A5075453383- Cancer Genomics and Diagnostics
- Veterinary Oncology Research
- Neonatal Respiratory Health Research
- Neonatal and Maternal Infections
- Glycosylation and Glycoproteins Research
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Molecular Biology Techniques and Applications
- Congenital Diaphragmatic Hernia Studies
- Galectins and Cancer Biology
- Gene expression and cancer classification
- Streptococcal Infections and Treatments
- Infant Nutrition and Health
- Preterm Birth and Chorioamnionitis
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Education and Art Integration
- Geophysical and Geoelectrical Methods
- Helicobacter pylori-related gastroenterology studies
- Corneal Surgery and Treatments
- Geological and Tectonic Studies in Latin America
- Mine drainage and remediation techniques
University of California, San Diego
2018-2023
Autonomous University of Queretaro
2023
Rady Children's Hospital-San Diego
2019-2020
Fate Therapeutics (United States)
2020
Scripps Research Institute
2007-2016
Dendritic cells (DC) are the most potent APC in organism. Immature dendritic (iDC) reside tissue where they capture pathogens whereas mature (mDC) able to activate T lymph node. This dramatic functional change is mediated by an important genetic reprogramming. Glycosylation common form of posttranslational modification proteins and has been implicated multiple aspects immune response. To investigate involvement glycosylation changes that occur during DC maturation, we have studied...
Cancer is the leading cause of death in dogs, yet there are no established screening paradigms for early detection. Liquid biopsy methods that interrogate cancer-derived genomic alterations cell-free DNA blood being adopted multi-cancer detection human medicine and now available veterinary use. The CANcer Detection Dogs (CANDiD) study an international, multi-center clinical designed to validate performance a novel "liquid biopsy" test developed noninvasive characterization cancer dogs using...
Prior studies have shown that treatment with the peracetylated 4-fluorinated analog of glucosamine (4-F-GlcNAc) elicits anti-skin inflammatory activity by ablating N-acetyllactosamine (LacNAc), sialyl Lewis X (sLe(X)), and related lectin ligands on effector leukocytes. Based anti-sLe(X) antibody probing experiments 4-F-GlcNAc-treated leukocytes, it was hypothesized 4-F-GlcNAc inhibited sLe(X) formation incorporating into LacNAc blocking addition galactose or fucose at carbon 4-position...
Abstract Lung macrophages mature after birth, placing newborn infants, particularly those born preterm, within a unique window of susceptibility to disease. We hypothesized that in preterm lung macrophage immaturity contributes the development bronchopulmonary dysplasia (BPD), most common serious complication prematurity. By measuring changes gene expression patients at risk BPD, we show here eventually developing BPD had higher inflammatory mediator even on first day life. Surprisingly, ex...
This proof-of-concept study demonstrates that blood-based liquid biopsy using next generation sequencing of cell-free DNA can non-invasively detect multiple classes genomic alterations in dogs with cancer, including originate from spatially separated tumor sites. Eleven a variety confirmed cancer diagnoses (including localized and disseminated disease) who were scheduled for surgical resection, five presumably cancer-free dogs, enrolled. Blood was collected each subject, tissue samples...
RNA integrity is a critical factor in obtaining meaningful gene expression data. Current methodologies rely on maintaining samples cold environments during collection, transport, processing, and storage procedures, which are also extremely time-sensitive. Several products commercially available to help prevent degradation the handling steps; however, must be kept for optimal protection. We have evaluated novel medium based anhydrobiosis stabilizing protecting from at room temperature that...
Streptococcus agalactiae (Group B Streptococcus, GBS) is the most common neonatal pathogen. However, cellular and molecular mechanisms for susceptibility to GBS pneumonia sepsis are incompletely understood. Here we optimized a mouse model of test role alveolar macrophage (ΑΜΦ) maturation in host vulnerability disease. Compared with juvenile adult mice, mice infected had increased mortality persistence lung injury. In addition, were defective phagocytosis killing. ΑΜΦ depletion disruption...
The bracken fern Pteridium aquilinum is a plant known to be carcinogenic animals. Epidemiological studies have shown an association between exposure and gastric cancer development in humans. biological effects of this within the carcinogenesis process are not fully understood. In present work, mucosa mice treated with were evaluated, as well molecular mechanisms underlying synergistic role Helicobacter pylori infection. Our results showed that induces histomorphological modifications...
Abstract Distinct macrophage subsets populate the developing embryo and fetus in distinct waves. However little is known about functional differences between utero populations or how they might contribute to fetal neonatal immunity. Here we tested innate immune response of mouse macrophages derived from embryonic yolk sac liver. When isolated liver lung, CD11b HI responded TLR4 agonist LPS by expressing releasing inflammatory cytokines. F4/80 did not respond treatment. While expression...
Members of the Sp family transcription factors regulate gene expression via binding GC boxes within promoter regions. Unlike Sp1, which stimulates transcription, closely related Sp3 can either repress or activate and is required for perinatal survival in mice. Here, we use RNA-seq cellular phenotyping to show how regulates murine fetal cell differentiation proliferation. Homozygous Sp3-/- mice were smaller than wild-type Sp+/- littermates, died soon after birth had abnormal lung...
Abstract Genetic engineering of T cells using a chimeric antigen receptor targeting CD19 (CAR19) is now well-established treatment B cell malignancies. While cellular immunotherapies are entering front line treatment, substantial limitations currently hamper the broad application adoptive therapies in diverse patient population including dysfunctional starting material, lack product consistency and purity post genetic inefficient quantity produced for true on-demand availability. FT819...
Identification of novel biomarkers and targets in renal cell carcinoma (RCC) remains a priority one cellular compartment that is rich potential source such molecules the plasma membrane. A shotgun proteomic analysis surface proteins enriched by biotinylation avidin affinity chromatography was explored using UMRC2- cancer line, which lacks von Hippel-Lindau (VHL) tumour suppressor gene function, to determine whether interest could be detected. Of 814 identified ~22% were membrane or...
Abstract Group B Streptococcus (Streptococcus agalactiae, GBS) causes severe infections in neonates. While not a common adult pathogen, GBS is the leading cause of congenital pneumonia. To determine molecular mechanisms neonatal susceptibility, we developed murine pneumonia model. Neonatal, juvenile, and C57BL/6 mice were inoculated intranasally with 2800 CFU/g (COH1). After 7 d, survival was 100 % adults juveniles, 10 neonates dying within 3 d after infection. Histopathological assessments...
Abstract Group B Streptococcus (GBS) is the most common pathogen in neonates but rarely causes disease adults. GBS pneumonia newborns, suggesting a defect lung innate immunity at birth. Using novel neonatal mouse model of pneumonia, we showed that newborn mice have reduced killing and more severe persistent injury. Here test hypothesis defects alveolar macrophage (AM) maturation newborns leads to susceptibility. Clodronate depletion AM from adult prevented killing, supporting requirement for...
Abstract Group B Streptococcus (GBS) is a major neonatal pathogen but rarely causes disease in adults. We previously showed mice that GBS escapes killing the lung via its heavily sialylated capsule. Immune cells detect sialic acid moieties expression of repertoire Siglec receptors. Combinatory proinflammatory and anti-inflammatory receptors allows differentiation between host pathogenic microbial modifications. test here hypothesis alveolar macrophages (AMs) fail to kill due developmental...