A5068864750- Melanoma and MAPK Pathways
- melanin and skin pigmentation
- Heme Oxygenase-1 and Carbon Monoxide
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gout, Hyperuricemia, Uric Acid
- Protein Degradation and Inhibitors
- Antimicrobial Peptides and Activities
- Essential Oils and Antimicrobial Activity
- Natural product bioactivities and synthesis
- TiO2 Photocatalysis and Solar Cells
- Redox biology and oxidative stress
- Cutaneous Melanoma Detection and Management
- Computational Drug Discovery Methods
- Skin Protection and Aging
- Sulfur Compounds in Biology
- Advanced Photocatalysis Techniques
- Dermatology and Skin Diseases
- Histone Deacetylase Inhibitors Research
- Sesquiterpenes and Asteraceae Studies
- Vanadium and Halogenation Chemistry
- Aldose Reductase and Taurine
- Indoor Air Quality and Microbial Exposure
- Vitamin C and Antioxidants Research
- Biochemical and Structural Characterization
- Fungal Infections and Studies
Universidade de São Paulo
2013-2024
Moffitt Cancer Center
2023-2024
Universidade Estadual Paulista (Unesp)
2021
Centro Universitário de João Pessoa
2021
Universidade Presbiteriana Mackenzie
2010-2011
Urate hydroperoxide is a product of the oxidation uric acid by inflammatory heme peroxidases. The formation urate might be key event in vascular inflammation, where there large amount and oxidizes glutathione sulfur-containing amino acids expected to react fast toward reactive thiols from peroxiredoxins (Prxs). kinetics for cytosolic 2-Cys Prx1 Prx2 revealed that these enzymes at rates comparable with hydrogen peroxide. second-order rate constants reactions were 4.9 × 105 2.3 106 m-1 s-1...
Abstract Melanomas can adopt multiple transcriptional states. Little is known about the epigenetic drivers of these cell states, limiting our ability to regulate melanoma heterogeneity. Here, we identify stress-induced HDAC8 activity as driving brain metastasis development. Exposure melanocytes and cells stresses increases activation leading a neural crest-stem state an amoeboid, invasive phenotype that seeding brain. Using ATAC-Seq ChIP-Seq show increased alters chromatin structure by...
Microenvironment and transcriptional plasticity generate subpopulations within the tumor, use of BRAF inhibitors (BRAFis) contributes to rise selection resistant clones. We stochastically isolated (C1, C2, C3) from naïve melanoma found that clones demonstrated distinct morphology, phenotypic, functional profiles: C1 was less proliferative, more migratory invasive, sensitive BRAFis, dependent on OXPHOS, oxidative stress, pigmented; C2 C3 pigmented. Hydrogen peroxide plays a central role in...
Melanoma is the most aggressive type of skin cancer. Despite available therapies, minimum residual disease still refractory. Reactive oxygen and nitrogen species (ROS RNS) play a dual role in melanoma, where redox imbalance involved from initiation to metastasis resistance. Redox proteins modulate by controlling ROS/RNS levels immune response, proliferation, invasion, relapse. Chemotherapeutics such as BRAF MEK inhibitors promote oxidative stress, but high amounts with robust antioxidant...
Uric acid is the end product of purine metabolism in humans and an alternative physiological substrate for myeloperoxidase. Oxidation uric by this enzyme generates free radical urate hydroperoxide, a strong oxidant potentially bactericide agent. In study, we investigated whether oxidation production hydroperoxide would affect killing activity HL-60 cells differentiated into neutrophil-like (dHL-60) against highly virulent strain (PA14) opportunistic pathogen Pseudomonas aeruginosa. While...
The essential oils from leaves and stem barks Drimys brasiliensis Miers (Winteraceae) were individually obtained by hydrodistillation their compounds characterized use of GC/FID GC/MS. main identified derivatives monoterpenes (leaves 4.31% 90.02%) sesquiterpenes 52.31% 6.35%). Additionally, the sesquiterpene polygodial was isolated hexane extract D. after chromatographic steps spectroscopic means, mainly NMR. Aiming evaluation anti-inflammatory potential, crude subjected to bioassays...
Urate hydroperoxide is a strong oxidant generated by the combination of urate free radical and superoxide. The formation as an intermediate in oxidation potentially responsible for pro-oxidant effects inflammatory disorders, protein degradation, food decomposition. To understand molecular mechanisms that sustain harmful oxidative stress related conditions, we report detailed structural characterization reactivity toward biomolecules. was synthesized photo-oxidation myeloperoxidase/hydrogen...
