Oxide and Endothelin Effects
- Polysaccharides and Plant Cell Walls
- Electron Spin Resonance Studies
- HIV-related health complications and treatments
- Aluminum toxicity and tolerance in plants and animals
- Autophagy in Disease and Therapy
- Photoreceptor and optogenetics research
- Pomegranate: compositions and health benefits
- Adipose Tissue and Metabolism
- Bioactive Compounds and Antitumor Agents
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Fungal Infections and Studies
- Tannin, Tannase and Anticancer Activities
- melanin and skin pigmentation
- Ginkgo biloba and Cashew Applications
Universidade de São Paulo
2014-2024
National Heart Lung and Blood Institute
2021-2023
National Institutes of Health
2021-2023
Cancer Genetics (United States)
2021
Universidade Estadual de Londrina
2007-2017
Jeonbuk National University
1995
The cell wall of Saccharomyces cerevisiae is an important source β-d-glucan, a glucose homopolymer with immunostimulant properties. standard methodologies described for its extraction involve acid and alkaline washings, which degrade part chains reduce the final yield. In present study, optimized methodology β-d-glucan from S. cells, involving sonication enzyme treatment, yield 11.08 ± 0.19%, was developed. high-purity (1 → 3)(1 6)-β-d-glucan derivatized to carboxymethyl-glucan (CM-G). vitro...
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by various disabling symptoms including exercise intolerance and diagnosed in the absence of a specific cause, making its clinical management challenging. A better understanding molecular mechanism underlying this apparent bioenergetic deficiency state may reveal insights for developing targeted treatment strategies. We report that overexpression
Microenvironment and transcriptional plasticity generate subpopulations within the tumor, use of BRAF inhibitors (BRAFis) contributes to rise selection resistant clones. We stochastically isolated (C1, C2, C3) from naïve melanoma found that clones demonstrated distinct morphology, phenotypic, functional profiles: C1 was less proliferative, more migratory invasive, sensitive BRAFis, dependent on OXPHOS, oxidative stress, pigmented; C2 C3 pigmented. Hydrogen peroxide plays a central role in...
IL-2 therapy is a potent inductive stimulus for nitric oxide (NO.) synthesis in mice and humans. It not yet clear whether NO. can contribute to IL-2-induced therapeutic responses. The murine skin cancer Meth A relatively resistant lymphokine-activated killer (LAK) cell killing, allowing evaluation of the role vivo, without contribution by LAK cells. Subcutaneous treatment bearing i.p. tumor increased nitrite production cells derived from ascites (63 +/- 14 microM vs 3.2 1.5 untreated...
The endosymbiotic theory posits that ancient eukaryotic cells engulfed O2-consuming prokaryotes, which protected them against O2 toxicity. Previous studies have shown lacking cytochrome c oxidase (COX), required for respiration, increased DNA damage and reduced proliferation, could be improved by reducing exposure. With recently developed fluorescence lifetime microscopy–based probes demonstrating the mitochondrion has lower [O2] than cytosol, we hypothesized perinuclear distribution of...
Abstract Genomic instability drives tumorigenesis and DNA repair defects are associated with elevated cancer. Metabolic alterations also observed during tumorigenesis, although a causal relationship between these has not been clearly established. Xeroderma pigmentosum (XP) is disease characterized by early Cells reduced expression of the XPC protein display metabolic shift from OXPHOS to glycolysis, which was linked accumulation nuclear damage oxidants generation via NOX-1. Using XP-C cells,...
Little is known about Nima‐related kinase (NEKs), a widely conserved family of kinases that have key roles in cell‐cycle progression. Nevertheless, it now clear multiple NEK members act networks, not only to regulate specific events mitosis, but also metabolic independently the cell cycle. NEK5 was shown centrosome disjunction, caspase‐3 regulation, myogenesis, and mitochondrial respiration. Here, we demonstrate interacts with LonP1, an AAA+ protease implicated protein quality control mtDNA...
Carboxymethyl-glucan (CM-G) is a soluble derivative from Saccharomyces cerevisiae (1 → 3)(1 6)-β-D-glucan. The protective efficiency of CM-G against DNA damage in cells patients with advanced prostate cancer (PCa), and undergoing Androgen Deprivation Therapy (ADT), was evaluated. scores were obtained by the comet assay, both before after treatment CM-G. reduction damage, ranging 18% to 87%, an average 59%, not related increased number leukocytes peripheral blood. results demonstrate for...
Isatin (1H-indole-2,3-dione) is a chemical found in various medicinal plant species and responsible for broad spectrum of pharmacological biological properties that may be beneficial to human health, as an anticonvulsant, antibacterial, antifungal, antiviral, anticancer agent. The aim the present study was determine vitro cytotoxic, mutagenic, apoptotic effects isatin on CHO-K1 HeLa cells using MTT viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide), micronucleus...
Summary Maintenance of the mitochondrial inner membrane potential (ΔΨM) is critical for many aspects function, including protein import and ion homeostasis. While ΔΨM loss its consequences are well studied, little known about effects increased ΔΨM. In this study, we used cells deleted ATPIF1 , a natural inhibitor hydrolytic activity ATP synthase, as genetic model hyperpolarization. Our data show that chronic increase leads to nuclear DNA hypermethylation, regulating transcription...
Botryosphaeran (BOT) is an exocellular β-d-glucan (carbohydrate biopolymer) of the (1→3;1→6)-linked type produced by Botryosphaeria rhodina MAMB-05. The cytotoxic, mutagenic, genotoxic, and protective effects this substance were evaluated in Chinese hamster lung fibroblasts (V79) rat hepatocarcinoma cells (HTC) micronucleus test (MN) comet assay. BOT was not genotoxic either cell line; it decreased clastogenic doxorubicin, H2O2, benzo[a]pyrene. These results indicate that may have potential...
The biological relevance of nitric oxide (NO) and reactive oxygen species (ROS) in signaling, metabolic regulation, disease treatment has become abundantly clear. dramatic change NO/ROS processing that accompanies a changing landscape calls for new imaging tools can provide cellular details about both [O2 ] the production species. Myoglobin oxidation to met state by is known sensor with absorbance changes visible range. We previously employed Förster resonance energy transfer read out...
Exercise training can stimulate the formation of fatty-acid-oxidizing slow-twitch skeletal muscle fibers, which are inversely correlated with obesity, but molecular mechanism underlying this transformation requires further elucidation. Here, we report that downregulation mitochondrial disulfide relay carrier CHCHD4 by exercise decreases import TP53-regulated inhibitor apoptosis 1 (TRIAP1) into mitochondria, reduce cardiolipin levels and promote VDAC oligomerization in muscle....
The XPC protein, which is mutated in xeroderma pigmentosum (XP) complementation group C (XP-C), a lesion recognition factor NER, but it has also been shown to interact with and stimulate DNA glycosylases, act as transcriptional co-activator on energy metabolism adaptation. We have previously demonstrated that XP-C cells show increased mitochondrial H2O2 production shift between respiratory complexes I II, leading sensitivity stress. Here we report marked decrease expression of the PGC-1α,...
Abstract XPC deficiency is associated with mitochondrial dysfunction, increased H2O2 production and sensitivity to the Complex III inhibitor antimycin A (AA), through a yet unclear mechanism. We found an imbalanced expression of several proteins that participate in important function phosphorylation tumor suppressor p53 Xeroderma pigmentosum complementation group C (XP-C) (XPC-null) cells compared isogenic line corrected locus wild-type (XPC-wt). Interestingly, inhibition nuclear import...