Abstract Bone formation is a coordinated process involving various biological factors. We have developed scaffold system capable of sustained and localized presentation osteogenic (BMP-4) angiogenic (VEGF) growth factors human bone marrow stromal cells to promote at an ectopic site. Combined delivery these significantly enhanced compared with other conditions. Introduction: Tissue regeneration entails complex interactions between multiple signals materials platforms. Orchestrating the may...

A new class of peptide gene delivery agents were developed by inserting multiple cysteine residues into short (dp 20) synthetic peptides. Substitution one to four for lysine in Cys-Trp-Lys18 resulted low molecular weight DNA condensing peptides that spontaneously oxidize after binding plasmid form interpeptide disulfide bonds. The stability cross-linked condensates increased proportion the number cysteines incorporated peptide. Disulfide bond formation led a decrease particle size relative...

Cationic peptides possessing a single cysteine, tryptophan, and lysine repeat were synthesized to define the minimal peptide length needed mediate transient gene expression in mammalian cells. The N-terminal cysteine each was either alkylated or oxidatively dimerized produce chains of 3, 6, 8, 13, 16, 18, 26, 36 residues. Each synthetic studied for its ability condense plasmid DNA compared polylysine19 cationic lipids establish relative vitro transfer efficiency HepG2 COS 7 Peptides with...

Cross-linking peptides have been developed by inserting multiple Cys residues into a 20 amino acid condensing peptide that polymerizes through disulfide bond formation when bound to DNA resulting in small, highly stable condensates mediate efficient vitro gene transfer [McKenzie et al. (2000) J. Biol. Chem. 275, 9970-9977]. In the present study, minimal of four Lys and two terminal was found substitute for Cys-Trp-(Lys)(17)-Cys, with similar particle size expression HepG2 cells. Substitution...

A new method of determining the oligosaccharide composition commercial glycosaminoglycan heparin is described in which was first depolymerized using lyase (EC 4.2.2.7), and then analysed by a single h.p.l.c. step. All 20 porcine bovine heparins examined were found to contain small number major components, on average comprised 86% their mass. The five most abundant oligosaccharides have defined chemical structures. Although relative abundance varied, surprisingly similar. Porcine, bovine,...

Liquid chromatography/mass spectrometry (LC/MS) is applied to the analysis of complex mixtures oligosaccharides obtained through controlled, heparinase-catalyzed depolymerization heparin. Reversed-phase ion-pairing chromatography, utilizing a volatile mobile phase, results in high resolution separation highly sulfated, heparin-derived oligosaccharides. Simultaneous detection by UV absorbance and electrospray ionization-mass (ESI-MS) provides important structural information on...

Heparin-derived oligosaccharides, prepared by using flavobacterial heparinase, having a high degree of heterogeneity (sequence variability) were resolved into sharp well-defined bands polyacrylamide gel electrophoresis (PAGE). The use stacking and high-density-pore-gradient resolving was primarily responsible for the success this separation. Low-Mr standards known structure polymerization (dp) 2-6 used to establish that separation on gradient PAGE dependent molecular size. High-Mr...

Three derivatives of a triantennary glycopeptide, each containing single uniquely located 6-amino-galactose residue at either position 6', 6, or 8, were modified the 6-amino group by attachment photolyzable reagent and radiolabeled iodination tyrosine. These allowed to bind asialoglycoprotein receptor isolated rat hepatocytes photolyzed for affinity labeling. (formula; see text) Each probe specifically labeled major (RHL1) minor (RHL2/3) subunits which comprise receptor. A attached galactose...

Nonviral delivery vectors are attractive for gene therapy approaches in tissue engineering, but suffer from low transfection efficiency and short-term expression. We hypothesized that the sustained of poly(ethylenimine) (PEI)-condensed DNA three-dimensional biodegradable scaffolds encourage cell infiltration could greatly enhance To test this hypothesis, a PEI-condensed plasmid encoding β-galactosidase was incorporated into porous poly(lactide-co-glycolide) (PLG) scaffolds, using gas foaming...

The structure of heparin was examined by characterizing a disaccharide and five the more than dozen tetrasaccharide components obtained its depolymerization with flavobacterial heparinase. Enzymic porcine mucosal results in mixture di-, tetra-, hexa- higher oligo-saccharides. di- tetra-saccharide represent 75mol/100mol these fragments. Ion-exchange chromatography indicates presence only one disaccharide, deltaIdu2S(1→4)-alpha-D-GlcNS6S (where Idu is iduronic acid, deltaIdu...

Abstract Polyethylenimine (PEI) was combined with plasmid DNA and freeze dried following the addition of sucrose as a lyoprotectant pore‐forming agent. Freeze‐dried PEI condensates were dry mixed granular polylactideglycolic acid (PLGA) then compression molded sponged to encapsulated DNA. A measurement elastic modulus indicated that 91 wt% substituted for 95 sodium chloride porogen, resulting in PLGA sponges mechanical 100 kPa. The retained (80%) within prepared during leaching subsequent...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTargeted Gene Delivery with a Low Molecular Weight Glycopeptide CarrierManpreet S. Wadhwa, Daren L. Knoell, Anthony P. Young, and Kevin G. RiceCite this: Bioconjugate Chem. 1995, 6, 3, 283–291Publication Date (Print):May 1, 1995Publication History Published online1 May 2002Published inissue 1 1995https://pubs.acs.org/doi/10.1021/bc00033a008https://doi.org/10.1021/bc00033a008research-articleACS PublicationsRequest reuse permissionsArticle...

The 26 amino acid hemolytic melittin peptide was converted into a gene transfer that binds to DNA and polymerized through disulfide bond formation. Melittin analogues were synthesized by the addition of one four Lys repeats at either C- or N-subterminal end along with terminal Cys residues. able bind polymerize on plasmids resulting in formation condensates. In absence DNA, retained their red blood cell potency but inactive when bound plasmid DNA. vitro efficiency mediated poly-melittin...

This paper demonstrates that heparin-oligosaccharides with low anticoagulant activity have a high capacity to inhibit activation of the amplification pathway complement in vitro. We prepared by partial depolymerization heparin using purified flavobacterial heparinase. The resulting oligosaccharide mixture was then fractionated strong anion exchange-high pressure liquid chromatography produce individual components this mixture, degree polymerization ranging from 2 16. These were examined for...

Abstract: In a previous report (M.S. Wadhwa et al . (1997) Bioconjugate Chem. 8, 81–88), we synthesized panel of polylysine‐containing peptides and determined that minimal repeating lysine chain 18 residues followed by tryptophan an alkylated cysteine residue (AlkCWK ) resulted in the formation optimal size (78 nm diameter) plasmid DNA condensates mediated efficient vitro gene transfer. Shorter polylysine chains produced larger much lower expression while longer were equivalent to AlkCWK...

N-Linked biantennary, triantennary, and core fucosylated biantennary oligosaccharides were isolated from animal glycoproteins derivatized at their reducing end with Boc-tyrosine. The terminal Gal residues enzymatically removed replaced GalNAc. Tyrosinamide-oligosaccharides radioiodinated administered intravenously to mice. Pharmacokinetic biodistribution studies revealed structure-dependent differences in the steady-state volume of distribution, total body clearance rate, targeting...