A5088877512- Pancreatic function and diabetes
- Genomics and Chromatin Dynamics
- Congenital heart defects research
- Contact Dermatitis and Allergies
- Epigenetics and DNA Methylation
- Obstructive Sleep Apnea Research
- Single-cell and spatial transcriptomics
- Dermatology and Skin Diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic Associations and Epidemiology
- Allergic Rhinitis and Sensitization
- Growth Hormone and Insulin-like Growth Factors
- Genetic Syndromes and Imprinting
- Cancer, Hypoxia, and Metabolism
- Diabetes and associated disorders
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- High Altitude and Hypoxia
- Cardiac Fibrosis and Remodeling
- Genetics and Physical Performance
- Skin Protection and Aging
- Sympathectomy and Hyperhidrosis Treatments
- Mast cells and histamine
- Cancer-related gene regulation
- Proteoglycans and glycosaminoglycans research
Children's Hospital of Philadelphia
2020-2024
University Hospitals of Cleveland
1995-1996
Case Western Reserve University
1988-1996
University School
1990-1996
University Medical Center New Orleans
1992
Louisiana State University
1992
The human heart is a target organ for the octapeptide hormone, angiotensin II (Ang II). Recent studies suggest that contains dual pathway of Ang formation in which major II-forming enzymes are I-converting enzyme (ACE) and chymase. Human chymase has recently been purified its cDNA gene cloned. This cardiac serine proteinase most efficient specific described. To obtain insights into sites chymase-dependent formation, we examined cellular localization regional distribution heart. Electron...
This report concerns the relative contributions of body weight and sleep apnea to following cardiovascular risk factors: blood pressure, fasting insulin glucose. We cross-sectionally examined relationship various levels apneic activity [apnea-hypopnea index (AHI)] a measure obesity [body mass (BMI)] mean morning pressure serum glucose concentrations sampled after polysomnography. Subjects were 261 males (age 47 ± 13 years, SD), who referred laboratory for symptoms sleep-disordered breathing....
Abstract The human uterus repeatedly exhibits cyclic biochemical and cytological changes during the reproductive period of life. These are result a well‐characterized endocrine network involving hypothalamus, pituitary, ovary. exact nature mechanism(s) by which sex steroids act on remains to be elucidated. Possible local mediators hormonal action include polypeptide growth factors. Using method RNA transfer blot hybridization, we have analyzed tissue samples from cycling endometrium...
The ch12q13 locus is among the most significant childhood obesity loci identified in genome-wide association studies. This resides a non-coding region within FAIM2; thus, underlying causal variant(s) presumably influence disease susceptibility via cis-regulation. We implicated rs7132908 as putative variant by leveraging our in-house 3D genomic data and public domain datasets. Using luciferase reporter assay, we observed allele-specific cis-regulatory activity of immediate harboring...
The lymphokine interleukin 2 is an important immune system regulatory glycopolypeptide. It produced by antigen- or mitogen-stimulated T lymphocytes and required for the proliferation clonal expansion of activated lymphocytes. In this report, it demonstrated RNA transfer blot hybridization that poly(A)+ population human placenta contains a 0.85-kilobase transcript specifically hybridizes to cDNA probe. By using histochemistry in situ, further shown transcripts are localized, primarily,...
Abstract The hypothalamus regulates metabolic homeostasis by influencing behavior and endocrine systems. Given its role governing key traits, such as body weight reproductive timing, understanding the genetic regulation of hypothalamic development function could yield insights into disease pathogenesis. However, given inaccessibility, studying human gene has proven challenging. To address this gap, we generate a high-resolution chromatin architecture atlas an established embryonic stem cell...
Abstract Interleukin‐1α (IL‐1α), interleukin‐1 β (IL‐1β) and tumor necrosis factor‐α (TNF‐α) are 3 cytokines that play a key rôle in cutaneous homeostasis the immunopathogenesis of number dermatologic diseases. Most studies have focussed on their production by keratino‐cytes Langerhans cells. To determine whether there non‐epidermal sites cytokine transcription, biopsy specimens normal human skin were probed for IL‐lα, IL‐lβ TNF‐α messenger RNAs using method situ hybridization. The results...
Abstract We investigated the potential role of sleep-trait associated genetic loci in conferring a degree their effect via pancreatic α- and β-cells, given that both sleep disturbances metabolic disorders, including type 2 diabetes obesity, involve polygenic contributions complex interactions. determined commonalities between conducting linkage disequilibrium correlation analyses with publicly available GWAS summary statistics. Then we possible enrichment SNPs promoter-interacting open...
The long-term goals of our research are to understand the biochemical morbidity surrounding obstructive sleep apnea syndrome define better need for treatment and determine modifiable risk factors disease. Our current hypothesis is that sleep-related hypoxemia results in alterations metabolic regulatory peptides, specifically insulin insulin-like growth (IGF-1 IGF-2), which known or suspected obesity disorders such as hypertension, glucose intolerance, atherosclerosis. Surveys clinic...
Abstract Cell surface expression of major histocompatibility determinants on epidermal keratinocytes is a characteristic feature number inflammatory dermatoses and in all likelihood caused by diffusion human leukocyte antigen (HLA)‐DR‐inducing cytokines from cells present the dermal mononuclear cell infiltrate. Many these same disorders respond to ultraviolet(UV) radiation phototherapy. Using SCC‐13 keratinocyte line as model, UV was found inhibit interferon‐gamma‐induced HLA‐DR expression....
A primary monolayer culture system from cockerel hepatocytes was established. The cultures synthesize and secrete proteins that comigrate with authentic serum on polyacrylamide gels are found in the same relative abundance. Addition of estradiol increased synthesis apoprotein B, very low density lipoprotein, under all conditions. Vitellogenin could not be induced directly by estradiol. However, when obtained cockerels injected 4 days before blood collection included medium, secreted a...
Abstract Epidermal keratinocytes in culture have been shown to produce many cytokines, and their proteins identified skin tissue samples. It has therefore assumed that these cytokines are transcribed vivo by the epidermis response contact allergens. In this report, situ hybridization was used detect messenger RNAs for interleukin‐1 alpha (IL‐1α), beta (IL‐1β) tumour necrosis factor‐alpha (TNF‐α) samples of human prior at various times after application urushiol, immunogenic component poison...
Summary The hypothalamus regulates metabolic homeostasis by influencing behavior, energy utilization and endocrine systems. Given its role governing health-relevant traits, such as body weight reproductive timing, understanding the genetic regulation of hypothalamic development function should yield insights into these traits diseases. However, given inaccessibility, studying human gene has proven challenging. To address this gap, we generated a chromatin architecture atlas an established...
Abstract Three-dimensional (3D) chromatin organization maps help to dissect cell type-specific gene regulatory programs. Furthermore, 3D have contributed elucidating the pathogenesis of complex genetic diseases by connecting distal regions and risk variants their respective target genes. To understand architecture diabetes risk, we generated transcriptomic epigenomic profiles human pancreatic acinar, alpha, beta cells using single-cell RNA-seq, ATAC-seq, high-resolution Hi-C sorted cells....
Cytokines play an integral role in the allergic response of nasal mucosa. The ideal model for analysis this interaction has yet to be perfected. We present a such evaluation and results experiments on release several cytokines. Freshly harvested human turbinate mucosa was placed Gelfoam (Upjohn Co., Kalamazoo, Mich.) raft liquid medium simulate situ environment. initiated by exposing various combinations amounts immunoglobulin E antihuman antibody. supernatants were collected analyzed...