A5069872962- Immunotherapy and Immune Responses
- Educational Systems and Policies
- Education and Learning Interventions
- T-cell and B-cell Immunology
- Psychosocial Factors Impacting Youth
- Immune Cell Function and Interaction
- Diverse Approaches in Healthcare and Education Studies
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Nutrition, Health and Food Behavior
- Diverse Topics in Contemporary Research
- Complement system in diseases
- vaccines and immunoinformatics approaches
- Energy and Environmental Systems
- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Healthcare Education and Workforce Issues
- Cell Adhesion Molecules Research
- HIV Research and Treatment
- Vector-borne infectious diseases
- Educational Research and Pedagogy
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Proteoglycans and glycosaminoglycans research
Rockefeller University
2006-2016
Ulsan National Institute of Science and Technology
2010-2016
Tohoku University
2010
Osaka University
2010
Virginia Commonwealth University Medical Center
2001-2004
Seoul National University
1999
Presumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse human aorta. We used promoter–enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs study their antigen-presenting capacity. Stellate EYFP+ were readily aorta could be double labeled with antibodies major histocompatability complex (MHC) II products. The proved particularly abundant cardiac valves sinus. In all locations, CD11c+ localized...
Abstract The precise role of cannabinoid receptors (CB)1 and CB2, as well endogenous ligands for these receptors, on immune cells remains unclear. In the current study, we examined effect exogenous cannabinoids murine bone marrow-derived dendritic (DCs). Addition Δ9-tetrahydrocannabinol (THC), a major psychoactive component found in marijuana or anandamide, an cannabinoid, to DC cultures induced apoptosis DCs. DCs expressed CB1 CB2 engagement both was necessary trigger apoptosis. Treatment...
Developing efficacious vaccines against enteric diseases is a global challenge that requires better understanding of cellular recruitment dynamics at the mucosal surfaces. The current paradigm T cell homing to gastrointestinal (GI) tract involves induction α4β7 and CCR9 by Peyer’s patch mesenteric lymph node (MLN) dendritic cells (DCs) in retinoic acid–dependent manner. This paradigm, however, cannot be reconciled with reports GI responses after intranasal (i.n.) delivery antigens do not...
In cancer immunotherapy, robust and efficient activation of cytotoxic CD8(+) T cell immune responses is a promising, but challenging task. Dendritic cells (DCs) are well-known professional antigen presenting that initiate regulate antigen-specific kill their target directly as well secrete IFN-γ, cytokine critical in tumor rejection. Here, we employed recently established protein cage nanoparticles, encapsulin (Encap), antigenic peptide nanocarriers by genetically incorporating the OT-1...
Recent studies on T follicular helper (Tfh) cells have significantly advanced our understanding of cell-dependent B cell responses. However, little is known about the early stage Tfh commitment by dendritic (DCs), particularly conventional CD8α+ and CD8α− DC subsets. We show that DCs localized at interfollicular zone play a pivotal role in induction antigen-specific upregulating expression Icosl Ox40l through non-canonical NF-κB signaling pathway. induced function as true helpers, resulting...
Abstract Administration of Con A induces severe injury to hepatocytes in mice and is considered be a model for human hepatitis. In the current study, we investigated role CD44 A-induced Intravenous administration (20 mg/kg) caused 100% mortality C57BL/6 CD44-knockout (KO) mice, although it was not lethal wild-type (WT) mice. lower doses (12 mg/kg body weight) into WT induced hepatitis as evident from increased plasma aspartate aminotransferase levels accompanied by active infiltration...
Abstract To help design needed new vaccines for pneumonic plague, we targeted the Yersinia pestis LcrV protein directly to CD8α + DEC‐205 or − DCIR2 DC along with a clinically feasible adjuvant, poly IC. By studying Y. in mice, could evaluate capacity of this targeting approach protect against human pathogen. The DEC‐targeted induced polarized Th1 immunity, whereas DCIR2‐targeted fewer CD4 T cells secreting IFN‐γ, but higher IL‐4, IL‐5, IL‐10, and IL‐13 production. DCIR‐2 elicited anti‐LcrV...
Purpose: Protein cages are promising nanoplatform candidates for efficient delivery systems due to their homogenous size and structure with high biocompatibility biodegradability.In this study, we investigate the potential of lumazine synthase protein cage as an antigen system dendritic cells (DCs), which induce antigen-specific T cell proliferation.Materials Methods: Ovalbumin (OVA) peptides OT-1 (SIINFEKL) OT-2 (ISQAVHAA-HAEINEAGR) were genetically inserted each was overexpressed in...
There is a need for more efficient vaccine against the bacterium Yersinia pestis, agent of pneumonic plague. The F1-LcrV (F1-V) subunit in alhydrogel known to induce humoral immunity. In this study, we utilized DC investigate cellular We genetically engineered LcrV virulence protein into anti-DEC-205/CD205 mAb and thereby targeted conjugated directly mouse DEC-205(+) situ. observed antigen-specific CD4(+) T cell immunity measured by intracellular staining IFN-gamma three different strains...
Damage-associated molecular patterns released from damaged kidney cells initiate postischemic inflammation, an essential step in the progression of ischemia-reperfusion injury (IRI). However, mechanism that coordinates this highly specific process ischemic kidneys remains to be clarified. Previously, we demonstrated CD137 NK specifically stimulates ligand (CD137L) on tubular epithelial (TECs) such TECs produced high CXCR2 chemokine levels required for neutrophil chemotaxis. We report present...
In the current study, role played by hyaluronic acid (HA) and its receptor CD44 on activation functions of dendritic cells (DCs) was investigated. Activation DCs with HA enhanced their ability to stimulate allogeneic antigen (Ag)-specific T markedly. treatment upregulated expression costimulatory molecules such as CD40, CD80, CD86 DCs. Cell mixing experiments using DC or from wild-type knockout mice well blocking studies anti-CD44 monoclonal antibodies revealed that but not a critical in...
In the current study we investigated mechanism by which β-estradiol-17-valerate (E2) induces apoptosis in T cells. To this end, C57BL/6 wild-type (+/+), Fas-deficient (C57BL/6-lpr/lpr), and FasL-deficient (C57BL/6-gld/gld) mice were treated with various concentrations of E2, including 75, 25, 5, 1, or 0.1 mg/kg body weight vehicle. The thymi from these harvested on days 4, 7 following treatment, cellularity determined. Treatment E2 caused a decrease thymic at all doses except three groups...
Abstract Breast cancer remains as a challenging disease with high mortality in women. Increasing evidence points the importance of understanding crosstalk between breast cancers and immune cells, but little is known about effect cancer-derived factors on migratory properties dendritic cells (DCs) their consequent capability inducing T cell responses. Utilizing unique 3D microfluidic device, we here showed that (MCF-7, MDA-MB-231, MDA-MB-436 SK-BR-3)-derived soluble increase migration DCs...
T follicular helper (Tfh) cells, a true B cell helper, have critical role in enhancing humoral immune responses. However, the initial differentiation of Tfh cells by dendritic (DCs), most potent antigen presenting has not been clearly understood, particularly knowledge two major conventional subsets, CD8α(+) DCs or CD8α(-) DCs. Here we demonstrated that localization marginal zone (MZ) bridging channels is closely associated with induction CXCR5(+)CCR7(low) cells. We also showed source IL-6...