A5032833339- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Tryptophan and brain disorders
- Cancer Immunotherapy and Biomarkers
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Toxin Mechanisms and Immunotoxins
- Escherichia coli research studies
- Mosquito-borne diseases and control
- interferon and immune responses
- Cervical Cancer and HPV Research
- Hepatitis B Virus Studies
- Microbial Inactivation Methods
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Viral Infections and Outbreaks Research
- Immune cells in cancer
- Science and Education Research
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Ferroptosis and cancer prognosis
- Bacterial Genetics and Biotechnology
- MicroRNA in disease regulation
Universidade de São Paulo
2010-2023
Hospital Universitário da Universidade de São Paulo
2022
Microsoft (Brazil)
2010
Instituto Butantan
2010
Centro de Excelência em Bioinformática
2010
Institute of Biomedical Science
2010
Biotechnology Institute
2010
As mRNA vaccines have proved to be very successful in battling the coronavirus disease 2019 (COVID-19) pandemic, this new modality has attracted widespread interest for development of potent against other infectious diseases and cancer. Cervical cancer caused by persistent human papillomavirus (HPV) infection is a major cause cancer-related deaths women, safe effective therapeutic strategies urgently needed. In present study, we compared performance three different vaccine modalities target...
Abstract Glioblastoma patients have a poor prognosis mainly due to temozolomide (TMZ) resistance. NRF2 is an important transcript factor involved in chemotherapy resistance its protective role the transcription of genes cellular detoxification and prevention cell death processes, such as ferroptosis. However, relation between iron-dependent glioma still poorly understood. Therefore, this study, we analyzed ferroptosis modulation glioblastoma cells. Two human lines (U251MG T98G) were examined...
Malignant brain tumors are among the most aggressive cancers with poor prognosis and no effective treatment. Recently, we reported oncolytic potential of Zika virus infecting destroying human central nervous system (CNS) in vitro immunodeficient mice model. However, translating this approach to humans requires pre-clinical trials another immunocompetent animal Here, analyzed safety Brazilian (ZIKVBR) intrathecal injections three dogs bearing spontaneous CNS aiming an anti-tumoral therapy. We...
The dengue virus NS1 protein has been shown to be a protective antigen under different experimental conditions but the recombinant produced in bacterial expression systems is usually not soluble and loses structural immunological features of native viral protein. In present study, leading purification refolding type 2 (DENV-2) expressed Escherichia coli are described. refolded was recovered as heat-stable dimers with preserved features, demonstrated by spectroscopic methods. addition,...
Immunotherapy has become an important ally in the fight against distinct types of cancer. However, metabolic plasticity tumor environment frequently influences efficacy therapeutic procedures, including those based on immunological tools. In this scenario, immunometabolic adjuvants arise as alternative toward development more efficient cancer therapies. Here we demonstrated that combination melatonin, a neuroimmunomodulator molecule, and indoleamine 2,3-dioxygenase (IDO) inhibitor...
Recently, Bacillus subtilis spores were shown to be endowed with strong adjuvant capacity when co-administered purified antigenic proteins. In the present study we assessed whether possess properties combined DNA vaccines. We showed that B. promoted activation of dendritic cells in vitro and induced migration pro-inflammatory after parenteral administration mice. Likewise, co-administration a vaccine encoding human papillomavirus type 16 (HPV-16) E7 protein enhanced antigen-specific CD8(+) T...
The generation of successful anticancer vaccines relies on the ability to induce efficient and long-lasting immune responses tumor antigens.In this scenario, dendritic cells (DCs) are essential cellular components in antitumor responses.Thus, delivery antigens specific DC populations represents a promising approach enhance efficiency immunotherapies.In present study, we employed antibody-antigen conjugates targeting C-type lectin receptor.For that purpose, genetically fused anti-DEC205...
Cervical cancer is a major public health problem and one of the leading causes deaths in women. Virtually all cases cervical cancer, as well growing share anal head/neck tumors, are associated with human papillomavirus (HPV) infection. Despite effectiveness, available prophylactic vaccines do not benefit women lesions or cancer. Therefore, search new immunotherapeutic approaches to treat HPV-induced tumors still priority. The present study characterizes therapeutic antitumor vaccine based on...
