A5068613931- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Venomous Animal Envenomation and Studies
- Healthcare and Venom Research
- Bacteriophages and microbial interactions
- Protein Hydrolysis and Bioactive Peptides
- Entomological Studies and Ecology
- Pain Mechanisms and Treatments
- Allergic Rhinitis and Sensitization
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Hemoglobin structure and function
- Botulinum Toxin and Related Neurological Disorders
- Piperaceae Chemical and Biological Studies
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- Food Allergy and Anaphylaxis Research
- Radiopharmaceutical Chemistry and Applications
- Animal Virus Infections Studies
- Neuropeptides and Animal Physiology
- Machine Learning in Bioinformatics
- Click Chemistry and Applications
- Rabies epidemiology and control
- Botanical Research and Chemistry
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2023
Universidade de São Paulo
2004-2017
Instituto Butantan
2012-2016
National University of San Marcos
2000-2002
Introduction Considering the likely need for development of novel effective vaccines adapted to emerging relevant CoV-2 variants, increasing knowledge epitope recognition profile among convalescents and afterwards vaccinated with identification immunodominant regions may provide important information. Methods We used an RBD peptide microarray identify IgG IgA binding in serum 71 COVID-19 18 individuals. Results found a set antibody epitopes, each recognized by more than 30% tested cohort,...
Sublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific with peptide vaccines far less, but it of great interest in the treatment allergic reactions, as precludes drawbacks allergen extracts. The ideal candidates would bind IgG, blocking IgE-binding. To better elucidate IgE and IgG4 epitope profiles during SLIT, sequences main allergens, Der p 1, 2, 5, 7, 10, 23 Blo t 6, 12, 13, were included a 15-mer microarray tested against pooled sera...
This review begins with a brief discussion of the biological importance and chemical features peptides.A description existing synthetic methods follows emphasis on basic aspects enzymatic syntheses.Techniques used to purify characterize synthesized peptides are also discussed.Finally, few applications final products in chemistry, biochemistry, immunology medicine presented, such as identification quantification naturally occurring peptides, inspection structureactivity relationships,...
Abstract Glycine‐rich proteins (GRPs) serve a variety of biological functions. Acanthoscurrin is an antimicrobial GRP isolated from hemocytes the Brazilian spider Acanthoscurria gomesiana . Aiming to contribute knowledge secondary structure and stepwise solid‐phase synthesis GRPs' glycine‐rich domains, we attempted prepare G 101 GGLGGGRGGGYG 113 GGGGYGGGYG 123 GGY 126 GGGKYK 132 ‐NH 2 , acanthoscurrin C‐terminal amidated fragment. Although theoretical prediction did not indicate high...
Abstract The preparation of small‐sized protected peptide acids related to cholecystokinin and gomesin was attempted using peptide‐Kaiser oxime resins (KOR) as starting materials. For comparison, peptide‐2‐Cl‐trityl resin (CLTR) also employed. While detachment from KOR achieved through the ester bond hydrolysis mediated by DBU, hydroxide ion or Ca +2 ion, release CLTR accomplished acid‐catalysed peptide‐resin linkage. Amino acid analysis peptide‐resins before after allowed calculation...
Micrurus spixii venom was studied after fractionation by Sephadex G-100 SF gel filtration chromatography. Several enzymatic activities and biological effects were investigated in whole fractions. The resolved four peaks a range of about 73.2-10.7 kDa molecular weight. Alkaline phosphatase acetylcholinesterase found peak I, procoagulant activity seen II. Phospholipase A2, hemorrhagic, proteolytic detected III. A second factor proteinase present IV. Thrombin-like enzyme direct hemolytic not...
Se ha investigado el contenido proteico y algunas actividades enzimáticas del veneno de la serpiente cascabel Crotalus durissus terrificus procedente región Sandia, Puno; para ello se empleó total así como las fracciones obtenidas mediante cromatografía filtración en Sephadex G-100. El porcentaje proteína calculado por método Lowry fue 68,6% total; habiéndose obtenido 3 picos durante fraccionamiento; primero registró actividad proteolítica, segundo, amidolítica, coagulante fosfolipasa A2,...
Crotalphine (CRP) is a 14‐mer peptide isolated from the venom of C. durissus terrificus “rattlesnake” that triggers long‐lasting antinociception (3‐5 days). This effect mediated by activation peripheral CB2 cannabinoid receptors, subsequent release dynorphin A and opioid receptors. In inflamed tissues, granulocytes macrophages are main source peptides, which play substantial role in modulation pain via interactions with receptors located sensory nerve fibers. this work, we synthesized CRP...
Losac (a protein from Lonomia obliqua caterpillar) shows high similarity with the four first Ig‐like domains of NCAMs human which are crucial for embryonic development neural system. Previous studies known that is capable to inhibit endothelial cell death. Using a model serum deprivation, we investigated molecular mechanism by recombinant (produced in bacteria), exerts its protective effect dorsal root ganglia (DRG) neurons. Cell survival (MTT assay) was better observed after 48h treatment....
Abstract Review: 175 refs.