Doxorubicin-induced cardiomyopathy (DOX-IC) is a significant and common complication in patients undergoing chemotherapy, leading to cardiac remodeling reduced heart function. We hypothesized that the intrapericardial injection of hydrogels derived from decellularized extracellular matrix (dECM) loaded with adipose tissue-derived stromal cells (ASC) their secretome dampens or reverses progression DOX-IC. DOX-IC was induced Wistar male rats through ten weekly intra-peritoneal injections...

Diabetic patients have increased susceptibility to infection, which may be related impaired inflammatory response observed in experimental models of diabetes, and restored by insulin treatment. The goal this study was investigate whether regulates transcription cytokines intercellular adhesion molecule 1 (ICAM-1) via nuclear factor-κB (NF-κB) signaling pathway Escherichia coli LPS-induced lung inflammation. male Wistar rats (alloxan, 42 mg/kg, i.v., 10 days) controls were instilled...

Bacterial translocation has been shown to occur in critically ill patients after extensive trauma, shock, sepsis, or thermal injury. The present study investigates mesenteric microcirculatory dysfunctions, the bacterial phenomenon, and hemodynamic/metabolic disturbances a rat model of intestinal obstruction ischemia. Anesthetized (pentobarbital 50 mg/kg, i.p.) male Wistar rats (250–350 g) were submitted laparotomy without (Sham) evaluated 24 hours later. was assessed by culture lymph nodes...

OBJECTIVE:Experimental findings support clinical evidence that brain death impairs the viability of organs for transplantation, triggering hemodynamic, hormonal, and inflammatory responses.However, several these events could be consequences death-associated trauma.This study investigated microcirculatory alterations systemic markers in brain-dead rats influence associated trauma.METHOD: Brain was induced using intracranial balloon inflation; sham-operated were trepanned only.After 30 or 180...

Several studies have been performed to unravel the association between diabetes and increased susceptibility infection. This study aimed investigate effect of insulin on local environment after cecal ligation puncture (CLP) in rats.Diabetic (alloxan, 42 mg/kg i.v., 10 days) non-diabetic (control) male Wistar rats were subjected a two-puncture CLP procedure 6 h later, following analyses performed: (a) total differential cell counts peritoneal lavage (PeL) bronchoalveolar (BAL) fluids; (b)...

We examined the effects of hypertonic saline (HS) on inflammatory, metabolic variables, and bacterial translocation (BT) in rats submitted to intestinal obstruction ischemia (IO). Male Wistar were IO treated, 2 h thereafter, with lactated Ringer's (LR) (4 mL/kg per 5 min, i.v.) or HS (7.5% NaCl, 4 i.v.). Twenty-four hours after IO, also enterectomy/enteroanastomosis resection necrotized small bowel. Leukocyte-endothelial interactions investigated by intravital microscopy expression...

This article aims to report the management of anesthesia in a German Spitz dog with patent ductus arteriosus, initial aim castrating animal question. The surgical procedure was performed using 3-clamp technique described literature and occurred without complications. For anesthetic protocol, pre-anesthetic medication intramuscular methadone at dose 0.2 mg/kg chosen; presented mild sedation after (PAM). induction, intravenous propofol used 4 maintenance by inhalation isoflurane, adjusting...

OBJECTIVES: Brain death is typically followed by autonomic changes that lead to hemodynamic instability, which likely associated with microcirculatory dysfunction and inflammation.We evaluated the role of microcirculation in inflammatory events occur after brain effects storm inhibition via thoracic epidural blockade on mesenteric responses.METHODS: Male Wistar rats were anesthetized mechanically ventilated.Brain was induced intracranial balloon inflation.Bupivacaine (brain death-thoracic...

After brain death (BD) donors usually experience cardiac dysfunction, which is responsible for a considerable number of unused organs. Causes this dysfunction are not fully understood. Some authors argue that autonomic storm with severe hemodynamic instability leads to inflammatory activation and myocardial dysfunction.To investigate the hypothesis thoracic epidural anesthesia blocks improves graft condition by reducing response.Twenty-eight male Wistar rats (250-350 g) allocated four groups...

Experimental findings support the evidence of a persistent leucopenia triggered by brain death (BD). This study aimed to investigate leucocyte behaviour in bone marrow and blood after BD rats. was induced using intracranial balloon catheter inflation. Sham-operated (SH) rats were trepanned only. Thereafter cells harvested every six hours from femoral cavity used for total differential counts. They analysed further flow cytometry characterize lymphocyte subsets, granulocyte adhesion molecules...

Brain death (BD) triggers important hemodynamic and inflammatory alterations, compromising the viability of organs suitable for transplantation. To better understand microcirculatory alterations in donor lungs caused by BD. The present study investigated pulmonary microcirculation a rodent model BD via intravital microscopy.Male Wistar rats were anaesthetized mechanically ventilated. They trepanned was induced through increase intracranial pressure. As control group, sham-operated (SH) only....

Epidural anesthesia is a type of locoregional anesthetic technique used to assist in trans- and postoperative analgesia, blocking surgical nociception directly the nerve roots that leave spinal cord using local anesthetics. The existing approaches this are thoracolumbar, lumbosacral sacrococcygeal, latter showing greater safety due absence medullary cone dural sac. With this, present report aimed demonstrate epidural through sacrococcygeal approach as safer option for small dogs such as,...

Feline Lower Urinary Tract Disease (FLUTD) is the main cause of urethral obstruction in this species, including idiopathic obstructions, plugs, urolithiasis and other mechanical such as strictures neoplasms. This predominance directly related to young middle-aged males, due male's urethra being longer narrower, increasing likelihood interference. Clinical signs often include hematuria, polyuria and, most importantly, stranguria, along with a full bladder. In more severe cases, cardiovascular...

Due to the frequency of male felines suffering from recurrent urethral obstructions, perineal urethrostomy surgery appears be a viable treatment for permanent unclogging urethra. The following work aims report case utilizing locoregional block pudendal nerve as means provide regional analgesia feline associated with penectomy. patient was premedicated Methadone 0.15 mg/kg and Acepromazine 0.01 mg/kg, induced propofol 3mg/kg Ketamine 1.5mg/kg, anesthesia maintained Isoflurane. No severe...

Effects of sympathectomy on myocardium remodeling and functionMaurı ´cio Rodrigues

Pulmonary arterial hypertension (PAH) is a cardiopulmonary disease that affects the pulmonary vasculature, leading to increased afterload and eventually right ventricular (RV) remodelling failure. Bilateral sympathectomy (BS) has shown promising results in dampening cardiac dysfunction several heart failure models. In present study, we investigated whether BS reduces mitigates RV failure.PAH was induced male Wistar rats by intraperitoneal injection of monocrotaline. Rats were divided into 3...

Brain death (BD) is associated with systemic inflammatory compromise, which might affect the quality of transplanted organs. This study investigated expression profile cardiac microRNAs (miRNAs) after BD, and their relationship observed decline in myocardial function changes induced by hypertonic saline solution (HSS) treatment.Wistar rats were assigned to sham-operation (SHAM) or submitted BD without administration HSS. Cardiac was assessed for 6 h left ventricular (LV) pressure-volume...

Sympathetic activity influences the post-infarction ventricular remodelling and use of beta-blockers has shown beneficial effects on left ventricle (LV) function after myocardial infarction (MI). Nevertheless, its some limitations may cause adverse in certain patients.