The cell wall of Saccharomyces cerevisiae is an important source β-d-glucan, a glucose homopolymer with immunostimulant properties. standard methodologies described for its extraction involve acid and alkaline washings, which degrade part chains reduce the final yield. In present study, optimized methodology β-d-glucan from S. cells, involving sonication enzyme treatment, yield 11.08 ± 0.19%, was developed. high-purity (1 → 3)(1 6)-β-d-glucan derivatized to carboxymethyl-glucan (CM-G). vitro...

Considering the limited availability of technology for production rice vinegar and also due to potential consumer product market, this study aimed use alcoholic fermented (rice wine (Oryza sativa L.)) production. An solution with 6.28% (w/v) ethanol was oxidized by a submerged fermentation process produce vinegar. The acetic acid occurred at 30 ± 0.3°C in FRINGS® Acetator (Germany) followed through 10 cycles. had total acidity 6.85% (w/v), 0.17% alcohol 1.26% minerals 1.78% dry extract....

Probiotics are living microorganisms, which when administered in adequate amounts confer health benefits on the host through a beneficial influence intestinal microbiota related to competition and antagonistic immunological effects. Thus, objective of this study was development effervescent products (tablets powder) supplemented with probiotics Lactobacillus acidophilus Saccharomyces boulardii, identification best formulation terms viability these microorganisms. The physical properties...

The oyster mushroom, Pleurotus ostreatus, cultivated in solid state on sugarcane bagasse–wheat bran (5:1) medium the presence of veratryl alcohol resulted an increased production fruiting body at earlier times compared to when fungus was grown absence alcohol. results indicate a new physiological role for stimulating formation. Veratryl also stimulated laccase during mycelial growth stage. Evidence is presented that laccases were involved development body.

Sugar cane silage has a potential for animal feeding, but uncontrolled growth of undesirable microorganisms may cause nutritional losses and affect the productivity health. The objective this work was to evaluate microbiological quality chemical composition ensiled sugar with without additives after 30 days fermentation. Yeasts, filamentous fungi distinct groups bacteria were enumerated by plate count methods analyzes comprised dry matter, crude protein, fiber content, lignin, pH....

The biomass of yeast after beer production is a raw-material for cell components extraction, including mannoproteins. present study evaluated the using viability spent brewer's Saccharomyces sp. obtainment extract containing mannoprotein. extraction was conducted by Box-Behnken 33 incomplete design, variables temperature (75, 85 and 95ºC), time (5, 7 9h) concentration wall in suspension (10, 15 20%). residual ethanol fermentation doesn't have interference obtaining highest rate 4.08%,...

b-glucans are polysaccharides that constitute the structure of cell wall yeast, fungi and some cereals, which differs each other by linkages between glucose units. An important source these polymers is Saccharomyces cerevisiae wall, a yeast widely used in industrial processes fermentation. The b-glucan considered to be modifier biological response due its immunomodulator potential. When it recognized specific cellular receptors, have ability enhance host's immune response. Other beneficial...

Carboxymethyl-glucan (CM-G) is a soluble derivative from Saccharomyces cerevisiae (1 → 3)(1 6)-β-D-glucan. The protective efficiency of CM-G against DNA damage in cells patients with advanced prostate cancer (PCa), and undergoing Androgen Deprivation Therapy (ADT), was evaluated. scores were obtained by the comet assay, both before after treatment CM-G. reduction damage, ranging 18% to 87%, an average 59%, not related increased number leukocytes peripheral blood. results demonstrate for...

Carboxymethyl-glucan (CM-G) is a water-soluble derivative of β(1→3)(1→6) glucan, well-known immunostimulant and antioxidant compound. In this experimental, randomized placebo-controlled study, the effects oral CM-G intake over 60-day period on peripheral blood, cholesterol, glycemic index malondialdehyde (MDA) levels healthy men was assessed. The obtained from spent brewer’s yeast (S. uvarum) with DS 0.8 molecular weight 2.2 × 105 Da. Following administration, no changes were observed in red...

Foi avaliada a atividade antimicrobiana de uma cultura probiótica comercial, o Lactobacillus acidophilus La5, frente ao crescimento dois microrganismos patogênicos veiculados por alimentos, Escherichia coli e Staphylococcus aureus.O antagonismo da in vitro foi avaliado utilizando tanto Metodologia Multicamadas quanto Difusão em Ágar.Os resultados demonstraram que substância inibidora presente no sobrenadante é extracelular difusível.O período incubação bactéria lática caldo MRS, 37ºC...

The insect control is carried out mostly by chemical products, whose cumulative effects cause serious losses to environmental and human health, highlighting rapid selection of resistant insects. Biological entomopathogenic bacteria an efficient alternative, mainly due high specificity, absence resistance in the target insects low environment residual effect. Bacillus thuringiensis a Gram-positive spore-forming bacterium that produces parasporal crystal protein toxic for many species. Despite...

