The commercial enzyme (E.C. = 3.2.1.23) from Kluyveromyces lactis (liquid) and Aspergillus oryzae(lyophilized) was investigated for its hydrolysis potential in lactose substrate, UHT milk, skimmed milk at different concentrations (0.7; 1.0 1.5%), pH values (5.0; 6.0; 6.5 7.0), temperature (30; 35; 40 55 ºC). High rates were observed the K. 7.0 ºC, A. oryzae 5.0 ºC. showed significantly higher when compared to oryzae. effect of β-galactosidase concentration on than all temperatures tested....

The production of ethanol and beta‐galactosidase by Kluyveromyces marxianus Saccharomyces fragilis strains grown in cheese whey was evaluated. conditions for fermentation 50 g/L (3.3% lactose) 150 (8.8% were 100 rpm 24 h at 30, 35 40 °C. IZ 275 8.8% lactose °C resulted 3.90% ethanol. CCT 3172 showed higher beta‐galactosidase, 1.10 U/mg, 30 Therefore, the choice cultivation most suitable species is important obtaining high yields products interest.

Neste trabalho, verificou-se a viabilidade de Lactobacillus casei em flan chocolate, além sua sobrevivência condições que simulam o trato gastrointestinal humano. Após 1 e 15 dias da produção, população L. presente na sobremesa foi avaliada, submetido, seguida, uma solução ácida, durante 30, 60, 90 120 minutos, sais biliares, por um período 150 minutos. cada desses tempos, observada. Os resultados foram avaliados meio análise variância (ANOVA) teste Tukey. A no maior 109 UFC/g vida...

This study sought to create a better fermentation medium maximize lactase production by Saccharomyces fragilis IZ 275 using different carbon sources, including reconstituted powdered cheese whey. A factorial design 24 was applied evaluate the significant effects of variables which compose medium. Then, steepest descent-ascent obtain maximum activity. Rotational Central Composite Design (RCCD) made optimize We verified that whey, by-product dairy industry, can be employed as an excellent...

Lactobacilli and bifidobacteria have a long history of safe use in foods. These bacteria biotechnological characteristics interest such as the inhibition pathogens. In this work, two lactobacilli strain bifidobacterium isolated from human gut were evaluated concerning to their ability inhibit pathogenic microorganisms foods by diffusion agar tests. Moreover, we assessed metabolites produced culture broth under static shaking growth simulate anaerobiosis aerobiosis conditions, respectively....

The production of value-added products could be a valuable option for cheese wastewater management. However, this kind study cannot just focus alone on getting the final product. This also necessitates studies dynamics bioprocesses. With these as background, present investigation aimed at evaluating buffering capacity deproteinized whey and effect temperature nitrogen source ethanol yields from it. batch fermentation conditions used to evaluate were temperatures 30, 35, 40°C pH 4.5, 5.0,...

Este artigo analisa o letramento digital de alunos ingressantes no ensino superior, destacando os aspectos do conhecimento computacional, comunicacional e informacional. Realizou-se uma pesquisa descritiva exploratória quantitativa analítica e, como técnica específica para obter as informações requeridas, utilizou-se um questionário composto por 12 questões sobre relacionados à competência informacional, adaptadas dos estudos Ribeiro Behar (2013), além campos referentes ao perfil...

Aspergillus oryzae grown in cheese whey has the ability to produce beta-galactosidase. The objective of this work was define parameters for determination cell permeabilization and extraction enzyme from CCT 0977 biomass, with high enzymatic activity. Box–Behnken design used determine beta-galactosidase conditions. fermentation carried out a period 5 days at 28°C, having as substrate deproteinized whey. To effect variables on activity, activity determined by lactose hydrolysis reaction. most...

The permeabilization was used to transform microorganisms in cell biocatalysts with high enzymatic activity. Saccharomyces fragilis IZ 275 yeast cells were permeabilized ethanol, as permeabilizing agent. To optimize the conditions design of Box-Behnken 15 trials (3 central points). independent variables and their levels ethanol (29, 32 35%), temperature (15, 20 25°C) time 25 min). answer (Y) function has beta-galactosidase activity (U mg-1). optimum for obtaining a observed 35%...

Neste trabalho, verificou-se a viabilidade de Lactobacillus casei em flan chocolate, alem sua sobrevivencia condicoes que simulam o trato gastrointestinal humano. Apos 1 e 15 dias da producao, populacao L. presente na sobremesa foi avaliada, submetido, seguida, uma solucao acida, durante 30, 60, 90 120 minutos, sais biliares, por um periodo 150 minutos. cada desses tempos, observada. Os resultados foram avaliados meio analise variância (ANOVA) teste Tukey. A no maior 109 UFC/g vida...

