A5038394348- Microfluidic and Capillary Electrophoresis Applications
- Protein purification and stability
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanopore and Nanochannel Transport Studies
- Monoclonal and Polyclonal Antibodies Research
- Microbial Community Ecology and Physiology
- Cannabis and Cannabinoid Research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Fluid Dynamics and Mixing
- Bacterial biofilms and quorum sensing
- Neuroscience and Neuropharmacology Research
- thermodynamics and calorimetric analyses
- Bacteriophages and microbial interactions
- Electrowetting and Microfluidic Technologies
- Clostridium difficile and Clostridium perfringens research
- Analytical Chemistry and Chromatography
- GABA and Rice Research
- Genomics and Phylogenetic Studies
Biogen (United States)
2020-2022
Indiana University Bloomington
2012-2016
RTI International
2012
Women's Health Research Institute
2007
ABSTRACT The dimorphic alphaproteobacterium Prosthecomicrobium hirschii has both short-stalked and long-stalked morphotypes. Notably, these morphologies do not arise from transitions in a cell cycle. Instead, the maternal morphology is typically reproduced daughter cells, which results microcolonies of single type. In this work, we further characterized cells found that have Caulobacter -like life cycle division leads to generation two morphologically distinct cells. Using microfluidic...
An international team spanning 19 sites across 18 biopharmaceutical and in vitro diagnostics companies the United States, Europe, China, along with one regulatory agency, was formed to compare precision robustness of imaged CIEF (ICIEF) for charge heterogeneity analysis National Institute Standards Technology (NIST) mAb a rhPD-L1-Fc fusion protein on iCE3 Maurice instruments. This information has been requested help better understand how these instruments transition ICIEF methods from...
We describe a microfluidic device with an integrated nanochannel array to trap individual bacteria and monitor growth reproduction of lineages over multiple generations. Our poly(dimethylsiloxane) comprises pneumatically actuated that includes 1280 channels widths from 600 1000 nm actively diverse bacteria. Integrated pumps valves perform on-chip fluid cell manipulations provide dynamic control loading nutrient flow, permitting chemostatic for extended periods time (typically 12 20 h)....
We report the development of an automated microfluidic "baby machine" to synchronize bacterium Caulobacter crescentus on-chip and move synchronized populations downstream for analysis. The device is fabricated from three layers poly(dimethylsiloxane) has integrated pumps valves control movement cells media. This synchronization method decreases incubation time media consumption improves synchrony quality compared conventional plate-release technique. Synchronized are collected at intervals...
Therapeutic monoclonal antibodies (mAbs) are highly heterogeneous as a result of posttranslational modifications (PTMs) during bioprocessing and storage. The that impact mAb product quality regarded critical attributes require monitoring. conventional LC-mass spectrometer (MS) method used for monitoring may protein A purification prior to analysis. In this paper, we present high-throughput microchip electrophoresis (<4 min) in-line with MS (MCE-MS) enables baseline separation...
Residence time distribution modeling of integrated perfusion to capture process can elucidate the impact product quality excursions and filter fouling on monoclonal antibody production. In this case study, a glycosylation inhibitor fluorescently labeled are applied continuous study protein modulation, fouling, unit operation hold times. The operations were modeled as continuous-stirred tank reactors residence small molecule glycan characterized. A was tracer confirmed packed cell volume...