A5035775983- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Photoacoustic and Ultrasonic Imaging
- Glioma Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
- Long-Term Effects of COVID-19
- Cell death mechanisms and regulation
- Biosensors and Analytical Detection
- IL-33, ST2, and ILC Pathways
- Extracellular vesicles in disease
- Virus-based gene therapy research
- Phagocytosis and Immune Regulation
- COVID-19 Clinical Research Studies
- Analytical Chemistry and Sensors
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroinflammation and Neurodegeneration Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Inflammasome and immune disorders
- Monoclonal and Polyclonal Antibodies Research
Technical University of Munich
2016-2025
Institute of Medical Microbiology and Hygiene
2015-2024
Marymount University
2022
German Center for Infection Research
2020-2021
Yonsei University
2007
Howard Hughes Medical Institute
2007
Rockefeller University
2007
Dendritic cells (DCs) process and present self foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation antigen, but little known about how DCs control antigen in vivo. To examine processing vivo, we specifically targeted two major subsets using chimeric monoclonal antibodies. Unlike CD8 + express cell surface protein CD205, – DCs, which are positive for 33D1 specialized on histocompatibility complex...
A core feature of protective T cell responses to infection is the robust expansion and diversification naïve antigen-specific populations into short-lived effector long-lived memory subsets. By means in vivo fate mapping, we found a striking variability immune derived from individual CD8(+) cells show that acute recall immunity requires initial recruitment multiple precursors. Unbiased mathematical modeling identifies random integration differentiation division events as driving force behind...
CD8+ T cells that respond to chronic viral infections or cancer are characterized by the expression of inhibitory receptors such as programmed cell death protein 1 (PD-1) and impaired production cytokines. This state restrained functionality-which is referred exhaustion1,2-is maintained precursors exhausted (TPEX) express transcription factor (TCF1), self-renew give rise TCF1- effector cells3-6. Here we show long-term proliferative potential, multipotency repopulation capacity during...
Type 1 conventional dendritic cells (cDC1s) are critical for anti-cancer immunity. Protective immunity is thought to require cDC1s sustain T cell responses within tumors, but it poorly understood how this function regulated and whether its subversion contributes immune evasion. Here, we show that tumor-derived prostaglandin E2 (PGE2) programmed a dysfunctional state in intratumoral cDC1s, disabling their ability locally orchestrate CD8+ responses. Mechanistically, cAMP signaling downstream...
Type 1 conventional dendritic cells (cDC1) can support T cell responses within tumors but whether this determines protective versus ineffective anti-cancer immunity is poorly understood. Here, we use imaging-based deep learning to identify intratumoral cDC1-CD8+ clustering as a unique feature of immunity. These clusters form selectively in stromal tumor regions and constitute niches which cDC1 activate TCF1+ stem-like CD8+ cells. We distinct population immunostimulatory CCR7neg that produce...
Abstract Cancer-specific TCF1 + stem-like CD8 T cells can drive protective anticancer immunity through expansion and effector cell differentiation 1–4 ; however, this response is dysfunctional in tumours. Current cancer immunotherapies 2,5–9 promote responses some but not all patients. This variation points towards currently ill-defined mechanisms that limit cell-mediated immunity. Here we demonstrate tumour-derived prostaglandin E2 (PGE 2 ) restricts the proliferative of within tumours,...
Neuromyelitis optica is a paradigmatic autoimmune disease of the central nervous system, in which water-channel protein AQP4 target antigen
Tissue-resident memory CD8+ T cells (TRM) constitute a major component of the immune-surveillance system in nonlymphoid organs. Local, noncognate factors are both necessary and sufficient to support programming TRM cell fate tissue-infiltrating cells. Recent evidence suggests that TCR signals received infected tissues additionally contribute formation. Here, we asked how antigen-dependent pathways influence generation skin-resident arise from polyclonal repertoire induced by infection with...
T cells expressing anti-CD19 chimeric antigen receptors (CARs) demonstrate impressive efficacy in the treatment of systemic B cell malignancies, including lymphoma. However, their effect on primary central nervous system lymphoma (PCNSL) is unknown. Additionally, detailed cellular dynamics CAR during antitumor reaction remain unclear, intratumoral infiltration depth, mobility, and persistence. Studying these processes detail requires repeated intravital imaging precisely defined tumor...
Upon viral infection, natural killer (NK) cells expressing certain germline-encoded receptors are selected, expanded, and maintained in an adaptive-like manner. Currently, these thought to differentiate along a common pathway. However, by fate mapping of single NK upon murine cytomegalovirus (MCMV) we identified two distinct cell lineages that contributed responses. One was equivalent conventional (cNK) while the other transcriptionally similar type 1 innate lymphoid (ILC1s). ILC1-like...
Abstract Dendritic cells (DCs) are central modulators of immune responses and, therefore, interesting target for the induction antitumor responses. Ag delivery to select DC subpopulations via targeting Abs inhibitory receptor 2 (DCIR2, clone 33D1) or DEC205 was shown direct Ags specifically CD11c+CD8− CD11c+CD8+ DCs, respectively, in vivo. In contrast increasing knowledge about by efficiently cross-presenting little is known functional role Ag-presenting DCs with regard initiation protective...
Abstract While antigen-primed T cells proliferate at speeds close to the physiologic maximum of mammalian cells, cell memory is maintained in absence antigen by rare divisions. The transition between these distinct proliferative programs has been difficult resolve via population-based analyses. Here, we computationally reconstruct history single CD8 + upon vaccination and measure division speed emerging subsets vivo. We find that slower cycling central precursors, characterized an elongated...
To what extent the lineage decisions of activated CD4+ T cells are determined by quality cell receptor (TCR) ligation is incompletely understood. Here, we show that individual expressing identical TCRs take highly variable fate despite binding same ligand. We identify a mathematical model correctly captures this probabilistic behavior and allows one to formalize changes in TCR signal quality—due cognate versus altered peptide ligation—as lineage-specific proliferation differentiation rates....
Lung cancer patients are at risk for brain metastases and often succumb to their intracranial disease. Chimeric Antigen Receptor (CAR) T-cells emerged as a powerful cell-based immunotherapy hematological malignancies; however, it remains unclear whether CAR represent viable therapy metastases. Here, we established syngeneic orthotopic cerebral metastasis model in mice by combining chronic cranial window with repetitive intracerebral two-photon laser scanning-microscopy. This approach enabled...