A5039792146- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Bacterial Genetics and Biotechnology
- SARS-CoV-2 detection and testing
- Circular RNAs in diseases
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Malaria Research and Control
- COVID-19 diagnosis using AI
- Antibiotic Resistance in Bacteria
- Virus-based gene therapy research
- Amphibian and Reptile Biology
- Cancer-related molecular mechanisms research
- Biochemical and Structural Characterization
- Business and Management Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Genetic diversity and population structure
- MicroRNA in disease regulation
- Infant Nutrition and Health
- Probiotics and Fermented Foods
Universidade Estadual de Santa Cruz
2015-2024
Universidade Federal da Paraíba
2022
Universidade Federal de Minas Gerais
2021
Universidade de São Paulo
2008-2020
Centro de Excelência em Bioinformática
2010
Institute of Biomedical Science
2004
Abstract Early diagnosis of COVID-19 in suspected patients is essential for contagion control and damage reduction strategies. We investigated the applicability attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy associated with machine learning oropharyngeal swab suspension fluid to predict positive samples. The study included samples 243 from two Brazilian States. Samples were transported by using different viral transport mediums (liquid 1 or 2). Clinical was...
Sepsis continues to be a significant public health challenge despite advances in understanding its pathophysiology and management strategies. Therefore, this study evaluated the value of cell-free nuclear DNA (cf-nDNA) mitochondrial (cf-mtDNA) for assessing severity prognosis sepsis. Ninety-four patients were divided into three groups: infection (n = 32), sepsis 30), septic shock 32). Plasma samples collected at time diagnosis, cfDNA concentrations determined by qPCR assay. The results...
Bacillus subtilis spores have received growing attention regarding potential biotechnological applications, including the use as probiotics and in vaccine formulations. B. also been shown to behave particulate adjuvants, promoting increase of antibody responses after co-administration with antigens either admixed or adsorbed on spore surface. In this study, we further evaluated immune modulatory properties using a recombinant HIV gag p24 protein model antigen. The adjuvant effects were not...
Abstract The raising of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) variants led to the use COVID‐19 bivalent vaccines, which include antigens wild‐type (WT) virus, and Omicron strain. In this study, we aimed evaluate impact vaccination on neutralizing antibody (NAb) response. We enrolled 93 volunteers who had received three or four doses monovalent vaccines based original virus ( n = 61), a booster shot with vaccine 32). Serum samples collected from were subjected...
Bacillus subtilis spores have been used as safe and heat-resistant antigen delivery vectors. Nonetheless, the oral administration of typically induces weak immune responses to passenger antigens, which may be attributed fast transit through gastrointestinal tract. To overcome this limitation, we developed B. capable binding gut epithelium by means expressing bacterial adhesins on spore surface. The resulting bound in vitro intestinal cells, showed a longer mouse tract, interacted with...
ABSTRACT Globally, enterotoxigenic Escherichia coli (ETEC) is a leading cause of childhood and travelers' diarrhea, for which an effective vaccine needed. Prevalent intestinal colonization factors (CFs) such as CFA/I fimbriae heat-labile enterotoxin (LT) are important virulence protective antigens. We tested the hypothesis that donor strand-complemented CfaE (dscCfaE), stabilized form fimbrial tip adhesin, antigen, using lethal neonatal mouse ETEC challenge model passive dam vaccination. For...
Introduction: Breast cancer represents the most prevalent malignancy among women. Recent advancements in translational research have focused on identification of novel biomarkers capable providing valuable insights into patient outcomes. Furthermore, comprehensive investigations aimed at discovering miRNAs, unraveling their biological functions, and deciphering target genes significantly contributed to our understanding roles miRNAs play tumorigenesis. Consequently, these facilitated way for...
The Flavivirus genus is composed by viral serocomplexes with relevant global epidemiological impact. Many areas of the world present both, vector fauna and geographical conditions compatible co-circulation, importing, emergence epidemics flaviviruses different serocomplexes. In this study, we aimed to identify immunological determinants patterns immune response possibly involved in flavivirus serocomplex cross-protection. We searched B T cells epitopes which were thoroughly shown be control....
