A5071304375- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Trypanosoma species research and implications
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- Research on Leishmaniasis Studies
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Viral Infections and Vectors
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Complement system in diseases
- Viral Infections and Outbreaks Research
- Immune Response and Inflammation
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Virology and Viral Diseases
- Vibrio bacteria research studies
- Escherichia coli research studies
- Parasitic Infections and Diagnostics
- Hepatitis B Virus Studies
- Circadian rhythm and melatonin
Universidade de São Paulo
2016-2025
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2023
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2021-2022
Instituto de Medicina Avançada
2022
Instituto do Coração
2021
Rockefeller University
2006-2010
Universidade Federal de São Paulo
1999-2007
Center of Molecular Immunology (Cuba)
2006
University of Antofagasta
1999
Resistance to several prevalent infectious diseases requires both cellular and humoral immune responses. T cell immunity is initiated by mature dendritic cells (DCs) in lymphoid organs, whereas responses most antigens require further collaboration between primed, antigen-specific helper naive or memory B cells. To determine whether delivered DCs organs induce help for antibody responses, we targeted a carrier protein, ovalbumin (OVA), the presence of maturation stimulus assayed antibodies...
Presumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse human aorta. We used promoter–enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs study their antigen-presenting capacity. Stellate EYFP+ were readily aorta could be double labeled with antibodies major histocompatability complex (MHC) II products. The proved particularly abundant cardiac valves sinus. In all locations, CD11c+ localized...
Dengue is the most prevalent arboviral infection, affecting millions of people every year. Attempts to control such infection are being made, and development a vaccine World Health Organization priority. Among proteins tested as candidates in preclinical settings non-structural protein 1 (NS1). In present study, we immune responses generated by targeting NS1 two different dendritic cell populations. Dendritic cells (DCs) important antigen presenting cells, maturing DCs has proved be an...
The murine model of T. cruzi infection has provided compelling evidence that development host resistance against intracellular protozoans critically depends on the activation members Toll-like receptor (TLR) family via MyD88 adaptor molecule. However, possibility TLR/MyD88 signaling pathways also control induction immunoprotective CD8+ T cell-mediated effector functions not been investigated to date. We addressed this question by measuring frequencies IFN-gamma secreting cells specific for...
Clearing blood-stage malaria parasites without inducing major host pathology requires a finely tuned balance between pro- and anti-inflammatory responses. The interplay regulatory T (Treg) cells dendritic (DCs) is one of the key determinants this balance. Although experimental models have revealed various patterns Treg cell expansion, DC maturation, cytokine production according to infecting parasite species, no studies compared all these parameters in human infections with Plasmodium...
Dendritic cells (DCs) are phagocytes that highly specialized for antigen presentation. Heterogeneous populations of macrophages and DCs form a phagocyte network inside the red pulp (RP) spleen, which is major site control blood-borne infections such as malaria. However, dynamics splenic during Plasmodium poorly understood, limiting our knowledge regarding their protective role in Here, we used vivo experimental approaches enabled us to deplete or visualize order clarify these issues. To...
CoronaVac is an inactivated SARS-CoV-2 vaccine that has been rolled out in several low and middle-income countries including Brazil, where it was the mainstay of first wave immunization healthcare workers elderly population. We aimed to assess T cell antibody responses vaccinated individuals as compared convalescent patients. detected IgG against antigens, neutralizing antibodies reference Wuhan strain used peptides detect IFN-g IL-2 specific a group (N = 101) 72) individuals. The frequency...
Protective immunity against lethal infection is developed when BALB/c or C57BL/6 mice are immunized with plasmids containing genes from the protozoan parasite Trypanosoma cruzi. However, genetic vaccination of highly susceptible mouse strain A/Sn promoted limited survival after challenge. This observation questioned whether this type would be appropriate for individuals. Here, we compared protective efficacy and immune response individual combined encoding trans-sialidase (TS) amastigote...
The heat-labile toxins (LT) produced by enterotoxigenic Escherichia coli (ETEC) display adjuvant effects to co-administered antigens, leading enhanced production of serum antibodies. Despite extensive knowledge the properties LT derivatives, including in vitro generated non-toxic mutant forms, little is known about capacity these adjuvants modulate epitope specificity antibodies directed against antigens. present study characterizes role and its B subunit (LTB) modulation antibody responses...
ABSTRACT We previously described that DNA vaccination with the gene encoding amastigote surface protein 2 (ASP-2) protects approximately 65% of highly susceptible A/Sn mice against lethal Trypanosoma cruzi infection. Here, we explored possibility bacterial recombinant proteins ASP-2 could be used to improve efficacy vaccinations. Initially, compared protective regimens using either a plasmid DNA, protein, or both sequentially (DNA priming and boosting). Survival after challenge was not...
Immunization of mice with plasmids containing Trypanosoma cruzi genes induced specific antibodies, CD4(+) Th1 and CD8(+) Tc1 cells, protective immunity against infection. In most cases, used for DNA vaccination contained encoding antigens expressed by trypomastigotes, the nonreplicative forms parasite. this study, we explored possibility using amastigotes, form parasite which replicates inside host experimental vaccination. For that purpose, selected a gene related to amastigote surface...
Obligatory intracellular parasites such as Plasmodium sp, Trypanosoma cruzi, Toxoplasma gondii and Leishmania sp are responsible for the infection of hundreds millions individuals every year. These can deliver antigens to host cell cytoplasm that presented through MHC class I molecules protective CD8 T cells. The in vivo priming conditions specific cells during natural largely unknown remain an area has been poorly explored. antiparasitic mechanisms mediated by include both...
Salmonella flagellin, the flagellum structural subunit, has received particular interest as a vaccine adjuvant conferring enhanced immunogenity to soluble proteins or peptides, both for activation of antibody and cellular immune responses. In present study, we evaluated enterica FliCd flagellin T cell using model 9-mer (SYVPSAEQI) synthetic H2(d)-restricted CD8(+) cell-specific epitope (CS(280-288)) derived from Plasmodium yoelii circumsporozoite (CS) protein. The effects were determined...
The development of a preventive vaccine against human immunodeficiency virus (HIV-1) infection is the most efficient method to control epidemic. ultimate goal develop able induce specific neutralizing, non-neutralizing antibodies and cellular mediated immunity (CMI). Humoral CMI responses can be directed glycoproteins that are normally presented as trimeric spike on surface (gp140). Despite safer, subunit vaccines less immunogenic/effective need delivered together with an adjuvant. choice...
Dengue fever has become a global threat, causing millions of infections every year. An effective vaccine against all four serotypes dengue virus (DENV) not been developed yet. Among the different vaccination strategies available today, DNA vaccines are safe and practical, but currently induce relatively weak immune responses in humans. In order to improve immunogenicity, antigens may be targeted dendritic cells (DCs), main antigen presenting orchestrators adaptive response, inducing T B cell...
ABSTRACT Background Clinical recurrence of COVID-19 in convalescent patients has been reported, which immune mechanisms have not thoroughly investigated. Presence neutralizing antibodies suggests other types response are involved. Methods We assessed the innate type I/III IFN response, T cell responses to SARS-CoV-2 with IFNγ ELISPOT, binding and antibody assays, two monozygotic twin pairs one case. Results In pair 1, four months after a first mild episode infection for both siblings,...