Sub-optimal growing conditions have a major effect on plants; therefore, large efforts are devoted to maximizing the availability of agricultural inputs crops. To increase sustainable use non-renewable inputs, attention is currently given study plants under non-optimal conditions. In this work, we investigated impact sub-optimal macrocations and light intensity in two lettuce varieties that differ for accumulation secondary metabolites (i.e., ‘Red Salanova’ ‘Green Salanova’)....

Despite the scientific evidence supporting their biostimulant activity, molecular mechanism(s) underlying activity of protein hydrolysates (PHs) and specificity among different products are still poorly explored. This work tested five hydrolysates, produced from plant sources using same enzymatic approach, for ability to promote rooting in tomato cuttings following quick dipping. Provided that all PHs increased root length (45–93%) some them number (37–56%), untargeted metabolomics followed...

A carotenoid-rich extract from Lycium barbarum L. was added to extra virgin olive oil (EVOO), obtaining a carotenoid-enriched (EVOOCar). The oxidative stability of EVOO and EVOOCar evaluated during long-term storage 28 weeks at room temperature, by measuring some classical parameters (acidity peroxide values, spectrophotometric coefficients, fatty acid composition) the content minor compounds (i.e., α-tocopherol lutein). At end storage, higher (p < 0.01) in respect were observed....

Consumption of rooibos (Aspalathus linearis) as herbal tea is growing in popularity worldwide and its health-promoting attributes are mainly ascribed to phenolic composition, which may be affected by the brewing conditions used. An aspect so far overlooked impact cold vs regular microwave boiling on poly(phenolic) profile vitro antioxidant capacity infusions prepared from red ('fermented', oxidized) green ('unfermented', unoxidized) rooibos, purpose present study. By using an untargeted...

The lentil seed coat is a waste by-product still rich in phenolic compounds, specifically condensed tannins. effect of different solvents, as well processes, namely conventional solid–liquid extraction (CSLE) and ultrasound-assisted (UAE), on the yield specific compound classes was studied. Four empirical two-parameter models were examined to select one that better fit experimental data obtained under operating conditions. Additionally, ultra-high-pressure liquid chromatography coupled...

Abstract Melatonin (MLT) is an indole derivative that exhibits hormone‐like activities in plants, regulating multiple aspects of growth and development. Due to its role mitigating oxidative stress facilitating osmoprotectant accumulation, MLT enhances abiotic tolerance, although the pathways metabolic mechanisms involved remain unclear despite being studied various crops. This work aimed investigate changes elicited by exogenous application at different concentrations (10, 50, 150 μM)...

Considering that functional components of plant foods are mainly secondary-metabolism products, we investigated the shaping health-promoting compounds in hydroponically grown butterhead lettuce (Lactuca sativa L. var. capitata) as a function strength nutrient solution utilized. To this aim, untargeted metabolomics profiling, vitro antioxidant capacity (total phenolics, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), cupric reducing...

In this work, different commercial extra-virgin olive oils (EVOO) were subjected to in vitro gastrointestinal digestion and the changes bioactive compounds evaluated by ultra-high-pressure liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry, using untargeted metabolomics. As expected, raw EVOO samples abundant total sterols (on average: 3007.4 mg equivalents/kg) tyrosol equivalents 334.1 equivalents/kg). However, UHPLC-QTOF screening allowed us annotate 309...

Abstract Biostimulants are receiving increasing attention for their beneficial effects on crops, driving interest in identifying new plant extracts that could exert such stimulatory effects. This work aimed to evaluate the potential of an aqueous extract obtained from duckweed ( Lemna minor L.), a freshwater species, act as biostimulant maize. For this purpose, plants were collected natural basin and then transferred, stabilized, grown under controlled conditions. The was first characterized...

Biostimulants are gaining increasing interest because of their ability to provide a green and effective strategy towards sustainable crop production. Nonetheless, mode action remains often unknown. The object this work was unravel the mechanisms through which 4-Vita, biostimulant plant extract, can mitigate drought stress in tomato. To aim, tomato plants were treated with two foliar applications 4-Vita imposed both control plants. Phenomics investigations coupled mass spectrometric...

Currently, there is a renewed interest towards the development of plant-based pharmacophores. In this work, 16 extracts prepared from leaves, twigs, roots and fruits hydro-halophyte, Rhizophora mucronata Lam. (Family: Rhizophoraceae), were studied for possible antioxidant activity phenolic profiles established. Thereafter, enzymatic inhibitory activities (α-amylase, α-glucosidase, tyrosinase, acetyl- (AChE), butyrylcholinesterase (BChE), lipase, elastase) assessed. The total phenolic,...

Biostimulants are becoming increasingly popular in agriculture for their ability to induce beneficial effects crops, paving the way towards identification of new materials with biostimulant potential. This study evaluated potential different concentrations an aqueous extract (0.25%, 0.50%, and 1.00%, dry weight/water volume, respectively) obtained from duckweed (Lemna minor L.) stimulate olive plants. Leaf net photosynthesis (Pn), leaf transpiration rate (E), stomatal conductance (gs),...

In this work, the phytochemical profile and biological properties of Colchicum triphyllum (an unexplored Turkish cultivar belonging to Colchicaceae) have been comprehensively investigated for first time. Herein, we focused on evaluation in vitro antioxidant enzyme inhibitory effects flower, tuber, leaf extracts, obtained using different extraction methods, namely maceration (both aqueous methanolic), infusion, Soxhlet. Besides, complete phenolic alkaloid untargeted metabolomic profiling...

Metal trace elements (MTE) can damage crops if present in excessive amounts the environment. This research investigated effect of a plant extract an aquatic species, Lemna minor L. (duckweed) (LE), on ability maize to cope with copper (Cu) toxicity. LE reversed effects Cu2+ photosynthetic activity (Pn), evapotranspiration (E), stomatal conductance (gs), sub-stomatal CO2 concentration (Ci) and biomass which did not differ from untreated controls. regulate amount leaves, but compared...

Abstract BACKGROUND In this investigation, the chemical and biological profiles of three Turkish Crataegus species ( orientalis , szovitsii tanacetifolia ) were studied in order to provide first comprehensive characterization their health‐promoting potential. respect, polyphenolic evaluated using ultrahigh‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry. The vitro antioxidant activities samples by free‐radical scavenging, phosphomolybdenum, ferrous‐ion...

The quest for sustainable strategies aimed at increasing the bioactive properties of plant-based foods has grown quickly. In this work, we investigated impact exogenously applied phenolics, i.e., chlorogenic acid (CGA), hesperidin (HES), and their combinations (HES + CGA), on Lactuca sativa L. under normal- mild-salinity conditions. To aim, phenolic profile, antioxidant properties, enzyme inhibitory activity were determined. untargeted metabolomics profiling revealed that lettuce treated...

Bacterial production of conjugated linoleic acid (CLA) has recently received great attention because the potential health benefits this fatty acid. Linoleic (LA) can be converted to CLA by several microorganisms, including bifidobacteria, possibly as a detoxification mechanism avoid growth inhibition effect LA. In present in vitro study, we investigated gene expression landscape intestinal strain Bifidobacterium breve DSM 20213 when exposed Transcriptomic analysis using RNA-seq revealed that...