A5075478234- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- Virus-based gene therapy research
- Immune Response and Inflammation
- Nanowire Synthesis and Applications
- vaccines and immunoinformatics approaches
- Escherichia coli research studies
- Blood groups and transfusion
- Cutaneous Melanoma Detection and Management
- Microfluidic and Bio-sensing Technologies
- SARS-CoV-2 and COVID-19 Research
- Cell Adhesion Molecules Research
- Rice Cultivation and Yield Improvement
- Immune responses and vaccinations
- Crop Yield and Soil Fertility
- Biosimilars and Bioanalytical Methods
- Hematopoietic Stem Cell Transplantation
- Soil Carbon and Nitrogen Dynamics
Juno Therapeutics (Germany)
2015-2024
Bristol-Myers Squibb (Germany)
2020-2024
Institute for Advanced Study
2012-2017
Focus (Germany)
2014-2017
Institute of Medical Microbiology and Hygiene
2006-2016
Technical University of Munich
2006-2016
University of Milan
2005-2011
Cambridge University Press
2011
Helmholtz Zentrum München
2009
Institute for Environment and Human Security
2007
Three distinct subsets of antigen-experienced CD8(+) T cells have been identified so far: short-living effector (T(EC)) and two long-living subsets, described as central (T(CM)) memory (T(EM)) cells. The lineage relationships these subpopulations well their involvement in protection not yet conclusively determined. We recently a novel marker combination (CD127 CD62L) to identify all three major cell mice infected with Listeria monocytogenes (L.m.). Extensive relationship analyses on highly...
A general obstacle for clinical cell preparations is limited purity, which causes variability in the quality and potency of products might be responsible negative side effects due to unwanted contaminants. Highly pure populations can obtained best using positive selection techniques. However, many cases target need segregated from other cells by combinations multiple markers, still difficult achieve--especially products. Therefore, we have generated low-affinity antibody-derived...
Abstract High epitope‐specific sensitivity of CD8 + T cells is required for optimal immune protection against intracellular pathogens as well certain malignancies. The quality antigen recognition usually described “avidity” to its cognate peptide MHCI complex. cell avidity mainly dependent on the structural qualities receptor (TCR), convincingly demonstrated by recombinant TCR re‐expression experiments. Based reversible multimer staining and k off ‐rate measurements monomeric complexes, we...
T cell activation is a cornerstone in manufacturing of cell-based therapies, and precise control over important the development next generation T-cell based therapeutics. This need cannot be fulfilled by currently available methods for stimulation, particular not time dependent manner. Here, we describe modular reagent called Expamers, which addresses these limitations. Expamers are versatile stimuli that intended research clinical use. They readily soluble can rapidly bound removed from...
Abstract CAR T cell therapy is a rapidly growing area of oncological treatments having potential becoming standard care for multiple indications. Coincidently, CRISPR/Cas gene-editing technology entering next-generation product manufacturing with the promise more precise and controllable modification methodology. The intersection these medical molecular advancements creates an opportunity completely new ways designing engineered cells to help overcome current limitations therapy. In this...
The size of the adaptive immune system is considered to be kept constant by attrition pre-existing memory. However, recently it was shown that CD8 memory compartment can grow in and number largely preserved, predicting immunity should maintained (Vezys et al.; Nature 457: 196-199). Experimental proof for this assumption still lacking. We address question Listeria monocytogenes (L.m.) infection model confirm growth subsequent vaccination with modified vaccinia virus Ankara. also find only...
Immunotherapy using TCR and especially CAR transgenic T cells is a rapidly advancing field with the potential to become standard of care for treatment multiple diseases. While all current FDA approved cell products are generated lentiviral gene transfer, extensive work put into CRISPR/Cas mediated delivery develop next generation safer more potent products. One limitation editing systems size restriction knock-in cargo. Targeted integration under control an endogenous promotor and/or...
The activating receptor NKG2D recognizes a wide range of different ligands, some which are primarily expressed in “stressed” tissues or on tumor cells. Until now, similar stimulatory effects natural killer and CD8 + T cells have been described for all the receptor/ligand system has therefore interpreted as sensor involved immune surveillance activation responses. We show here that ligands H60 MIC class 1 chain-related protein A (MICA) can also mediate strong suppressive cell proliferation....
SUMMARY A reliable evaluation of crop nutritional status is crucial for supporting fertilization aiming at maximizing qualitative and quantitative aspects production reducing the environmental impact cropping systems. Most available simulation models evaluate according to nitrogen (N) dilution law, which derives critical N concentration as a function above-ground biomass. An alternative approach, developed during project carried out with students Cropping Systems Masters course University...
Abstract The transfer of regulatory T cells, either freshly isolated, or modified, represents a promising therapeutic approach to dampen misdirected immune responses, like autoimmune diseases, chronic inflammatory syndromes and graft versus host disease. Clinical isolation highly pure cell (Treg) populations is still challenging labeling reagents can influence their viability functionality, potentially altering the potency isolated Treg products. Here we show that reversible Fab...
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared established cell enrichment protocols, affinity of cells is independent erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment good yields, high purities, and excellent functionality cells.
Abstract Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized therapy in immuno-oncology. Use high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells current standard applied this purpose. Here, we present transformation previously described technology — non-magnetic immunoaffinity column chromatography-based selection with reversible reagents into a new...
Adoptive T cell therapy (ACT) has become a treatment option for viral reactivations in patients undergoing allogeneic hematopoietic stem transplantation (alloHSCT). Animal models have shown that pathogen-specific central memory cells (TCM) are protective even at low numbers and show long-term survival, extensive proliferation high plasticity after adoptive transfer. Concomitantly, our own recent clinical data demonstrate minimal doses of purified (not in-vitro- expanded) human CMV...