This study was designed to investigate the effects of a newly synthesized carboxymethyl-glucan (CM-G) on blood pressure (BP), baroreflex sensitivity (BRS) and sympathetic vascular modulation in renovascular hypertensive rats. Male Wistar rats were divided into four groups: Sham (n = 10); 2K1C (subjected renal artery clipping induce hypertension, n Sham+CM-G (treated with CM-G, 7) 2K1C+CM-G n=7). The daily treatment CM-G (40 mg/kg) performed for 2 weeks. Blood pressure, heart rate (HR),...

Bacillus thuringiensis israelensis (Bti) is a sporulating Gram-positive bacterium that produces protein crystals with insecticide activity against Diptera. The aim of the present work was to optimise culture medium for this bacterium, based on mathematical and statistical concepts (factorial designs response surface methodology). variables studied were carbon nitrogen source concentrations. main analysed toxicity, evaluated by means bioassay Aedes aegypti. nutrient sources first selected...

Lactobacilli and bifidobacteria have a long history of safe use in foods. These bacteria biotechnological characteristics interest such as the inhibition pathogens. In this work, two lactobacilli strain bifidobacterium isolated from human gut were evaluated concerning to their ability inhibit pathogenic microorganisms foods by diffusion agar tests. Moreover, we assessed metabolites produced culture broth under static shaking growth simulate anaerobiosis aerobiosis conditions, respectively....

The objective of this study was to evaluate the chito-oligosaccharide (COS) against two growth promoter antibiotics, colistin and lincomycin, with respect performance, incidence diarrhea, visceral characteristics, morphometry, serum immunoglobulin levels (IgA, IgG, IgM). A total 96 Pen Ar Lan® piglets (48 barrows 48 females), weaned at 17 days body weight (BW) 5.33 ± 0.37 kg, were subjected evaluation performance serum. Twenty-four animals, females, 35-day-old BW 6.86 0.64 used for...

Rice wine was made from broken rice (by-product of processing), which hydrolyzed by amylolytic enzymes, producing syrup with high content dextrose equivalent (51.04% DE). The must presented 164 g/L glucose (15 ºBx) and pH 2.67 fermented for 22 h at 25C Saccharomyces cerevisiae. Fermented alcoholic (rice wine) reached yield value 74.94% (Y1), 91.71% (Y2) productivity (Pp) 2.85 g/Lh in alcohol, alcohol concentration 62.8 g/L, 28.0 glucose, 2.5 °Bx 3.0. kinetic parameters – Yp/s 27.75%, μN...

The carboxymethylglucan (CMG) is a soluble molecule, composed of glucopyranosyl linked by ?(1-3) e ?(1-6), which can activate the immune system host. purpose this study was evaluate productive and immunological characteristics 192 poultry (Gallus domesticus) COBB line received feds containing 0%, 0,025%, 0,050% 0,075% CMG from Saccharomyces cerevisiae added in corn flour. All were immunized against Newcastle disease at each treatment 3 poultries randomly chosen intramuscular 3, 7 14 days. It...

A presente pesquisa avaliou a ação mutagênica e antimutagênica de um biopolímero glucose extraído da Agrobacterium radiobacter (Biopolímero radiobacter). O experimento foi realizado com camundongos Swiss machos divididos em oito grupos. tratamento o por gavage dose única concomitante uma solução tampão fosfato nos grupos avaliação mutagenicidade, ou ao agente indutor danos no DNA, ciclofosfamida, na concentração 50 mg/kg (peso corpóreo - p.c.), antimutagenicidade. Utilizou-se teste...

Gummy candies are nutritionally poor foods; however, they have a large consumer market. Enhancing these products with nourishing and/or functional matrices is challenge. This study aimed to develop the features of gummy candy probiotic, containing Bifidobacterium lactis HN019, and evaluate its effectiveness in inhibiting Streptococcus mutans UA159. The was suitable for probiotic development, whose viable bacterial count 8.54 Log CFU/g ready-to-eat product; high viability maintained during...

Yeasts discarded in industrial processes can be used as a nutritional supplement and to extract cellular components with biotechnological aims. In this study, the humoral immune response of Swiss mice treated mannoproteins (MP) from yeast Saccharomyces uvarum was evaluated. The were MPs at different doses times inoculated 2% sheep red blood cells. An increase total Ig 100 μg MP time immunization or 24 h before observed primary response; secondary response, an values for all groups, IgG (100...

The alcoholic fermentation process with agitation/static, followed by the acetic submerged in banana was evaluated. Kinetics parameters of for maximum ethanol/glycerol metabolites agitated 29 and 27 h, static 47 45 respectively. For fermentation, kinetics were medium time 39.9 acid yield 53.1% productivity 0.216 g/Lh. Wines from agitated/static presented 5.73 6.81% (v/v) alcohol content, Wine obtained higher concentrations volatile compounds. vinegar showed 49.2 g/L esters than wine. wine...

This study aimed to evaluate the performance of laying hens fed different levels enzyme complex (phytase, cellulase, pectinase, protease, amylase, beta-glucanase e xylanase). 328 were used Shaver White strain at 60 weeks age. The birds distributed into five treatments, involving (0, 50, 100, 150 and 200 ppm). We evaluated hens, egg shell quality, internal pH viscosity intestinal contents, dry matter mineral in excreta microbiology contents small intestine. results showed that addition diets...