A5034758961- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Yersinia bacterium, plague, ectoparasites research
- Complement system in diseases
- RNA Interference and Gene Delivery
- vaccines and immunoinformatics approaches
- Bacillus and Francisella bacterial research
- Escherichia coli research studies
- Vector-borne infectious diseases
- Migraine and Headache Studies
- Immune cells in cancer
- Malaria Research and Control
- CAR-T cell therapy research
- Mosquito-borne diseases and control
- Asthma and respiratory diseases
- Hepatitis B Virus Studies
- Cell Adhesion Molecules Research
- IL-33, ST2, and ILC Pathways
- NF-κB Signaling Pathways
- Pharmacological Effects of Natural Compounds
Yonsei University
2015-2024
Ewha Womans University
2022-2023
Rockefeller University
2007-2018
Korea Institute of Brain Science
2018
Howard Hughes Medical Institute
1993-2007
Icahn School of Medicine at Mount Sinai
2003
Armed Forces Capital Hospital
2000
Dendritic cells (DCs) process and present self foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation antigen, but little known about how DCs control antigen in vivo. To examine processing vivo, we specifically targeted two major subsets using chimeric monoclonal antibodies. Unlike CD8 + express cell surface protein CD205, – DCs, which are positive for 33D1 specialized on histocompatibility complex...
Improved protein-based vaccines should facilitate the goal of effective against HIV and other pathogens. With respect to T cells, efficiency immunization, or “immunogenicity,” is improved by targeting vaccine proteins maturing dendritic cells (DCs) within mAbs DC receptors. Here, we compared capacity Langerin/CD207, DEC205/CD205, Clec9A receptors, each expressed on CD8 + subset in mice, bring about immunization microbial-specific from polyclonal repertoire, using gag-p24 protein as an...
Current human immunodeficiency virus (HIV) vaccine approaches emphasize prime boost strategies comprising multiple doses of DNA and recombinant viral vectors. We are developing a protein-based approach that directly harnesses principles for generating T cell immunity. Vaccine is delivered to maturing dendritic cells in lymphoid tissue by engineering protein antigen into an antibody DEC-205, receptor presentation. Here we characterize the CD4+ immune response HIV gag compare efficacy with...
Presumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse human aorta. We used promoter–enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs study their antigen-presenting capacity. Stellate EYFP+ were readily aorta could be double labeled with antibodies major histocompatability complex (MHC) II products. The proved particularly abundant cardiac valves sinus. In all locations, CD11c+ localized...
Abstract The C-type lectin dendritic cell-specific ICAM 3-grabbing nonintegrin (DC-SIGN)/CD209 efficiently binds several pathogens, including HIV-1. DC-SIGN is expressed on monocyte-derived DCs in culture, and importantly, it able to sequester HIV-1 within cells facilitate transmission of virus CD4+ T cells. To investigate function, we have generated new mAbs. We report this study that these prior anti-DC-SIGN mAbs primarily label macrophages the medullary sinuses noninflamed human lymph...
SIGN-R1, a recently discovered C-type lectin expressed at high levels on macrophages within the marginal zone of spleen, mediates uptake dextran polysaccharides by these phagocytes. We now find that encapsulated Streptococcus pneumoniae are rapidly cleared from bloodstream, and capture also takes place when different cell lines express SIGN-R1 after transfection. To assess role capsular polysaccharide S. (CPS) in interaction with pneumococci, we first studied binding serotype 14 CPS...
DNA vaccines promote an immune response by providing antigen-encoding to the recipient, but efficacy of such needs improving. Many approaches have considerable potential currently induce relatively weak responses despite multiple high doses vaccine. Here, we asked whether targeting vaccine antigens DCs would increase immunity and protection that result from vaccines. To determine this, generated a encoding fusion protein comprised antigen single-chain Fv antibody (scFv) specific for...
