A5010321945- Respiratory viral infections research
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- RNA Interference and Gene Delivery
- Neonatal Respiratory Health Research
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Heart Failure Treatment and Management
- Advanced Drug Delivery Systems
- Legume Nitrogen Fixing Symbiosis
- Animal Virus Infections Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Congenital Diaphragmatic Hernia Studies
- interferon and immune responses
- Pediatric health and respiratory diseases
- Plant Stress Responses and Tolerance
- Dermatology and Skin Diseases
- Inhalation and Respiratory Drug Delivery
University of South Florida
2015-2024
James A. Haley Veterans' Hospital
2015-2024
United States Department of Veterans Affairs
2022
Florida College
2008-2019
Bidhan Chandra Krishi Viswavidyalaya
2019
University of Tampa
2007-2018
Nanomed (Brazil)
2015
Universidad Simón Bolívar
2012
University of Cartagena
2012
Universidad del Norte
2012
To demonstrate the involvement of tobacco smoking in pathophysiology lung disease, responses pulmonary epithelial cells to cigarette smoke condensate (CSC) — particulate fraction were examined. The human alveolar cell line A549 and normal bronchial (NHBEs) exposed 0.4 μg/ml CSC, a concentration that resulted >90% survival <5% apoptosis. Changes gene expression signaling determined by RT-PCR, western blotting immunocytofluorescence. NHBEs CSC showed increased inflammatory mediators sICAM-1,...
The potential of cationic SiO2 nanoparticles was investigated for in vivo gene transfer this study. Cationic with surface modification were generated using amino-hexyl-amino-propyltri-methoxysilane (AHAPS). zeta the at pH = 7.4 varied from -31.4 mV (unmodified particles; 10 nm) to +9.6 (modified by AHAPS). Complete immobilization DNA nanoparticle achieved a particle ratio 80 (w/w nanoparticle/DNA ratio). modified had size 42 nm distribution 10-100 nm. ability these particles transfect...
Combining chemotherapy with gene therapy has been one of the most promising strategies for treatment cancer. Noninvasive MRI superparamagnetic iron oxide (SPIO) as a contrast agent is effective techniques evaluating antitumor therapy. However, constructing single system that can deliver efficiently gene, drug and SPIO to cancer site remains challenge. Herein, we report chitosan functionalized magnetic graphene (CMG) nanoparticle platform simultaneous gene/drug delivery tumor. The phantom ex...
Cold-acclimation-specific (CAS) gene expression has been examined by screening a cDNA library prepared from poly(A)(+) RNA of cold-acclimated seedlings freezing-tolerant variety alfalfa (Medicago falcata cv Anik). Three CAS clones, pSM784, pSM2201, and pSM2358, representing different sequence species, have used to investigate the relative abundance time-course accumulation corresponding transcripts. Results obtained show that these genes is regulated in coordinated manner most likely at...
The development of a suitable three dimensional (3D) culture system for anticancer drug remains an unmet need. Despite progress, simple, rapid, scalable and inexpensive 3D-tumor model that recapitulates in vivo tumorigenesis is lacking. Herein, we report on the characterization 3D nanofibrous scaffold produced by electrospinning mixture poly(lactic-co-glycolic acid) (PLGA) block copolymer polylactic acid (PLA) mono-methoxypolyethylene glycol (mPEG) designated as 3P. Cancer cells cultured 3P...
The spike proteins of enveloped viruses are transmembrane glycoproteins that typically undergo post-translational attachment palmitate on cysteine residues the cytoplasmic facing tail protein. role protein palmitoylation in virus biogenesis and infectivity is being actively studied as a potential target novel antivirals. Here, we report first five C-terminal cysteine-rich domain SARS-CoV-2 S indispensable for infection, palmitoylation-deficient mutants defective membrane fusion. DHHC9...
Respiratory syncytial virus (RSV) infection is often associated in infancy with life-threatening bronchiolitis, which also a major risk factor for the development of asthma. At present, no effective prophylaxis available against RSV infection. Herein, we describe an prophylactic intranasal gene transfer strategy utilizing chitosan-DNA nanospheres (IGT), containing cocktail plasmid DNAs encoding all antigens, except L. A single administration IGT (25 μg/mouse) induces expression mRNA and...
The hyaluronan (HA) receptor RHAMM is an important regulator of cell growth. Overexpression transforming and required for H-ras transformation. molecular mechanism underlying growth control by other extracellular matrix receptors remains largely unknown. We report that soluble induces G2/M arrest suppressing the expression Cdc2/Cyclin B1, a protein kinase complex essential mitosis. Down-regulation use dominant negative mutants or antisense mRNA also decreases Cdc2 levels. Suppression occurs...
A comparison of abscisic acid (ABA)-induced and cold-acclimation-induced freezing tolerance in two alfalfa cultivars (Medicago falcata cv Anik Medicago sativa v Trek) indicates that ABA alone can increase to some extent, but for the development maximum tolerance, cold acclimation is essential. Analysis vivo-labeled proteins ABA-treated seedlings reveals causes several changes pattern protein synthesis. While these appear be similar those induced by acclimation, others seem specific...
Changes in the rate and pattern of protein synthesis translatable mRNA population during cold acclimation alfalfa (Medicago falcata cv Anik) seedlings have been examined. There appears to be a positive correlation between increase ability synthesize proteins at 4 degrees C freezing resistance (survival -10 C). Results obtained with three different approaches using sodium dodecyl sulfate-polyacrylamide gel electrophoresis visualized by (a) staining, (b) immunoblotting autoradiography, (c)...
Abstract Background Chitosan, a polymer derived from chitin, has been used for nasal drug delivery because of its biocompatibility, biodegradability and bioadhesiveness. Theophylline is that reduces the inflammatory effects allergic asthma but difficult to administer at an appropriate dosage without causing adverse side effects. It was hypothesized adsorption theophylline chitosan nanoparticles modified by addition thiol groups would improve absorption bronchial epithelium enhance...
Respiratory syncytial virus (RSV) nonstructural protein 1(NS1) attenuates type-I interferon (IFN) production during RSV infection; however the precise role of NS1 in orchestrating early host-virus interaction infection is poorly understood. Since constitutes first gene transcribed and IFN depends upon RLR (RIG-I-like receptor) signaling, we reasoned that may interfere with this signaling. Herein, report localized to mitochondria binds mitochondrial antiviral signaling (MAVS). Live-cell...
Graphene, with its exceptional mechanical, thermal, electrical, optical, and electronic properties may exist as single-, few-, or multilayered thin sheets. Though the number of layers affect conductivity, role toward enzyme immobilization enzymatic biosensing applications is unknown. Herein, we report electrochemical performance electrodes graphene comprising varying immobilized glucose oxidase for detection model analyte glucose. It interesting to note that these different-layered exhibit...
Respiratory syncytial virus (RSV) infection accounts for approximately 64 million cases of respiratory disease and 200,000 deaths worldwide each year, yet no broadly effective prophylactic or treatment regimen is available. RSV deploys paired, self-associating, heptad repeat domains its fusion protein, RSV-F, to form a fusogenic 6-helix bundle that enables the penetrate host cell membrane. Here, we developed hydrocarbon double-stapled peptides exhibit stabilized α-helical structure striking...