Type 1 herpes virus (HSV-1) glycoprotein D (gD) enhances antigen-specific immune responses, particularly CD8(+) T cell in mice immunized with DNA vaccines encoding hybrid proteins genetically fused the target antigen at a site near C-terminal end. These effects are attributed to interaction of gD entry mediator (HVEM) and concomitant blockade coinhibitory mechanism mediated by B- T-lymphocyte attenuator (BTLA). However, questions concerning requirement for endogenous synthesis or...
Shiga toxin (Stx) is considered the main virulence factor in toxin-producing Escherichia coli (STEC) infections. Previously we reported expression of biologically active Stx by eukaryotic cells vitro and vivo following transfection with plasmids encoding under control native bacterial promoter. Since stx genes are present genome lysogenic bacteriophages, here evaluated relevance bacteriophages during STEC infection. We used non-pathogenic E. K12 strain carrying a 933W mutant bacteriophage...
Although active immunotherapies are effective strategies to induce activation of CD8+ T cells, advanced stage tumors require further improvements for efficient control. Concerning the burden cancer-related Human papillomavirus (HPV), particularly high incidence and mortality cervical cancer, our group developed an approach based on a DNA vaccine targeting HPV-16 E7 oncoprotein (pgDE7h). This immunotherapy is capable inducing antitumour cell response but show only partial control in more...
The active immunotherapy concept relies on the use of vaccines that are capable inducing antitumor immunity, reversion suppressive immunological environment, and long-term memory responses. Previously, based a recombinant plasmid (pgDE7h) or purified protein (gDE7) led to regression early-established human papillomavirus (HPV)-associated tumors in preclinical model. In this work, anticancer were combined with cisplatin treat HPV-induced at advanced growth stages. effects evaluated terms...
High-risk Human papillomavirus (HPV) infections represent an important public health issue. Nearly all cervical malignancies are associated with HPV, and a range of other female male cancers, such as anogenital oropharyngeal. Aiming to treat HPV-related tumors, our group developed vaccines based on the genetic fusion HSV-1 glycoprotein D (gD) HPV-16 E7 oncoprotein (gDE7 vaccines). Despite promising antitumor results reached by gDE7 in mice, combined therapies may increase therapeutic effects...
Shiga toxin (Stx) is considered the main virulence factor in toxin-producing Escherichia coli (STEC) infections. Previously we reported expression of biologically active Stx by eukaryotic cells vitro and vivo following transfection with plasmids encoding under control native bacterial promoter. Since stx genes are present genome lysogenic bacteriophages, here evaluated relevance bacteriophages during STEC infection. We used non-pathogenic E. K12 strain carrying a 933W mutant bacteriophage...
<p>Figure S1 and S2 Legends</p>
<p>Figure S1. Immunization with gDE7 admixed poly (I:C) induces full therapeutic antitumor protection in WT, TLR4 KO and CD4+ T cells mice.</p>
<p>Figure S2. Polymixin B inhibits LPS-induced up-regulation of co-stimulatory molecules and secretion inflammatory cytokines in both mouse human DCs.</p>
<p>Figure S1. Immunization with gDE7 admixed poly (I:C) induces full therapeutic antitumor protection in WT, TLR4 KO and CD4+ T cells mice.</p>
<p>Figure S1 and S2 Legends</p>
<p>Figure S2. Polymixin B inhibits LPS-induced up-regulation of co-stimulatory molecules and secretion inflammatory cytokines in both mouse human DCs.</p>
<div>Abstract<p>Cervical cancer is a major public health problem and one of the leading causes deaths in women. Virtually all cases cervical cancer, as well growing share anal head/neck tumors, are associated with human papillomavirus (HPV) infection. Despite effectiveness, available prophylactic vaccines do not benefit women lesions or cancer. Therefore, search new immunotherapeutic approaches to treat HPV-induced tumors still priority. The present study characterizes...
<div>Abstract<p>Cervical cancer is a major public health problem and one of the leading causes deaths in women. Virtually all cases cervical cancer, as well growing share anal head/neck tumors, are associated with human papillomavirus (HPV) infection. Despite effectiveness, available prophylactic vaccines do not benefit women lesions or cancer. Therefore, search new immunotherapeutic approaches to treat HPV-induced tumors still priority. The present study characterizes...