Paracoccidioidomycosis is a systemic fungal disease, considered endemic in Latin America. Its etiological agents, fungi of the Paracoccidioides complex, have restricted geographic habitat, conidia as infecting form, and thermo-dimorphic characteristics. Polymorphonuclear neutrophils (PMNs) are responsible for an important defense response against fungus, releasing Neutrophil Extracellular Traps (NETs), which can wrap destroy yeasts. However, it has been described that some pathogens able to...
Here, we verify the depigmenting action of Pouteria macrophylla fruit extract (EXT), incorporate it into a safe topical microemulsion and assess its effectiveness in 3D pigmented skin model. Melanocytes-B16F10- were used to EXT effects on cell viability, melanin synthesis, synthesis-related gene transcription factor expression, which demonstrated 32% 50% reduction intra extracellular content, respectively. The developed was composed Cremophor EL®/Span 80 4:1 (w/w), ethyl oleate, pH 4.5 HEPES...
Abstract Our previous work using a melanoma progression model composed of melanocytic cells (melanocytes, primary and metastatic samples) demonstrated various deregulated genes, including few known lncRNAs. Further analysis was conducted to discover novel lncRNAs associated with melanoma, candidates were prioritized for their potential association invasiveness or other metastasis‐related processes. In this sense, we found the intergenic lncRNA U73166 (ENSG00000230454) decided explore its...
EDTA is an important metal sequestering agent in industrial processes and household products, but also emerging pollutant a challenge for wastewater treatment due to its high resistance biodegradation. Conventional strategies recovering metals from the chelates require previous oxidation step. Herein, P25 TiO2 photocatalyst was applied assemble nanoparticulate films on gold (model conducting substrate) with highly reproducible photocatalytic activity toward Cu(II)-EDTA chelate)...
STRUCTURES, PROPERTIES AND PERSPECTIVES.The increasing incidence of microbial infections, high toxicity, and level resistance associated with conventional antibiotics has created a need for new drugs.Antimicrobial peptides (AMPs) constitute promising alternative and/or an important source knowledge given their ability to inhibit the growth kill bacteria, fungi, parasites viruses through mechanisms action different from those non-peptide drugs.This review focused on this class organic...
Aim: To enhance the tretinoin (TRE) safety profile through encapsulation in nanostructured lipid carriers (NLC). Materials & methods: NLC-TRE was developed using a 23 experimental factorial design, characterized (HPLC, dynamic light scattering, differential scanning calorimetry, x-ray diffraction analysis, transmission electron microscopy, cryo-transmission microscopy) and evaluated by vitro studies healthy volunteers. Results: The presented spherical structures, average particle size of 130...
The essential oils from leaves and stem barks of Drimys brasiliensis Miers (Winteraceae), collected in July December 2008, were analyzed by GC/FID GC/MS. bark composed mainly monoterpenes (July, 45 +/- 3%; December, 92 4%), while the showed predominance sesquiterpenes 47 1%; 55 1%). variation relative amount constituents could be associated, at least part, to several microclimatic parameters such as precipitation, temperature and/or phenological state, which different for each collection D....
Abstract Introduction: Mutations in the small GTPase NRAS are second most common oncogenic driver cutaneous melanoma, being found ~25% of cases. Targeted therapy strategies currently lacking for NRAS-mutant melanoma. Here, we characterized anti-tumor activity and immune effects RMC-7977, a RASMULTI(ON) preclinical tool compound, representative investigational agent RMC-6236, mouse human models Methods: The vitro potency RMC-7977 was determined using melanoma cell lines. Recovery signaling...
Abstract The impact of advanced glycation end products (AGEs) accumulation is associated with the development and exacerbation many degenerative processes disorders, including aging cancer. AGEs in skin leads to stiffness loss elasticity by building up connective tissue during process. This process has implications extracellular matrix, affecting adhesion, migration, invasiveness potential melanoma cells. Furthermore, tumor correlated mechanical stiffness, contributing high metastatic...
The endogenous estradiol derivative 2-Methoxyestradiol (2-ME) has shown good and wide anticancer activity but suffers from poor oral bioavailability extensive metabolic conjugation. However, its sulfamoylated derivative, 2-methoxyestradiol-3,17-O,O-bis-sulfamate (STX140), superior potential as a therapeutic agent, acts by disrupting microtubule polymerization, leading to cell cycle arrest apoptosis in cancer cells possesses much better pharmaceutical properties. This study investigated the...
Sepsis is a complex infectious syndrome in which neutrophil participation crucial for patient survival. Neutrophils quickly sense and eliminate the pathogen by using different effector mechanisms controlled metabolic processes. The mammalian target of rapamycin (mTOR) pathway an important route regulation, its role metabolism has not been fully understood yet, especially importance mTOR 2 (mTORC2) functions. In this study, we observed that loss Rictor (mTORC2 scaffold protein) primary...