A5055191595- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS Research and Interventions
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
- Natural product bioactivities and synthesis
- Malaria Research and Control
- Sesquiterpenes and Asteraceae Studies
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Viral Infections and Vectors
- Child Nutrition and Water Access
- Advanced Biosensing Techniques and Applications
- Plant Pathogens and Resistance
- Bacterial Infections and Vaccines
- vaccines and immunoinformatics approaches
- Yeasts and Rust Fungi Studies
Universidade de São Paulo
2004-2024
National Cancer Institute
2011-2019
National Institutes of Health
2011-2019
Fundação Oswaldo Cruz
2019
Center for Cancer Research
2011-2013
Universidade Brasil
2012
MRC Laboratory of Molecular Biology
2007
Universidade de Ribeirão Preto
2004
Because the viral DNA burden correlates with disease development, we investigated contribution of monocyte subsets (classical, intermediate, and nonclassical monocytes) to total in 22 human T cell leukemia virus type 1 (HTLV-1)-infected individuals by assessing their infectivity status, frequency, as well chemotactic phagocytic functions. All three sorted from HTLV-1-infected were positive for DNA, frequency classical monocytes was lower blood than that uninfected individuals, while...
Malignant brain tumors are among the most aggressive cancers with poor prognosis and no effective treatment. Recently, we reported oncolytic potential of Zika virus infecting destroying human central nervous system (CNS) in vitro immunodeficient mice model. However, translating this approach to humans requires pre-clinical trials another immunocompetent animal Here, analyzed safety Brazilian (ZIKVBR) intrathecal injections three dogs bearing spontaneous CNS aiming an anti-tumoral therapy. We...
Abstract The raising of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) variants led to the use COVID‐19 bivalent vaccines, which include antigens wild‐type (WT) virus, and Omicron strain. In this study, we aimed evaluate impact vaccination on neutralizing antibody (NAb) response. We enrolled 93 volunteers who had received three or four doses monovalent vaccines based original virus ( n = 61), a booster shot with vaccine 32). Serum samples collected from were subjected...
HTLV-1 orf-I is linked to immune evasion, viral replication and persistence. Examining the sequence of 160 HTLV-1-infected individuals; we found polymorphism that alters relative amounts p12 its cleavage product p8. Three groups were identified on basis p8 expression: predominantly p12, balanced expression We a significant association between with high DNA loads, correlate disease development. To determine individual roles in persistence, constructed infectious molecular clones expressing...
Human T cell leukemia virus type 1 (HTLV-1) is the ethological agent of adult leukemia/lymphoma (ATLL) and a number lymphocyte-mediated inflammatory conditions, including HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1 orf-I encodes two proteins, p8 p12, whose functions in humans are to counteract innate adaptive responses support viral transmission. However, vivo requirements for expression vary different animal models. In macaques, ablation by mutation its ATG initiation...
The main coronavirus disease 2019 (COVID-19) vaccine formulations used today are mainly based on the wild-type severe acute respiratory syndrome 2 (SARS-CoV-2) spike glycoprotein as an antigen. However, new virus variants capable of escaping neutralization activity serum antibodies elicited in vaccinated individuals have emerged. Omicron (B.1.1.529) variant caused epidemics regions world which most population has been vaccinated. In this study, we aimed to understand what determines...
This work reports for the first time production a furanoheliangolide (goyazensolide) by plant cell culture. Monitoring of goyazensolide metabolism revealed that maximum occurred during lag phase Lychnophora ericoides callus The antiproliferative activity obtained was evaluated against seven cancer lines using MTT assay. results potent cytotoxic furaheliangolide with IC50 values in range 0.06 μg/ml CEM leukemia cells to 0.75 B16 melanome cells.
Few studies have reported the molecular epidemiological characterization of HIV-1 in Northern region Brazil. The present study reports and 31 isolates from blood donors State Amazonas who donated between April 2006 March 2007. Serum/plasma samples all were screened for HIV antibodies by ELISA results confirmed Western blot analysis. Genomic DNA was extracted buffy coat using Super Quik-Gene-DNA Isolation kit. Nested PCR performed on env, gag, pol regions Gene Amp System 9700. Sequencing...
