A5114608789- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Weed Control and Herbicide Applications
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Histiocytic Disorders and Treatments
- Genomics and Chromatin Dynamics
- Allelopathy and phytotoxic interactions
- Indoor Air Quality and Microbial Exposure
- Seed Germination and Physiology
- Molecular Biology Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- Cutaneous lymphoproliferative disorders research
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Plant tissue culture and regeneration
- Genomic variations and chromosomal abnormalities
- Acute Kidney Injury Research
- Glioma Diagnosis and Treatment
- Plant Molecular Biology Research
- Renal and related cancers
- Animal Genetics and Reproduction
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
National Institute of Environmental Health Sciences
2020-2024
Research Triangle Park Foundation
2024
North Carolina State University
2011-2020
North Central State College
2020
University of Arkansas for Medical Sciences
2018-2019
Abstract The Long-read RNA-Seq Genome Annotation Assessment Project Consortium was formed to evaluate the effectiveness of long-read approaches for transcriptome analysis. Using different protocols and sequencing platforms, consortium generated over 427 million sequences from complementary DNA direct RNA datasets, encompassing human, mouse manatee species. Developers utilized these data address challenges in transcript isoform detection, quantification de novo detection. study revealed that...
Eukaryotes use a temporally regulated process, known as the replication timing program, to ensure that their genomes are fully and accurately duplicated during S phase. Replication programs predictive of genomic features activity considered be functional readouts chromatin organization. Although have been described for yeast animal systems, much less is about temporal regulation plant DNA or its relationship genome sequence structure. We used thymidine analog, 5-ethynyl-2′-deoxyuridine, in...
Abstract Formaldehyde (FA) is a simple biological aldehyde that produced inside cells by several processes such as demethylation of DNA and proteins, amino acid metabolism, lipid peroxidation one carbon metabolism (1-C). Although accumulation excess FA in known to be cytotoxic, it unknown if an increase level might associated with mitochondrial dysfunction. We choose use primary human fibroblasts culture (foreskin, FSK) physiological model gain insight into whether the affect cellular...
Abstract Pseudomonas aeruginosa is a major cause of nosocomial infections and the leading chronic lung in cystic fibrosis obstructive pulmonary disease patients. Antibiotic treatment remains challenging because P. resistant to high concentrations antibiotics has remarkable ability acquire mutations conferring resistance multiple groups antimicrobial agents. Here we report that when plated on ciprofloxacin (cipro) plates, majority cipro-resistant (ciproR) colonies observed at after 48 h...
With 4 tables Abstract The 2,4‐dihydroxy‐1,4‐benzoxazin‐3‐one (DIBOA) content of Secale cereale is strongly associated with allelopathy. This has led to interest in developing allelopathic cultivars increased DIBOA improve weed control this important cover crop. Objectives study were determine heritability estimates for rye and the utility gas chromatography (GC) as a screening tool allelopathy breeding programme. A synthetic population half‐sib families varying production was analysed....
The selection and firing of DNA replication origins play key roles in ensuring that eukaryotes accurately replicate their genomes. This process is not well documented plants due large measure to difficulties working with plant systems. We developed a new functional assay label map very early replicating loci must, by definition, include at least subset origins. Arabidopsis (Arabidopsis thaliana) cells were briefly labeled 5-ethynyl-2′-deoxy-uridine, nuclei subjected two-parameter flow...
Early detection of neurological conditions is critical for timely diagnosis and treatment. Identifying cellular-level changes essential implementing therapeutic interventions prior to symptomatic disease onset. However, monitoring brain tissue directly through biopsies invasive high-risk. Bodily fluids such as blood or cerebrospinal fluid contain information in many forms, including proteins nucleic acids. Particularly, cell-free DNA (cfDNA) has potential a versatile biomarker. Yet, our...
Early detection of neurological conditions is critical for timely diagnosis and treatment. Identifying cellular-level changes essential implementing therapeutic interventions prior to symptomatic disease onset. However, monitoring brain tissue directly through biopsies invasive poses a high risk. Bodily fluids such as blood or cerebrospinal fluid contain information in many forms, including proteins nucleic acids. In particular, cell-free DNA (cfDNA) has potential versatile biomarker. Yet,...
Renal proximal tubule epithelial cells (RPTECs) are a primary site for kidney injury. We created two RPTEC lines from CD-1 mice immortalized with hTERT (human telomerase reverse transcriptase) or SV40 LgT antigen (Simian Virus 40 Large T antigen). Our hypothesis was that low-level, repeated exposure to subcytotoxic levels of 0.25-2.5 μM cisplatin (CisPt) 12.5-100 aflatoxin B1 (AFB1) would activate distinctive genes and pathways in these differently cell lines. RNA-seq showed only responded...
The cancer hazard associated with lifetime exposure to radiofrequency radiation (RFR) was examined in Sprague Dawley (SD) rats at the Ramazzini Institute (RI), Italy. There were increased incidences of gliomas and cardiac schwannomas. translational relevance these rare rat tumors for human disease is poorly understood. We genetic alterations RFR-derived through molecular characterization important genes relevant gliomagenesis. A targeted next-generation sequencing (NGS) panel designed based...
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