Dendritic cells (DCs) are strategically positioned to take up antigens and initiate adaptive immunity. One DC subset expresses CD8alphaalpha in mice is specialized capture dying process for MHC class I "cross-presentation." Because CD8(+) DCs also express DEC205/CD205, which localized splenic T cell regions, it thought that restricted zones. Here, we used a new antibody Langerin/CD207, colabels isolated CD205(+) DCs, immunolabel spleen sections. The mAb labeled discrete with high levels of...
Abstract The targeted delivery of Ags to dendritic cell (DCs) in vivo greatly improves the efficiency Ag presentation T cells and allows an analysis receptor function. To evaluate function Langerin/CD207, a expressed by subsets DCs that frequently coexpress DEC205/CD205 receptor, we genetically introduced OVA into C terminus anti-receptor Ab H chains. Taking advantage new L31 mAb extracellular domain mouse Langerin, find hybrid targets appropriate DC draining lymph nodes spleen. is then...
Protein vaccines, if rendered immunogenic, would facilitate vaccine development against HIV and other pathogens. We compared in nonhuman primates (NHPs) immune responses to Gag p24 within 3G9 antibody DEC205 (“DEC-HIV p24”), an uptake receptor on dendritic cells, nontargeted protein, with or without poly ICLC, a synthetic double stranded RNA, as adjuvant. Priming s.c. 60 μg of both vaccines elicited potent CD4 + T cells secreting IL-2, IFN-γ, TNF-α, which also proliferated. The increased...
DC-SIGN, a human C-type lectin, is expressed on the surface of dendritic cells (DC), while closely related gene, DC-SIGNR or L-SIGN, found sinusoidal endothelial liver and lymph node. Both DC-SIGN DC-SIGNR/L-SIGN can bind ICAM-3 HIV gp120, transmit to susceptible in trans. Here, we report cloning five mouse genes homologous DC-SIGNR/L-SIGN. Only one named highly DC, not panel macrophage lymphocyte cell lines. The other four genes, SIGNR1 (SIGN-Related gene 1), SIGNR2, SIGNR3 SIGNR4, are at...
The marginal zone macrophages of the spleen are implicated in clearance polysaccharides, but underlying mechanisms need to be pinpointed. SIGN‐R1 is one five recently identified mouse genes that homologous human DC‐SIGN and encode a single, external, C‐terminal C‐type lectin domain. We find polyclonal antibody specific peptide reacts primarily strongly with subset lymph node medulla. In both sites, exists an aggregated form, resistant dissociation into monomers upon boiling SDS under...
CD30 is a member of the tumor necrosis factor superfamily and surface marker for Hodgkin's disease. Normal activated T cells several virally transformed or B cell lines also show expression. The interaction with its ligand induces death proliferation, depending on type. In this report we characterize signals mediated by intracellular domain that, in combination signal(s) transduced receptor, multimerization cytoplasmic Fas(CD95)-independent hybridomas. Deletion analysis shows that...
The mouse (m) DC-SIGN family consists of several homologous type II transmembrane proteins located in close proximity on chromosome 8 and having a single carboxyl terminal carbohydrate recognition domain. We first used transfected non-macrophage cell lines to compare the polysaccharide microbial uptake capacities three these lectins--DC-SIGN, SIGNR1 SIGNR3--to another homologue mLangerin. Each molecule shares potential mannose-recognition EPN-motif its Using an anti-Tag antibody follow...
DCs are critical for initiating immunity. The current paradigm in vaccine biology is that migrating from peripheral tissue and classical lymphoid-resident (cDCs) cooperate the draining LNs to initiate priming proliferation of T cells. Here, we observe subcutaneous immunity Fms-like tyrosine kinase 3 ligand (Flt3L) dependent. Flt3L rapidly secreted after immunization; Flt3 deletion reduces cell responses by 50%. enhances global humoral as well both numbers antigen capture capacity migratory...
Abstract Dendritic cells are antigen-presenting orchestrating innate and adaptive immunity. The crucial role of transcription factors histone modifications in the transcriptional regulation dendritic has been extensively studied. However, it is not well understood whether how three-dimensional chromatin folding controls gene expression cells. Here we demonstrate that activation bone marrow-derived induces extensive reprogramming looping as enhancer activity, both which implicated dynamic...