A5059092884- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Liver physiology and pathology
- Virology and Viral Diseases
- RNA modifications and cancer
- Viral Infections and Vectors
- Tissue Engineering and Regenerative Medicine
- Neurogenetic and Muscular Disorders Research
- Exercise and Physiological Responses
- Neonatal Respiratory Health Research
- Genetics and Neurodevelopmental Disorders
- MicroRNA in disease regulation
- RNA Research and Splicing
- Congenital heart defects research
- Muscle Physiology and Disorders
- Electrospun Nanofibers in Biomedical Applications
- Pelvic floor disorders treatments
- Phytochemistry Medicinal Plant Applications
- Cancer-related gene regulation
- Dental Trauma and Treatments
- Down syndrome and intellectual disability research
Universidade de São Paulo
2015-2024
Instituto Biológico
2019-2020
Universidade Federal de Minas Gerais
2000-2015
Abstract Congenital Zika syndrome (CZS) causes early brain development impairment by affecting neural progenitor cells (NPCs). Here, we analyze NPCs from three pairs of dizygotic twins discordant for CZS. We compare RNA-Seq the derived CZS-affected and CZS-unaffected twins. Prior to virus (ZIKV) infection CZS babies show a significantly different gene expression signature mTOR Wnt pathway regulators, key neurodevelopmental program. Following ZIKV in vitro infection, affected individuals have...
The liver is responsible for many metabolic, endocrine and exocrine functions. Approximately 2 million deaths per year are associated with failure. Modern 3D bioprinting technologies allied autologous induced pluripotent stem cells (iPS)-derived grafts could represent a relevant tissue engineering approach to treat end stage disease patients. However, protocols that accurately recapitulates liver's epithelial parenchyma through still underdeveloped. Here we evaluated the impacts of using...
Zika virus (ZIKV) is largely known for causing brain abnormalities due to its ability infect neural progenitor stem cells during early development. Here, we show that ZIKV also capable of infecting and destroying stem-like cancer from aggressive human embryonal tumors the central nervous system (CNS). When evaluating oncolytic properties Brazilian strain (ZIKVBR) against breast, prostate, colorectal, CNS tumor cell lines, verified a selective infection followed by massive death. ZIKVBR was...
Liver organoid technology holds great promises to be used in large-scale population-based drug screening and future regenerative medicine strategies. Recently, some studies reported robust protocols for generating isogenic liver organoids using parenchymal non-parenchymal cells derived from induced pluripotent stem (iPS) or adult primary cells. However, the use of whole iPS-derived could represent challenges a translational perspective. Here, we evaluated influence versus heterogenic cells,...
Malignant brain tumors are among the most aggressive cancers with poor prognosis and no effective treatment. Recently, we reported oncolytic potential of Zika virus infecting destroying human central nervous system (CNS) in vitro immunodeficient mice model. However, translating this approach to humans requires pre-clinical trials another immunocompetent animal Here, analyzed safety Brazilian (ZIKVBR) intrathecal injections three dogs bearing spontaneous CNS aiming an anti-tumoral therapy. We...
Biallelic loss-of-function mutations in the RNA-binding protein EIF4A3 cause Richieri-Costa-Pereira syndrome (RCPS), an autosomal recessive condition mainly characterized by craniofacial and limb malformations. However, pathogenic cellular mechanisms responsible for this are entirely unknown. Here, we used two complementary approaches, patient-derived induced pluripotent stem cells (iPSCs) conditional Eif4a3 mouse models, to demonstrate that defective neural crest cell (NCC) development...
OBJETIVOS: A proposta deste trabalho é investigar o perfil socioeconômico, motivo de estudar medicina, a opção por especialidade e residência médica preferência em trabalhar como profissional liberal ou assalariado entre os estudantes medicina da Universidade Federal Minas Gerais (UFMG). MÉTODO: Durante ano 1997, realizou-se estudo comparativo UFMG do 5º período, iniciando ciclo clínico, aqueles internato, terminando clínico. Como instrumento foi utilizado um questionário distribuído todos...
Background Experimental studies on humans have shown that recurrent pain is associated with altered perception. We measured the pressure threshold in regions of body surface a group children who had abdominal and chronic or disease but no pain. Methods Each consisted 45 boys 55 girls ranging age from 5 to 15.8 years. The were trapezius, deltoid, supraspinous muscles, nine areas wall, median part tibias. Using an algometer, was applied through rubber disc area 1 cm2 at rate 0.5 kg/cm2/s....