Multilayered polymeric particles work as carriers of compounds with controlled release triggered by the layer's disassembly, allowing chemical stability along transport through bloodstream and tissues. Here, we present versatile multilayered a vaccine approach against COVID-19 based on coupling recombinant form receptor binding domain (RBD) antigen SARS-CoV-2 Spike proteins. The particle core comprises poly(d,l-lactide) coated Triton X-100 polyethylenimine, prepared nanoemulsion/solvent...
The unprecedented global impact caused by SARS-CoV-2 imposed huge health and economic challenges, highlighting the urgent need for safe effective vaccines. receptor-binding domain (RBD) of is major target neutralizing antibodies vaccine formulations. Nonetheless, low immunogenicity RBD requires use alternative strategies to enhance its immunological properties. Here, we evaluated a subunit antigen generated after genetic fusing with mouse IgG antibody. Subcutaneous administration RBD-IgG led...
Abstract Background Human T-cell Leukemia Virus type 1 (HTLV-1) is the etiological agent of tropical spastic paraparesis/HTLV-associated myelopathy (HAM/TSP) that can be identified in around 0.25%–3.8% infected population. Disease progression monitored by proviral load and may depend on genetic factors, however, it not well understood why some HTLV-1 people develop disease while others do not. The present study attempts to assess molecular diversity gp46 glycoprotein HAM/TSP patients Health...
The orf-I gene of human T-cell leukemia type 1 (HTLV-1) encodes p8 and p12 has a conserved cysteine at position 39. form disulfide-linked dimers, only the monomeric forms are palmitoylated. Mutation 39 to alanine (C39A) abrogated dimerization palmitoylation both proteins. However, ability localize cell surface increase adhesion viral transmission was not affected by C39A mutation.
The C-terminal portion of the E protein, known as stem, is conserved among flaviviruses and an important target to peptide-based antiviral strategies. Since dengue (DENV) Zika (ZIKV) viruses share sequences in stem region, this study we evaluated cross-inhibition ZIKV by stem-based DV2 peptide (419–447), which was previously described inhibit all DENV serotypes. Thus, anti-ZIKV effects induced treatments with were tested both vitro vivo conditions. Molecular modeling approaches have...
Abstract Amongst the potential contribution of protein or peptide-display systems to study epitopes with relevant immunological features, RAD display system stands out as a highly stable scaffold that allows presentation constrained target peptides. Here, we employed present peptides derived from SARS-CoV-2 Spike (S) tool detect specific serum antibodies and generate polyclonal capable inhibiting infectivity in vitro. 44 linear S-derived were genetically fused (RAD-SCoV-epitopes) screened...
OPINION article Front. Public Health, 01 July 2021Sec. Health Policy https://doi.org/10.3389/fpubh.2021.693743
Dengue is an infectious disease of global health concern that continues to require surveillance. Serological testing has been used investigate dengue-infected patients, but specificity affected by the co-circulation ZIKA virus (ZIKV), which shares extensive antigen similarities. The goal this study was development a specific dengue (DENV) IgG ELISA based on multi-epitope NS1-based for antibody detection. protein (T-ΔNS1), derived from fragment NS1-protein four DENV serotypes, expressed in...
Abstract For see ChemInform in Full Text.
The human T cell lymphotropic virus type 1 (HTLV-1) infects 5 to 10 million individuals and remains without specific treatment. This retrovirus genome is composed of the genes gag, pol, env, a region known as pX. contains four open reading frames (ORFs) that encode proteins. ORF-I produces protein p12 its cleavage product, p8. In this study, we analyzed genetic diversity 32 sequences from patients with different clinical profiles. Seven amino acid changes frequency over 5% were identified:...
In the present study, we evaluated immunological responses induced by dengue vaccines under experimental conditions after delivery via a transcutaneous (TC) route. Vaccines against type 2 Dengue virus particles (DENV2 New Guinea C (NGC) strain) combined with enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) were administered to BALB/c mice in three-dose immunization regimen TC As control for parenteral administration route, other mouse groups immunized same vaccine formulation...