Whey is the liquid that results from coagulation of milk during cheese manufacture. Cheese whey also an important environmental pollution source. The present experiment sought to compare β-galactosidase (EC 3.2.1.23) production by Aspergillus oryzae deproteinized and un-deproteinized CWP solutions. was produced submerged fermentation in or To determine activity enzyme, a reaction mixture containing cell-free extract ortho-Nitrophenyl-β-galactoside (ONPG) used. indicated induction greater...

The cheese whey shows an organic nutrient charge that can be used to obtain metabolites of interest by biotechnology microorganisms. Thus, fermentative processes for enzyme production, in particular beta-galactosidase becomes feasible. plays important role the biotech food industry milk and dairy products with low lactose content consumption intolerant individuals. objective this work was determine activity concentrated (CBG) permeabilized cells (PC) both obtained from Saccharomyces fragilis...

Abstract The purpose of the study was to determine (1) whether baking process (a unit operation) – applied produce leavened (LFB) and unleavened bread (UFB) modifies bioactivity whey protein (WP) added in these portions (2) how can change textural parameters formulations. Reducing power activity (antioxidant potential) food matrix evaluated using phosphomolybdenum method. Textural hardness, cohesiveness, springiness, resilience, chewiness gumminess were carried out on a texture analyzer. In...

This work aims to develop a freeze-dried milk powder with low lactose content and evaluate the percentage composition, microbiological analysis, soy lecithin temperature effect on solubility, dispersibility, wettability of final product. According results, treatments without hydrolysis showed significant differences in moisture (3.96% 2.63%), ash (8.92% 10.0%), (1.57% 61.46%), glucose (33.79% 0.81%) contents. In both treatments, no contaminant was observed. For high values most appropriate...

Milk in Brazil is produced mainly by Holstein, Girolando and crossbred cattle. Animal breed number of lactations are factor that can affect productivity features the milk. The purpose this research was to evaluate influence lactation on milk productivity, physicochemical composition, Somatic Cell Count (SCC) from These data were collected a farm, north Parana state, 2006 2011. relations among parameters evaluated correlation test effects (1-6) variance analysis Tukey’s test. Holstein showed...

Bovinos holandeses, girolandos e mesticos sao os principais responsaveis pela producao de leite no Brasil. A raca o numero lactacoes fatores que podem afetar essa produtividade, bem como caracteristicas do leite. O objetivo desse trabalho foi avaliar a influencia grupo racial sobre leiteira, composicao fisico-quimica contagem celulas somaticas (CCS) bovinos Holandeses, Girolandos mesticos. Dados uma propriedade rural, norte Parana, foram coletados entre anos 2006 2011. relacao parâmetros...

O soro de queijo é o principal subproduto da indústria laticínios e a alta demanda biológica química oxigênio (DBO DQO) pode causar vários problemas ambientais. Estudos recentes apontam os potenciais usos biotecnológicos do queijo, como meio fermentação, para produção β-galactosidase. A enzima muito importante hidrólise lactose em galactose glicose, monossacarídeos mais digeríveis pelo organismo humano. As microalgas podem produzir β-galactosidase através processos fermentativos. objetivo...

The participation of the graduate student in Undergraduate Research Program provides learning scientific method and can improve their performance particularly master's doctoral courses.The aim this study was to investigate production students who participated strict sense research programs.To answer question posed developed a documental descriptive research.We analyzed curricula Lattes platform enrolled six UNOPAR Masters Courses from July August 2015.To carry out proposed analysis were...

Microalgas apresentam grande potencial de utilização nos setores industriais devido à capacidade destes microrganismos fotossintetizantes em produzir biomassa rica compostos biologicamente ativos. Baseado neste contexto, o presente trabalho teve por objetivo realizar um levantamento patentes visando apresentar, a nível mundial, panorama das tendências tecnológicas do uso microalgas além identificar os países que mais registram sobre bioativos diversas áreas interesse. Foram analisados...

Estudo sobre a pesquisa na graduação como princípio formativo para Educação Superior. A universidade lócus do conhecimento tem uma atividade preponderante de forma indissociável com ensino e extensão. Instituição Superior expressa suas intenções em planejamento gestão institucional o desenvolvimento atividades no alcance seus fins. Objetivamos analisar papel da porta entrada considerando sua história Brasil os modelos universidade. Como procedimento metodológico optamos por abordagem...

O estudo teve como objetivo avaliar qualitativamente o efeito inibitório de extratos Chlorella sorokiniana obtidos pela extração com diferentes solventes orgânicos polaridades crescentes em bactérias patogênicas. A microalga C. cepa IPR 7104 foi liofilizada e submetida às extrações os respectivos solventes: hexano, diclorometano, acetato etila metanol para posterior ensaio inibitório. Para teste inibição, as Escherichia coli, Salmonella enterica Serratia marcescens foram cultivadas meio...