A5055381647- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Escherichia coli research studies
- Herpesvirus Infections and Treatments
- Tryptophan and brain disorders
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Cancer Immunotherapy and Biomarkers
- Viral gastroenteritis research and epidemiology
- Innovative Teaching Methods
- Enterobacteriaceae and Cronobacter Research
- Mesenchymal stem cell research
- Cancer, Stress, Anesthesia, and Immune Response
- Salmonella and Campylobacter epidemiology
- Osteoarthritis Treatment and Mechanisms
- Gut microbiota and health
- Genetics, Bioinformatics, and Biomedical Research
- Sepsis Diagnosis and Treatment
- interferon and immune responses
- Periodontal Regeneration and Treatments
- Cervical Cancer and HPV Research
- Antibiotic Resistance in Bacteria
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Radio, Podcasts, and Digital Media
Universidade de São Paulo
2014-2024
Instituto Butantan
2015-2024
Vall d'Hebron Institute of Oncology
2024
University of Cambridge
2023
Hospitais da Universidade de Coimbra
2020
Hospital Universitario La Paz
2003-2019
Universidad Autónoma de Madrid
2019
Trinity College Dublin
2018
Research Institute Hospital 12 de Octubre
2000-2018
Krajská Zdravotní
2018
As mRNA vaccines have proved to be very successful in battling the coronavirus disease 2019 (COVID-19) pandemic, this new modality has attracted widespread interest for development of potent against other infectious diseases and cancer. Cervical cancer caused by persistent human papillomavirus (HPV) infection is a major cause cancer-related deaths women, safe effective therapeutic strategies urgently needed. In present study, we compared performance three different vaccine modalities target...
Malignant brain tumors are among the most aggressive cancers with poor prognosis and no effective treatment. Recently, we reported oncolytic potential of Zika virus infecting destroying human central nervous system (CNS) in vitro immunodeficient mice model. However, translating this approach to humans requires pre-clinical trials another immunocompetent animal Here, analyzed safety Brazilian (ZIKVBR) intrathecal injections three dogs bearing spontaneous CNS aiming an anti-tumoral therapy. We...
Abstract Here, we present a case study of undergraduate biomedical students engaging in the Adopt Bacterium active methodology. The was designed and authored by students, associating heroes antiheroes with Escherichia coli to highlight its dual role human health. By integrating elements geek culture, developed multimedia educational content, including social media posts, interactive chalkboard presentation, gamified seminar, comic book. This creative approach enabled deeper understanding...
Immunotherapy has become an important ally in the fight against distinct types of cancer. However, metabolic plasticity tumor environment frequently influences efficacy therapeutic procedures, including those based on immunological tools. In this scenario, immunometabolic adjuvants arise as alternative toward development more efficient cancer therapies. Here we demonstrated that combination melatonin, a neuroimmunomodulator molecule, and indoleamine 2,3-dioxygenase (IDO) inhibitor...
Bacterial translocation has been shown to occur in critically ill patients after extensive trauma, shock, sepsis, or thermal injury. The present study investigates mesenteric microcirculatory dysfunctions, the bacterial phenomenon, and hemodynamic/metabolic disturbances a rat model of intestinal obstruction ischemia. Anesthetized (pentobarbital 50 mg/kg, i.p.) male Wistar rats (250–350 g) were submitted laparotomy without (Sham) evaluated 24 hours later. was assessed by culture lymph nodes...
Indoleamine 2,3‐dioxygenase 1 ( IDO 1), the rate‐limiting enzyme of tryptophan catabolism, has been strongly associated with progression malignancy and poor survival in melanoma patients. As a result, is leading target for interventions aimed at restoring immune surveillance. Here, scenario involving we report that melatonin biosynthesis driven by 1‐methyl‐tryptophan (1‐ MT ), competitive inhibitor 1, human fibroblasts, melanocytes cells. In addition to biosynthesis, 1‐ induced expression...
Down syndrome (DS) is the most common syndromic immunodeficiency with an increased risk of infection, mortality from sepsis, and autoinflammation. Innate immune function altered in DS therefore we examined responses CD11b Toll like receptor 4 (TLR-4), which are important cell surface markers upregulated response to Lipopolysaccharide (LPS) endotoxin, immunomodulator melatonin. Neutrophil monocyte LPS melatonin children who were clinically stable compared age-matched controls. Whole blood was...
