A5050629514- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- Vibrio bacteria research studies
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- HIV Research and Treatment
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Probiotics and Fermented Foods
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Food Chemistry and Fat Analysis
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
Universidade de São Paulo
2015-2023
Universidade Estadual de Santa Cruz
2017
Abstract Diagnosing Zika virus (ZIKV) infections has been challenging due to the cross-reactivity of induced antibodies with other flavivirus. The concomitant occurrence ZIKV and Dengue (DENV) in endemic regions requires diagnostic tools ability distinguish these two viral infections. Recent studies demonstrated that immunoassays using C-terminal fragment NS1 antigen (ΔNS1) can be used discriminate from DENV In order serological tests, expression/solubility ΔNS1 growth recombinant E. coli...
Malignant brain tumors are among the most aggressive cancers with poor prognosis and no effective treatment. Recently, we reported oncolytic potential of Zika virus infecting destroying human central nervous system (CNS) in vitro immunodeficient mice model. However, translating this approach to humans requires pre-clinical trials another immunocompetent animal Here, analyzed safety Brazilian (ZIKVBR) intrathecal injections three dogs bearing spontaneous CNS aiming an anti-tumoral therapy. We...
The heat-labile toxins (LT) produced by enterotoxigenic Escherichia coli (ETEC) display adjuvant effects to co-administered antigens, leading enhanced production of serum antibodies. Despite extensive knowledge the properties LT derivatives, including in vitro generated non-toxic mutant forms, little is known about capacity these adjuvants modulate epitope specificity antibodies directed against antigens. present study characterizes role and its B subunit (LTB) modulation antibody responses...
We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for periods ranging from 78 to 298 days post symptom onset. The patients were evaluated regarding different body fluids (blood, saliva, urine semen), development immune responses (including antibodies, cytokines chemokines), genetic variation within samples collected semen during infection course. analysis was focused primarily on male who shed up 158 after initial symptoms. ZIKV particles detected...
Dengue fever is caused by any of the four known dengue virus serotypes (DENV1 to DENV4) that affect millions people worldwide, causing a significant number deaths. There are vaccines based on chimeric viruses, but they still not in clinical use. Anti-DENV vaccine strategies nonstructural proteins promising alternatives those whole or structural proteins. The DENV protein 5 (NS5) main target anti-DENV T cell-based immune responses humans. In this study, we purified soluble recombinant form...
The use of intestinal probiotic bacteria is very common in the food industry and has been focus majority research this field. Yet recent years, on extraintestinal microorganisms greatly increased due to their well-known potential as probiotics. Thus, we studied a strain Lactobacillus fermentum (TCUESC01) extracted from fermenting cocoa. First, examined impact pH growth its survival under conditions similar those human gastrointestinal tract. L. TCUESC01 demonstrated resistance mimicking...
The aim of this study was to achieve greater specificity dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (ΔNS1) with regard cross-reactive Zika (ZIKV) anti-NS1 antibody responses. This is relevance in endemic regions for the discrimination both DENV and ZIKV, such as Brazil other tropical countries. ΔNS1 proteins were obtained antigens evaluated solid-phase-bound ELISA test detect IgG antibodies. performance ∆NS1-based assessed mouse...
In this study we evaluated the association of high hydrostatic pressure (HHP) and alkaline pH as a minimally denaturing condition for solubilization inclusion bodies (IBs) generated by recombinant proteins expressed Escherichia coli strains. The method was successfully applied to form dengue virus (DENV) non-structural protein 1 (NS1). minimal IBs at bar 12 while 10 sufficient HHP: 2.4 kbar 90 min 0.4 14 h 30 min. An optimal refolding achieved compression HHP 10.5 in presence arginine,...
Dengue nonstructural protein 1 (NS1) is a multi-functional glycoprotein with essential functions both in viral replication and modulation of host innate immune responses. NS1 has been established as good surrogate marker for infection. In the present study, we generated four anti-NS1 monoclonal antibodies against recombinant from dengue virus serotype 2 (DENV2), which were used to map three epitopes. The sequence 193AVHADMGYWIESALNDT209 was recognized by 2H5 4H1BC, also cross-reacted Zika...
Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these Removal of the chaotropes frequently causes reaggregation poor recovery bioactive proteins. High hydrostatic pressure (HHP) alkaline pH two conditions that, presence low level chaotropes, have been described as non-denaturing agents. In study we evaluated strategy combination...
