A5043261620- Food Allergy and Anaphylaxis Research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Insect and Pesticide Research
- Healthcare and Venom Research
- vaccines and immunoinformatics approaches
- Immune responses and vaccinations
- Bee Products Chemical Analysis
- Insect and Arachnid Ecology and Behavior
- Immune Cell Function and Interaction
- Assisted Reproductive Technology and Twin Pregnancy
- Occupational exposure and asthma
- Venomous Animal Envenomation and Studies
- interferon and immune responses
- Animal Virus Infections Studies
- Immunodeficiency and Autoimmune Disorders
- Mast cells and histamine
- COVID-19 epidemiological studies
- Entomological Studies and Ecology
- Neurobiology and Insect Physiology Research
- SARS-CoV-2 detection and testing
- Asthma and respiratory diseases
- Insect Utilization and Effects
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2019-2024
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2011-2023
Universidade de São Paulo
2005-2022
Weatherford College
2022
Instituto do Coração
2021
Associação Brasileira de Alergia e Imunologia
2019
Hospital São Paulo
2012
Universidade Estadual Paulista (Unesp)
2005-2010
Universidade Federal do Rio de Janeiro
2010
Fundação Oswaldo Cruz
2010
Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure multiple organ that can lead death. Previous attempts develop specific antivenom treat these victims have been unsuccessful. We herein report a F(ab)´2-based raised in horse as potential new treatment for stings. The final product contains high IgG titers is effective neutralizing toxic effects, such hemolysis, cytotoxicity myotoxicity. assessment neutralization...
Background Celery (Apium graveolens) represents a relevant allergen source that can elicit severe reactions in the adult population. To investigate sensitization prevalence and cross-reactivity of Api g 2 from celery stalks Mediterranean population mouse model. Methodology 786 non-randomized subjects Italy were screened for IgE reactivity to rApi 2, rArt v 3 (mugwort pollen LTP) nPru p (peach using an microarray. Clinical data 32 selected patients with LTP under investigation evaluated....
Despite the high number of individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) who develop disease 2019 (COVID-19) symptoms worldwide, many exposed remain asymptomatic and/or uninfected and seronegative. This could be explained a combination environmental (exposure), immunological (previous infection), epigenetic, genetic factors. Aiming to identify factors involved in immune response symptomatic COVID-19 as compared individuals, we analyzed 83 Brazilian...
The study reported here is a classical bottom-up proteomic approach where proteins from wasp venom were extracted and separated by 2-DE; the individual protein spots proteolytically digested subsequently identified using tandem mass spectrometry database query with search engine MASCOT. Eighty-four belonging to 12 different molecular functions identified. These classified into three groups; first constituted of typical proteins: antigens-5, hyaluronidases, phospholipases, heat shock...
Even after over 2 years of the COVID-19 pandemic, research on rapid, inexpensive, and accurate tests remains essential for controlling avoiding global spread SARS-CoV-2 across planet during a potential reappearance in future waves or regional outbreaks. Assessment serological responses can be beneficial population-level surveillance purposes, supporting development novel vaccines evaluating efficacy different immunization programs. This especially relevant broadly used inactivated whole...
CoronaVac is an inactivated SARS-CoV-2 vaccine that has been rolled out in several low and middle-income countries including Brazil, where it was the mainstay of first wave immunization healthcare workers elderly population. We aimed to assess T cell antibody responses vaccinated individuals as compared convalescent patients. detected IgG against antigens, neutralizing antibodies reference Wuhan strain used peptides detect IFN-g IL-2 specific a group (N = 101) 72) individuals. The frequency...
Introdução: O uso de células-tronco na medicina regenerativa tem emergido como uma abordagem promissora para o tratamento feridas complexas e doenças cutâneas, oferecendo novos caminhos a regeneração tecidos danificados. Objetivo: Analisar papel das cutânea, avaliando as diversas fontes células-tronco, seus mecanismos ação suas aplicações clínicas, além discutir os desafios avanços tecnológicos no campo da regenerativa. Metodologia: Foi realizada revisão bibliográfica Biblioteca Virtual em...
ABSTRACT Background Clinical recurrence of COVID-19 in convalescent patients has been reported, which immune mechanisms have not thoroughly investigated. Presence neutralizing antibodies suggests other types response are involved. Methods We assessed the innate type I/III IFN response, T cell responses to SARS-CoV-2 with IFNγ ELISPOT, binding and antibody assays, two monozygotic twin pairs one case. Results In pair 1, four months after a first mild episode infection for both siblings,...
Abstract The phospholipases A 1 (PLA s) from the venom of social wasp Polybia paulista occur as a mixture different molecular forms. To characterize origin these structural differences, an experimental strategy was planned combining isolation pool PLAs with proteomic approaches by using 2‐D, MALDI‐TOF‐TOF MS and classical protocols protein chemistry, which included N‐ C‐terminal sequencing. existence intact form PLA seven truncated forms identified, apparently originating controlled...