Important features of the enteroinvasive Escherichia coli (EIEC) phenotype and gene expression likely to confer EIEC with a lower ability cause disease than Shigella flexneri were described here for first time. To confirm pathogenicity EIEC, we have analyzed keratoconjunctivitis developed in guinea-pigs or S. flexneri. induced more pronounced proinflammatory response, whereas mild form disease. showed significantly less efficient cell-to-cell Caco-2 dissemination when compared Plaques formed...
Recently, Bacillus subtilis spores were shown to be endowed with strong adjuvant capacity when co-administered purified antigenic proteins. In the present study we assessed whether possess properties combined DNA vaccines. We showed that B. promoted activation of dendritic cells in vitro and induced migration pro-inflammatory after parenteral administration mice. Likewise, co-administration a vaccine encoding human papillomavirus type 16 (HPV-16) E7 protein enhanced antigen-specific CD8(+) T...
The "Adopt a Bacterium" project is based on the use of social network as tool in Microbiology undergraduate education, improving student learning and encouraging students to participate collaborative learning. approach involves active participation both teachers, emphasizing knowledge exchange, widely used media. Students were organized groups asked adopt specific bacterial genus and, subsequently, submit posts about "adopted genus". formative assessment posting information Facebook®,...
The generation of successful anticancer vaccines relies on the ability to induce efficient and long-lasting immune responses tumor antigens.In this scenario, dendritic cells (DCs) are essential cellular components in antitumor responses.Thus, delivery antigens specific DC populations represents a promising approach enhance efficiency immunotherapies.In present study, we employed antibody-antigen conjugates targeting C-type lectin receptor.For that purpose, genetically fused anti-DEC205...
Cervical cancer is a major public health problem and one of the leading causes deaths in women. Virtually all cases cervical cancer, as well growing share anal head/neck tumors, are associated with human papillomavirus (HPV) infection. Despite effectiveness, available prophylactic vaccines do not benefit women lesions or cancer. Therefore, search new immunotherapeutic approaches to treat HPV-induced tumors still priority. The present study characterizes therapeutic antitumor vaccine based on...
Tryptophan (TRP) is essential for many physiological processes, and its metabolism changes in some diseases such as infection cancer. The most studied aspects of TRP are the kynurenine serotonin pathways. A minor metabolic route, tryptamine N,N-dimethyltryptamine (DMT) biosynthesis, has received far less attention, probably because very low amounts these compounds detected only tissues, which led them to be collectively considered trace amines. In a previous study, we showed...
In this report, we analyzed the prevalence of sat gene in 336 Escherichia coli samples collected from stools children with and without diarrhea Brazil 100 uropathogenic E. strains. The results show a high correlation between diffusely adhering (DAEC) presence (44%) intestinal isolates. DAEC strain FBC114 expresses 107-kDa protein, which showed 98% homology Sat.
In this study, we performed fliC PCR-restriction fragment length polymorphism (RFLP) to investigate whether technique would be better than classic serotyping for the characterization of H antigen in enterotoxigenic Escherichia coli (ETEC) strains. We showed that genes from ETEC strains can characterized by restriction analysis their polymorphism. Only one allele gene was found each flagellar antigen, with exception H21. Nonmotile could also using molecular technique. Moreover, determination...
Cyanide is a toxic compound that converted to the non-toxic thiocyanate by rhodanese enzyme. Rhodaneses belong family of transferases (sulfurtransferases), which are largely studied. The sulfur donor defines subfamily these enzymes as thiosulfate:cyanide sulfurtransferases or rhodaneses (TSTs) 3-mercaptopyruvate sulfurtransfeases (MSTs). In Mycobacterium tuberculosis, causative agent gene Rv0815c encodes protein CysA2, putative uncharacterized sulfurtransferase belongs essential assimilation...
Among skin cancers,
 melanoma has the highest mortality rate. The heterogeneous genetic melanoma
 background leads to a tumor-propagating capacity particularly important in
 maintaining therapeutic resistance, and tumor recurrence. identification of
 efficient molecules able control progress represents an important
 opportunity for new strategies, in combination with the
 current standard-of-care treatments. In this context,
 several studies have reported...