In the present study we evaluated features of different recombinant forms Zika virus (ZIKV) proteins produced in either bacterial (Eschericha coli) or insect cells (Drosophila melanogaster). The ZIKV-envelope glycoprotein (EZIKV) is responsible for entry into host cells, main target neutralizing antibodies and has been used as a antigen serological tests development subunit vaccines. EZIKV composed three structural functional domains (EDI, EDII, EDIII), which share extensive sequence...
This study was performed to determine whether Dengue virus (DENV) immunochromatographic tests can detect and differentiate nonstructural protein 1 (NS1) from each of the four DENV serotypes do not cross-react with Zika (ZIKV) NS1 protein.We compared specificity six NS1-based (point care) in detection proteins ZIKV. The were produced mammalian cells. Cross-reactivity confirmed a purified recombinant ZIKV DENV+ or ZIKV+ human serum samples.Cross-reaction observed 2 out 6 evaluated using cell...
Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected neurological disorders. Serological diagnosis ZIKV infection still unmet challenge due to the co-circulation dengue virus, which shares extensive sequence conservation structural proteins leading generation cross-reactive antibodies. In this study, we aimed obtain tools for development improved...
Severe dengue virus (DENV) infection is characterized by exacerbated inflammatory responses that lead to endothelial dysfunction and plasma leakage. We have recently demonstrated Toll-like receptor 2 (TLR2) on blood monocytes senses DENV leading activation. Here, we report non-infectious immature particles, which are released in large numbers DENV-infected cells, drive activation via the TLR2 axis. show fully particles induce a rapid, within 6 hours post-infection, response PBMCs....
The C-terminal portion of the E protein, known as stem, is conserved among flaviviruses and an important target to peptide-based antiviral strategies. Since dengue (DENV) Zika (ZIKV) viruses share sequences in stem region, this study we evaluated cross-inhibition ZIKV by stem-based DV2 peptide (419–447), which was previously described inhibit all DENV serotypes. Thus, anti-ZIKV effects induced treatments with were tested both vitro vivo conditions. Molecular modeling approaches have...
In the present study, we evaluated immunological responses induced by dengue vaccines under experimental conditions after delivery via a transcutaneous (TC) route. Vaccines against type 2 Dengue virus particles (DENV2 New Guinea C (NGC) strain) combined with enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) were administered to BALB/c mice in three-dose immunization regimen TC As control for parenteral administration route, other mouse groups immunized same vaccine formulation...
Zika virus (ZIKV) is a globally-distributed flavivirus transmitted to humans by Aedes mosquitoes, usually causing mild symptoms that may evolve severe conditions, including neurological alterations, such as neonatal microcephaly and Guillain-Barré syndrome. Due the absence of specific effective preventive methods, we designed new subunit vaccine based on DNA vector (pgDNS1-ZIKV) encoding non-structural protein 1 (NS1) genetically fused Herpes Simplex Virus (HSV) glycoprotein D (gD) protein....
Dengue virus represents the main arbovirus affecting humans, but there are no effective drugs or available worldwide licensed vaccine formulations capable of conferring full protection against infection. Experimental studies and results generated after release anti-DENV demonstrated that induction high-titer neutralizing antibodies does not represent sole correlate that, indeed, T cell-based immune responses plays a relevant role in establishment an protective state. In this context, study...
Abstract Severe dengue virus (DENV) infection is characterized by exacerbated inflammatory responses that lead to endothelial dysfunction and plasma leakage. We have recently demonstrated Toll-like receptor 2 (TLR2) on blood monocytes senses DENV leading activation. Here, we report non-infectious immature particles, which are released in large numbers DENV-infected cells, drive activation via the TLR2 axis. show fully particles induce a rapid, within 6 hours post-infection, response PBMCs....
Targeting dendritic cells (DCs) by means of monoclonal antibodies (mAbs) capable binding their surface receptors (DEC205 and DCIR2) has previously been shown to enhance the immunogenicity genetically fused antigens. This approach repeatedly demonstrated induced immune responses passenger antigens thus represents a promising therapeutic and/or prophylactic strategy against different infectious diseases. Additionally, under experimental conditions, chimeric αDEC205 or αDCIR2 mAbs are usually...