A5001486783- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Enzyme Structure and Function
- Advanced Proteomics Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Analytical chemistry methods development
- Hippo pathway signaling and YAP/TAZ
- Plant pathogens and resistance mechanisms
- Respiratory viral infections research
- Neurobiology and Insect Physiology Research
- Plant Molecular Biology Research
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- SARS-CoV-2 and COVID-19 Research
Medicago (Canada)
2023
University of Manchester
2016-2022
University of Toronto
2013-2014
Significance Bacterial pathogens can use a syringe-like structure to inject virulence proteins (effectors) directly into host cells. The YopJ/HopZ superfamily of effectors found in animal and plant modify kinase suppress immunity. In the model Arabidopsis , HopZ1a is recognized by resistance protein ZAR1 induce robust immune response that blocks pathogen growth. Here, we show effector from Pseudomonas syringae targets nonfunctional pseudokinase ZED1 required for recognition ZAR1. We...
Charge reduction in the gas phase provides a direct means of manipulating protein charge state, and when coupled to ion mobility mass spectrometry (IM-MS), it is possible monitor effect on conformation absence solution. Use electron transfer reagent 1,3-dicyanobenzene, with IM-MS, allows us two proteins deliberately chosen from opposite sides order disorder continuum: bovine pancreatic trypsin inhibitor (BPTI) beta casein. The ordered BPTI presents compact conformers for each three states...
The gas phase is an idealized laboratory for the study of protein structure, from which it possible to examine stable and transient forms mass-selected ions in absence bulk solvent. With ion mobility–mass spectrometry (IM-MS) apparatus built operate at both cryogenic elevated temperatures, we have examined conformational transitions that occur monomeric proteins: ubiquitin, lysozyme, α-synuclein as a function temperature source activation. We rationalize experimental observations with...
The Par complex is a conserved cell polarity regulator. Bazooka/Par-3 scaffold for the and contains three PDZ domains in tandem. can act singly or synergistically to bind C-termini of interacting proteins. Sequence comparisons among Drosophila Baz its human C. elegans Par-3 counterparts indicate divergence peptide binding pocket PDZ1 greater conservation pockets PDZ2 PDZ3. However, it unclear whether from different species share preferences, if their tandem organization affects properties....
The gas phase is an idealized laboratory for the study of protein structure, from which it possible to examine stable and transient forms mass selected ions in absence bulk solvent. With ion mobility-mass spectrometry (IM-MS) apparatus built operate at both cryogenic elevated temperatures, we have examined conformational transitions monomeric proteins: ubiquitin, lysozyme alpha-synuclein as a function temperature source activation. We rationalize experimental observations with dependent...
The coronavirus disease (COVID-19) pandemic shows the rapid pace at which vaccine development can occur highlights need for more fast and efficient analytical methodologies to track characterize candidate vaccines during manufacturing purification processes. in this work comprises plant-derived Norovirus-like particles (NVLPs) are structures that mimic virus but lack any infectious genetic material. Presented here is a liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodology...
<p>As experimentalists, we normally rely on assessing observables. However sometimes, the most fascinating phenomena are not noticeable directly. An example of such is our data and corresponding interpretation presented in this manuscript. We have designed constructed a ion mobility mass spectrometer (acs.analchem.6b01812) capable taking measurements over temperature range from 150-500K. chose to benchmark new instrument, using small proteins Ubiquitin Lysozyme extensively studied as...
<p>Using Ubiquitin as an exemplar protein we examine the effect of net charge reduction post electrospray ionisation by exposure to electron transfer reagent, 1,3-dicyanobenzene. We monitor change in gas phase conformation both precursor and products with ion mobility mass spectrometry (IM-MS). Dramatic conformational rearrangement is seen for low state ions upon reagent. Ions states sprayed from native-like denaturing solvent conditions undergo structural transitions conformers cross...
The coronavirus disease (COVID-19) pandemic shows the rapid pace at which vaccine development can occur highlights need for more fast and efficient analytical methodologies to track characterize candidate vaccines during manufacturing purification processes. in this work comprises plant-derived Norovirus-like particles (NVLPs) are structures that mimic virus but lack any infectious genetic material. Presented here is a liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodology...
Using Ubiquitin as an exemplar protein we examine the effect of net charge reduction post electrospray ionisation by exposure to electron transfer reagent, 1,3-dicyanobenzene. We monitor change in gas phase conformation both precursor and products with ion mobility mass spectrometry (IM-MS). Dramatic conformational rearrangement is seen for low state ions upon reagent. Ions states sprayed from native-like denaturing solvent conditions undergo structural transitions conformers cross sections...
As experimentalists, we normally rely on assessing observables. However sometimes, the most fascinating phenomena are not noticeable directly. An example of such is our data and corresponding interpretation presented in this manuscript. We have designed constructed a ion mobility mass spectrometer (acs.analchem.6b01812) capable taking measurements over temperature range from 150-500K. chose to benchmark new instrument, using small proteins Ubiquitin Lysozyme extensively studied as “model...
The gas phase is an idealized laboratory for the study of protein structure, from which it possible to examine stable and transient forms mass selected ions in absence bulk solvent. With ion mobility-mass spectrometry (IM-MS) apparatus built operate at both cryogenic elevated temperatures, we have examined conformational transitions monomeric proteins: ubiquitin, lysozyme alpha-synuclein as a function temperature source activation. We rationalize experimental observations with dependent...