Honey bee venom toxins trigger immunological, physiological, and neurological responses within victims. The high occurrence of attacks involving potentially fatal toxic allergic reactions in humans the prospect developing novel pharmaceuticals make honey an attractive target for proteomic studies. Using label-free quantification, we compared proteome phosphoproteome Africanized honeybees with that two European subspecies, namely Apis mellifera ligustica A. m. carnica. From total 51 proteins,...
Introduction Considering the likely need for development of novel effective vaccines adapted to emerging relevant CoV-2 variants, increasing knowledge epitope recognition profile among convalescents and afterwards vaccinated with identification immunodominant regions may provide important information. Methods We used an RBD peptide microarray identify IgG IgA binding in serum 71 COVID-19 18 individuals. Results found a set antibody epitopes, each recognized by more than 30% tested cohort,...
Although aging correlates with a worse prognosis for Covid-19, super elderly still unvaccinated individuals presenting mild or no symptoms have been reported worldwide. Most of the genetic variants responsible increased disease susceptibility are associated immune response, involving type I IFN immunity and modulation; HLA cluster genes; inflammasome activation; genes interleukins; chemokines receptors. On other hand, little is known about resistance mechanisms against SARS-CoV-2 infection....
Sublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific with peptide vaccines far less, but it of great interest in the treatment allergic reactions, as precludes drawbacks allergen extracts. The ideal candidates would bind IgG, blocking IgE-binding. To better elucidate IgE and IgG4 epitope profiles during SLIT, sequences main allergens, Der p 1, 2, 5, 7, 10, 23 Blo t 6, 12, 13, were included a 15-mer microarray tested against pooled sera...
The Coronaviridae family comprises seven viruses known to infect humans, classified into alphacoronaviruses (HCoV-229E and HCoV-NL63) betacoronaviruses (HCoV-OC43 HCoV-HKU1), which are considered endemic. Additionally, it includes SARS-CoV (severe acute respiratory syndrome), MERS-CoV (Middle East the novel coronavirus SARS-CoV-2, responsible for COVID-19. SARS-CoV-2 induces severe complications, particularly in elderly, immunocompromised individuals those with underlying diseases. An...
Rheumatic heart disease (RHD) affects heart-valve tissue and is the most serious consequence of group A Streptococcus infection. Myxomatous degeneration (MXD) frequent valvopathy in western world. In present work, key protein expression alterations RHD MXD patients were identified characterized, with controls from cadaveric organ donors. Proteins separated by two-dimensional (2D)-electrophoresis mass spectrometry. We found 17 differentially expressed spots, as compared to control samples....
Abstract Urine is an ideal source of materials to search for potential disease‐related biomarkers as it produced by the affected tissues and can be easily obtained noninvasive methods. 2‐DE‐based proteomic approach was used better understand molecular mechanisms injury induced fluoride (F − ) define dental fluorosis. Three groups weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F 60 days ( n = 15/group). During experimental period, animals kept...
Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food chemistry industry. We aimed to recombinantly produce characterize first manioc allergen evaluate its IgE reactivity sera of Brazilian Italian patients.The molecule, termed Man e5, was expressed E. coli, characterized amino acid analysis, mass spectrometry, circular dichroism, HPLC, dynamic light scattering. A tertiary structural model protein produced using bioinformatics...
Abstract Background Although older adults are at a high risk of severe or critical Covid-19, there many cases unvaccinated centenarians who had silent infection recovered from mild moderate Covid-19. We studied three Brazilian supercentenarians, than 110 years, survived Covid-19 in 2020 before being vaccinated. Results Despite their advanced age, humoral immune response analysis showed that these individuals displayed robust levels IgG and neutralizing antibodies (NAbs) against SARS-CoV-2....
The COVID-19 pandemic has prompted a quest to understand why certain individuals remain uninfected or asymptomatic despite repetitive exposure SARS-CoV-2. Here, we focused on six exposed females residing with their symptomatic and reinfected SARS-CoV-2 PCR-positive partners. Peripheral blood mononuclear cell samples from couples were analysed for poly (I:C)-induced mRNA expression of type I/III interferons interferon-stimulated genes (ISGs). Remarkably, found significant upregulation the ISG...
Abstract Background Despite the high number of individuals infected by SARS-CoV-2 who develop COVID-19 symptoms worldwide, many exposed remain asymptomatic and/or stay uninfected. This could be explained a combination environmental (exposure, previous infection), epigenetic, and genetic factors. Aiming to identify variants involved in resistance, we analyzed 86 discordant Brazilian couples where one was symptomatic while partner remained seronegative despite sharing same bedroom during...
Post-orgasmic illness syndrome (POIS) is a rare condition characterized by post-ejaculatory symptoms. Here reported the first Brazilian POIS patient. Immunological investigation did not confirm previous hypothesis of hypersensitivity reaction. Cell immunophenotyping comparing healthy individuals produced evidence abnormalities associated to clinical manifestations. The patient was submitted specific immunotherapy with transient response and referred psychologist but demonstrate improvement...