To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance karyotypic abnormalities or loss pluripotency.Cells were kept in culture for over 50 passages, following structured chronogram passage and monitoring cell growth by population doubling time calculation confluence. Standard procedures as embryonic body formation, karyotyping pluripotency markers expression evaluated order...
Zika virus (ZIKV) causes congenital syndrome (CZS), which is characterized by fetal demise, microcephaly and other abnormalities. ZIKV in the pregnant woman circulation must cross placental barrier that includes endothelial cells trophoblasts, order to reach fetus. CZS occurs ~1-40% of cases women infected ZIKV, suggesting mothers' infection during pregnancy not deterministic for phenotype Therefore, susceptibility factors might be involved, including host genetic background. We have...
Hepatoblastoma is an embryonal liver tumor carrying few genetic alterations. We previously disclosed in hepatoblastomas a genome-wide methylation dysfunction, characterized by hypermethylation at specific CpG islands, addition to low-level hypomethylation pattern non-repetitive intergenic sequences, comparison non-tumoral tissues, shedding light into crucial role for epigenetic dysregulation this type of cancer. To explore the underlying mechanisms possibly related aberrant modifications, we...
Neural crest cells (NCCs) contribute to several tissues during embryonic development. NCC formation depends on activation of tightly regulated molecular programs at the neural plate border (NPB) region, which initiate specification and epithelial-to-mesenchymal transition (EMT). Although approaches investigate NCCs have been devised, these early events remain largely unknown in humans, currently available cellular models not investigated EMT. Here, we report that E6 induction protocol...
Hepatoblastomas exhibit the lowest mutational burden among pediatric tumors. We previously showed that epigenetic disruption is crucial for hepatoblastoma carcinogenesis. Our data revealed hypermethylation of nicotinamide N-methyltransferase, a highly expressed gene in adipocytes and hepatocytes. The expression pattern role N-methyltransferase liver tumors have not yet been explored, this study aimed to evaluate effect hepatoblastomas. evaluated 45 hepatoblastomas 26 non-tumoral samples....
Article Screening for Congenital Hypothyroidism in Extreme Premature and/or Very Low Birth Weight Newborns: The Importance of a Specific Protocol was published on February 1, 2010 the journal Journal Pediatric Endocrinology and Metabolism (volume 23, issue 1-2).
The objective of the present study was to evaluate associations between fiber intake, colonic transit time and stool frequency. Thirty-eight patients aged 4 14 years were submitted alimentary evaluation measurement time. median intake total sample age + 10.3 g/day. Only 18.4% subjects presented a daily dietary below levels recommended by American Health Foundation. In this group, left shorter than in group with higher (11 vs 17 h, P = 0.067). correlation frequency negative weak for colon (r...
Asthma morbidity is high, partly due to low adherence inhaled corticosteroids (ICS). This study aims assess rates and factors related ICS over time in asthmatic children adolescents. Methods: A concurrent cohort was carried out for 24 months 168 randomly selected patients suffering from persistent moderate asthma. All of them were given beclomethasone dipropionate (BDP) free charge. Adherence verified by pharmacy records (doses filled/doses prescribed). multivariate analysis evaluated with...
To investigate whether single nucleotide polymorphisms (SNPs) within the interleukin-1 gene cluster (IL1) are associated with occurrence and severity of inflammatory external root resorption (IERR) after replantation avulsed permanent teeth.Indexes IERR were radiographically assessed in 182 mature replanted teeth from 146 patients at onset endodontic therapy. DNA was extracted buccal mucosa cells genotyped using TaqMan probes-based assays for SNPs IL1A -889C/T (rs 180058), IL1B +3954C/T...
Amyotrophic lateral sclerosis type 8 (ALS8) is an autosomal dominant form of ALS, which caused by pathogenic variants in the VAPB gene. Here we investigated five ALS8 patients, classified as 'severe' and 'mild' from a gigantic Brazilian kindred, carrying same mutation but displaying different clinical courses. Copy number variation whole exome sequencing analyses such individuals ruled out previously described genetic modifiers pathogenicity. After deriving induced pluripotent stem cells...