A5058394258- Biomedical Ethics and Regulation
- Thermoregulation and physiological responses
- Ethics in Clinical Research
- Mesenchymal stem cell research
- Tendon Structure and Treatment
- Shoulder Injury and Treatment
- Health Systems, Economic Evaluations, Quality of Life
- Muscle metabolism and nutrition
- Periodontal Regeneration and Treatments
- Hip disorders and treatments
- Genetic Associations and Epidemiology
- Bone and Joint Diseases
- Sports injuries and prevention
- Extracellular vesicles in disease
- Musculoskeletal synovial abnormalities and treatments
- Bone fractures and treatments
- Foot and Ankle Surgery
- Orthopaedic implants and arthroplasty
- Pluripotent Stem Cells Research
- Orthopedic Surgery and Rehabilitation
- Osteoarthritis Treatment and Mechanisms
- High Altitude and Hypoxia
- Organ and Tissue Transplantation Research
- Health and Medical Research Impacts
- Cardiovascular Effects of Exercise
Mayo Clinic in Florida
2016-2025
Jacksonville College
2004-2025
WinnMed
2010-2025
Mayo Clinic
2011-2023
University of Colorado Denver
2021
Mayo Clinic in Arizona
2017-2020
Jackson Memorial Hospital
2018
GTx (United States)
2017
Jacksonville University
2009
Johnson & Johnson (United States)
2008
Concentration of extracellular vesicles (EVs) from biological fluids in a scalable and reproducible manner represents major challenge. This study reports the use tangential flow filtration (TFF) for highly efficient isolation EVs large volumes samples. When compared to ultracentrifugation (UC), which is most widely used method concentrate EVs, TFF more efficient, scalable, gentler method. Comparative assessment UC conditioned cell culture media revealed that former concentrates comparable...
Bone marrow aspirate concentrate (BMAC) is increasingly used as a regenerative therapy for musculoskeletal pathological conditions despite limited evidence-based support.BMAC will prove feasible, safe, and efficacious the treatment of pain due to mild moderate degenerative joint disease knee.Randomized controlled trial; Level evidence, 2.In this prospective, single-blind, placebo-controlled trial, 25 patients with bilateral knee from osteoarthritis were randomized receive BMAC into one...
We are conducting a genome scan at an average resolution of 10 centimorgans (cM) for type 2 diabetes susceptibility genes in 716 affected sib pairs from 477 Finnish families. To date, our best evidence linkage is on chromosome 20 with potentially separable peaks located both the long and short arms. The unweighted multipoint maximum logarithm odds score (MLS) was 3.08 20p (location, x̂ = 19.5 cM) under additive model, whereas weighted MLS 2.06 20q ( 57 cM, recurrence risk, λ̂ s 1.25, P...
OBJECTIVE To test the hypothesis that vasomotion, rhythmic contraction exhibited by small arteries and arterioles, is impaired in diabetic subjects compared with healthy control subjects. RESEARCH DESIGN AND METHODS We mathematically modeled oscillations laser Doppler microvascular measurements taken from pulpar surface of index finger 20 age-matched (8 type I 12 II diabetes). The mean duration diabetes was 17.1 ± 2.3 years, HbA1c 9.1 0.4%. Blood flow measured for 5 min as rested quietly a...
Objective Bone marrow aspiration and concentration (BMAC) is becoming a more common regenerative therapy for musculoskeletal pathology. In our current pilot study, we studied patients with mild-to-moderate bilateral knee osteoarthritis, compared pain at 12-month follow-up between BMAC-injected saline-injected knees, examined cartilage appearance measured by magnetic resonance imaging (MRI) T2 quantitative mapping. Design Twenty-five osteoarthritic were randomized to receive BMAC into one...
Abstract Extracellular vesicles secreted from adipose‐derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular (EVs) ADSCs is costly, inefficient, and time consuming. The anti‐inflammatory properties adipose tissue‐derived EVs other biogenic nanoparticles not been explored. In this study, are obtained directly lipoaspirate, an easily accessible abundant source biological material. Compared to ADSC‐EVs, lipoaspirate...
OBJECTIVE To test the hypothesis that skin blood flow responses in fingertip of diabetic patients are impaired and to examine role aging both healthy control subjects patients. RESEARCH DESIGN AND METHODS We measured cutaneous using laser Doppler techniques 40 people with diabetes 20 age- sex-matched subjects. induce vasoconstriction, were asked perform three 1-min stressor tasks: mental arithmetic, contralateral hand grip, immersion ice water. vasodilatation, a local heat stimulus 45...
Large-scale genotyping is required to generate dense identity-by-descent maps map genes for human complex disease. In some studies the number of genotypes needed can approach or even exceed 1 million. Generally, linkage and disequilibrium analyses depend on clear allele identification subsequent frequency estimation. Accurate grouping categorization each in sample (allele calling binning) therefore an absolute requirement. Hence, a system that reliably achieve this necessary. case affected...
Advancements in extracellular vesicle (EV) studies necessitate the development of optimized storage conditions to ensure preservation physical and biochemical characteristics. In this study, most common buffer for EV (phosphate-buffered saline/PBS) was compared a cryoprotective 5% sucrose solution. The size distribution concentration EVs from two different sources changed greater extent after -80 °C PBS Additionally, molecular surface protrusions transmembrane proteins were more prevalent...
Androgenic alopecia (AGA) is a common hair loss disorder. Studies have demonstrated successful treatment with platelet-rich plasma (PRP) in men, but studies women are few.To evaluate PRP the of AGA women, compared topical minoxidil.Twenty received minoxidil for 12 weeks and injectable randomized crossover design an 8-week washout between treatments. Standardized TrichoScan analysis quality-of-life questionnaires were assessed at baseline 12-week follow-up each treatment.After PRP,...