We describe a simple and versatile method for bonding thermoplastics to elastomeric polydimethylsiloxane (PDMS) at room temperature. The of various including polycarbonate (PC), cyclic olefin copolymer (COC), polymethylmethacrylate (PMMA), polystyrene (PS), PDMS has been demonstrated An irreversible was formed instantaneously when the thermoplastics, activated by oxygen plasma followed aminopropyltriethoxysilane modification, were brought into contact with treated PDMS. surface modified...

This paper presents a cost-effective, rapid, and fully automated lab-on-a-disc for simultaneous detection of multiple protein biomarkers in raw samples such as whole blood or saliva. For the diagnosis cardiovascular disease, here, novel centrifugal microfluidic layout was designed to conduct high sensitivity C-reactive protein, cardiac troponin I, N-terminal pro-B type natriuretic peptide based on bead-based sandwich enzyme-linked immunosorbent assay (ELISA). Three reaction chambers are...

This paper describes a new label-free cell separation method using magnetic repulsion force resulting from the susceptibility difference between cells and paramagnetic buffer solution in microchannel. The forces acting on different-sized is enhanced by adjusting of surrounding medium, which depends concentration salts, such as biocompatible gadolinium diethylenetriamine pentaacetic acid (Gd–DTPA), dissolved therein. As proof-of-concept demonstration, Gd–DTPA solutions at concentrations 0–80...

Size-based filtration techniques have been developed for high-throughput isolation of extracellular vesicles (EVs). Conventional direct systems limitations in that large particles generally not only block the pores membrane but also damage because high fluid pressure. Here, we propose a cyclic tangential flow (TFF) system includes two membranes with pore sizes 200 and 30 nm, connected to peristaltic pump feeds stream flowing continuous circulation. The TFF is better able isolate specific...

This paper reports a lens-integrated liquid crystal display (LCD)-based optoelectronic tweezers (OET) system for interactive manipulation of polystyrene microspheres and blood cells by optically induced dielectrophoretic force. When dynamic image pattern is projected into specific area photoconductive layer in an OET, virtual electrodes are generated spatially resolved illumination the layer, resulting dielectrophoresis microparticles suspended under nonuniform electric field. In this study,...

We demonstrate rapid manipulation and selective concentration of microparticles using AC electrokinetics such as dielectrophoresis (DEP) electro-osmosis (ACEO) in an optoelectrofluidic platform based on a liquid crystal display (LCD). When 10 V bias at kHz was applied to the device, only 1 microm-diameter polystyrene particles were concentrated into projected LCD image patterns closely packed, forming crystalline structure by ACEO flow, while 6 repelled negative DEP forces. have...

We present a method to discriminate normal oocytes in an optoelectrofluidic platform based on the optically induced positive dielectrophoresis (DEP) for vitro fertilization. By combining gravity with pulling-up DEP force that is by dynamic image projected from liquid crystal display, discrimination performance could be enhanced due reduction friction acting are relatively large and heavy cells being affected field. The voltage condition of 10 V bias at 1 MHz was applied moving oocytes....

In this study, we describe a novel platform based on centrifugal microfluidics for simultaneous determination of nitrite, nitrate and ammonium, orthophosphate, silicate in water samples. All processes from sample metering to detection were integrated automatically conducted rotating disc-shaped device. Fluid transfer was controlled by laser irradiation the ferrowax-based microvalves. Liquid samples reagents pumped force disc, their positions movements through programmable light diode. This...

A new automated microfluidic platform can deliver a wide range of mechanical stimuli for functional neural imaging in<italic>C. elegans</italic>.

A sandwich immunoassay is a powerful tool for identifying specific substance in biological sample. However, its heterogeneous strategy always requires repetitive liquid handlings and long processing time. Here an optoelectrofluidic platform simple, fast, automated detection of human tumor marker based on surface-enhanced Raman scattering (SERS) has been developed. By using conventional device crystal display module, simple quantitative marker, alpha-fetoprotein, ∼500 nL sample droplet...

A novel active surface-enhanced Raman scattering (SERS) platform for dynamic on-demand generation of SERS sites based on optoelectrofluidics is presented in this paper. When a laser source projected into sample solution containing metal nanoparticles an optoelectrofluidic device and alternating current (ac) electric field applied, the are spontaneously concentrated assembled within spot, form SERS-active sites, enhance signal significantly, allowing more sensitive detection. In simple...

This paper describes the active control of capillary-driven flow in using a centrifugal device.

The sarcomere, the fundamental unit of muscle contraction, is a highly-ordered complex hundreds proteins. Despite decades genetics work, functional relationships and roles those sarcomeric proteins in animal behaviors remain unclear. In this paper, we demonstrate that optogenetic activation motor neurons induce contraction can facilitate quantitative studies kinetics C. elegans. To increase throughput study, trapped multiple worms parallel microfluidic device illuminated for photoactivation...

This paper describes a grayscale optoelectronic tweezers (OET) which allows adjustment of the electric field strength at each position OET. A light image was used to pattern vertical on an As depends brightness point, brighter patterns generate stronger in Its feasibility for application cell manipulation demonstrated by aligning highly motile protozoan cells direction. Depending pixel, behaviors aligned varied due different cell.

We present elastomeric membrane valves integrated into a centrifugal microfluidic platform for precise control of fluid on disc. The amount the passing through valves, which depends rotating speed disc and thickness, has been characterized, could be precisely controlled by tuning motion.

This paper describes a novel selective immobilization technique based on optical control of the sol–gel transition thermoreversible Pluronic gel, which provides simple, versatile, and biocompatible approach for high-resolution imaging microinjection Caenorhabditis elegans.