A third vaccine dose against COVID-19 is already a reality in some countries around the world. In this study, we aimed to evaluate effectiveness of Brazilian immunization policy for COVID-19, which involves booster shot. Participants (n = 210) provided serum samples, were subjected enzyme-linked immunosorbent assay (ELISA). Immunological profiles defined as individuals with or without previous SARS-CoV-2 infection who received at least one regimens AstraZeneca, CoronaVac, CoronaVac plus shot...
Abstract Background Phylogenetic studies indicate bats as original hosts of SARS-CoV-2. However, it remains unclear whether other animals, including pets, are crucial in the spread and maintenance COVID-19 worldwide. Methods In this study, we analyzed first fatal case a SARS-CoV-2 FeLV co-infection an eight-year-old male cat. We carried out clinical evaluation several laboratory analyses. Results As main results, observed animal presenting severe acute respiratory syndrome lesions organs,...
Colonization surface antigens (CSs) represent key virulence-associated factors of enterotoxigenic Escherichia coli (ETEC) strains. They are required for gut colonization, the first step diarrhoeal disease process induced by these bacteria. One most prevalent CSs is CS21, or longus, a type IV pili associated with bacterial self-aggregation, protection against environmental stresses, biofilm formation and adherence to epithelial cell lines. The objectives this study were assess role CS21 in...
This research addresses two questions: (1) how El Niño Southern Oscillation (ENSO) affects climate variability and it influences dengue transmission in the Metropolitan Region of Recife (MRR), (2) whether epidemic MRR municipalities has any connection synchronicity.Wavelet analysis cross-correlation were applied to characterize seasonality, multiyear cycles, relative delays between series. study was developed into distinct periods. Initially, we performed periodic incidence intercity...
The expression of flagella correlates with different aspects bacterial pathogenicity, ranging from adherence to host cells activation inflammatory responses by the innate immune system. In present study, we investigated role in an atypical enteropathogenic Escherichia coli (aEPEC) strain (serotype O51:H40) human enterocytes. Accordingly, isogenic mutants deficient flagellin (FliC), flagellar structural subunit; cap protein (FliD); or MotAB proteins, involved control motion, were generated...
The main coronavirus disease 2019 (COVID-19) vaccine formulations used today are mainly based on the wild-type severe acute respiratory syndrome 2 (SARS-CoV-2) spike glycoprotein as an antigen. However, new virus variants capable of escaping neutralization activity serum antibodies elicited in vaccinated individuals have emerged. Omicron (B.1.1.529) variant caused epidemics regions world which most population has been vaccinated. In this study, we aimed to understand what determines...
Background With the rapid adoption of electronic medical records (EMRs), there is an ever-increasing opportunity to collect data and extract knowledge from EMRs support patient-centered stroke management. Objective This study aims compare effectiveness state-of-the-art automatic text classification methods in classifying prediction clinical patient outcomes extraction characteristics EMRs. Methods Our addressed computational problems information classification. We identified essential tasks...
Shiga toxin (Stx) is considered the main virulence factor in toxin-producing Escherichia coli (STEC) infections. Previously we reported expression of biologically active Stx by eukaryotic cells vitro and vivo following transfection with plasmids encoding under control native bacterial promoter. Since stx genes are present genome lysogenic bacteriophages, here evaluated relevance bacteriophages during STEC infection. We used non-pathogenic E. K12 strain carrying a 933W mutant bacteriophage...
The four serotypes of Dengue virus (DENV1-4) are arboviruses (arthropod-borne viruses) that belong to the Flavivirus genus, Flaviviridae family. They causative agents an infectious disease called dengue, important global public health problem with significant social-economic impact. Thus, development safe and effective dengue vaccines is a priority according World Health Organization. Only one anti-dengue vaccine has already been licensed in endemic countries two formulations under phase III...