A5019964657- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Lipid Membrane Structure and Behavior
- Mosquito-borne diseases and control
- Glycogen Storage Diseases and Myoclonus
- Plant-Microbe Interactions and Immunity
- Virus-based gene therapy research
- Nuclear Structure and Function
- Antifungal resistance and susceptibility
- Fungal Biology and Applications
- Lysosomal Storage Disorders Research
- Genetics and Neurodevelopmental Disorders
- Fusion materials and technologies
- Superconductivity in MgB2 and Alloys
- Caveolin-1 and cellular processes
- Nuclear Materials and Properties
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Animal Genetics and Reproduction
- Viral Infections and Immunology Research
- Biomedical Research and Pathophysiology
- interferon and immune responses
- Insect symbiosis and bacterial influences
- Erythrocyte Function and Pathophysiology
Tel Aviv University
2006-2024
Exact Sciences (United States)
2024
Bar-Ilan University
2011-2012
COPII and COPI mediate the formation of membrane vesicles translocating in opposite directions within secretory pathway. Live-cell electron microscopy revealed a novel mode function for during cargo export from ER. is recruited to membranes defining boundary between ER exit sites, facilitating selective concentration. Using direct observation living cells, we monitored selection processes, accumulation, fission COPII-free ERES membranes. CRISPR/Cas12a tagging, RUSH system, pharmaceutical...
Zika virus (ZIKV) is an arthropod-borne emerging pathogen causing febrile illness. ZIKV associated Guillain-Barré syndrome and other neurological complications. Infection during pregnancy with complications developmental abnormalities collectively defined as congenital syndrome. There still no vaccine or specific treatment for infection. To identify host factors that can rescue cells from infection, we used a genome-scale CRISPR activation screen. Our highly ranking hits included short list...
Abstract Echinocandins are a class of antifungal drugs that inhibit the activity β‐(1,3)‐glucan synthase complex, which synthesizes fungal cell wall β‐(1,3)‐glucan. Echinocandin resistance is linked to mutations in FKS gene, encodes catalytic subunit glucan complex. We present molecular‐docking‐based model provides insight into how echinocandins interact with target Fks protein: form ternary complex both and membrane lipids. used reductive dehydration alcohols generate dehydroxylated...
Lipid droplets (LDs) comprise a triglyceride core surrounded by lipid monolayer enriched with proteins, many of which function in LD homeostasis. How proteins are targeted to the growing is still unclear. Rab1b, GTPase regulating secretory transport, was recently associated targeting LDs Drosophila RNAi screen. formation prevented human hepatoma cells overexpressing dominant-negative Rab1b. We thus hypothesized that Rab1b recruits lipid-synthesizing enzymes, facilitating growth. Here, FRET...
Article6 September 2021Open Access Source DataTransparent process Alleviation of a polyglucosan storage disorder by enhancement autophagic glycogen catabolism Or Kakhlon Corresponding Author [email protected] orcid.org/0000-0001-7435-6454 Department Neurology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel Search for more papers this author Hilla Vaknin orcid.org/0000-0001-9101-1423 Laboratory Neurodegenerative Diseases and Personalized Medicine, The Cell Screening Facility...
The cellular destination of secretory proteins is determined by interactions their targeting motifs with coat-protein complexes. transmembrane domain (TMD) also plays a central role in transport and targeting. However, comprehensive model that considers both TMD- targeting-sequence-mediated has never been advanced. We focused on the two fluorescently tagged membrane proteins: vesicular stomatitis virus G tsO45 (VSVG), which cargo protein thermoreversible mutant, Golgi-resident GalT-CFP. A...
Pluripotent human stem cells are a powerful tool for the generation of differentiated that can be used study disease. We recently demonstrated neurons derived from pluripotent embryonic (hESC) infected by highly host-restricted alphaherpesvirus varicella-zoster virus (VZV), permitting interaction VZV with to readily evaluated in culture. In present study, we examine whether hESC and neural progenitors at intermediate stages differentiation permissive infection. demonstrate here infection is...
Abstract Echinocandins are a class of antifungal drugs that inhibit the activity β‐(1,3)‐glucan synthase complex, which synthesizes fungal cell wall β‐(1,3)‐glucan. Echinocandin resistance is linked to mutations in FKS gene, encodes catalytic subunit glucan complex. We present molecular‐docking‐based model provides insight into how echinocandins interact with target Fks protein: form ternary complex both and membrane lipids. used reductive dehydration alcohols generate dehydroxylated...
The sorting and concentration of cargo proteins within ER exit sites (ERESs) is a fundamental function the secretory machinery. mechanism by which peripheral coat complexes their small GTPase effectors mediate this with export membrane domains only partially understood. secretory-machinery-mediated to ERESs process that counters entropy-driven even distribution organellar membranes. Here, for first time, we quantified dynamic properties GFP-VSVG in living cells uncoupling it from later...
Viperin is a multifunctional interferon-inducible broad-spectrum antiviral protein. belongs to the S-Adenosylmethionine (SAM) superfamily of enzymes known catalyze wide variety radical-mediated reactions. However, exact mechanism by which viperin exerts its functions still unclear. Interestingly, for many RNA viruses was shown inhibit viral accumulation interacting with different non-structural proteins. Here, we show that inhibits synthesis bacteriophage T7 polymerase in mammalian cells....
Differential solubilization of membrane components by cold 1% Triton X-100 extraction is common practice in cell biology and research, used to define of, or localization within domains called lipid rafts. In this study, biological membranes was continuously monitored single cells confocal microscopy. The distributions fluorescently-tagged proteins that label raft non-raft membranes, cytosolic cytoskeletal were upon addition the detergent. Membranes containing protein VSVG-GFP immediately...
Summary COPII and COPI are considered to be analogous sets of vesicle coat protein heterocomplexes. Coupled cargo selection, they mediate the formation membrane vesicles translocating in opposite directions differ rent destinations within secretory pathway. Here, live cell electron microscopy provided evidence for a different localization mode function during export from endoplasmic reticulum (ER). Pharmaceutical genetic perturbations ER-Golgi transport were used demonstrate that is...
Sandfly fever viruses are emerging Phleboviruses typically causing mild febrile illness. Some strains, however, can cause severe and occasionally fatal neuro-invasive disease. Like most viruses, have devised various strategies to inhibit the type I interferon (IFN) response support a productive infection. Still, of identified so far focus on inhibiting sensing arm IFN response. In contrast, effect sandfly virus infection signaling from receptor is less characterized. Therefore, we tested...
Detection of grafted human cells in mice using fluorescence is a rapid and simple technique whose use continually expanding. Robust engraftment hematological malignancy (HHM) lines patient into the naturally immunodeficient turkey embryo has recently been demonstrated by polymerase chain reaction (PCR), activated cell sorting (FACS) histology. We demonstrate here that imaging for detecting homing derived from HHM embryos. Raji lymphoma expressing far-red fluorescent protein were injected...
Zika virus (ZIKV) is an arthropod-borne emerging pathogen causing febrile illness. ZIKV associated with the Guillain–Barré syndrome and other neurological complications. The vertical transmission of can cause fetus demise, stillbirths or severe congenital abnormalities There still no vaccine specific treatment for infection. To identify host factors that rescue cells from infection, we used a genome-scale CRISPR activation screen. Our highly ranking hits included short list...
COPII and COPI are considered to be analogous sets of vesicle coat protein hretrocomplexes. Coupled cargo selection, they mediate the formation membrane vesicles translocating in opposite directions different destinations within secretory pathway. Here, live cell electron microscopy provided evidence for a localization mode function during export from endoplasmic reticulum (ER). Pharmaceutical genetic perturbations ER-Golgi transport were used demonstrate that is recruited membranes defining...
Abstract This work employs Adult Polyglucosan Body Disease (APBD) models to explore the efficacy and mechanism of action 144DG11, a new polyglucosan-reducing lead compound discovered by high-throughput screen (HTS). APBD is an adult onset glycogen storage disorder (GSD) manifesting as debilitating progressive axonopathic leukodystrophy. caused branching enzyme (GBE) deficiency leading poorly branched insoluble inclusions, which precipitate neuropathogenic polyglucosans (PG). 144DG11 led...