Human induced pluripotent stem cells (hiPSCs) are an important cell source for regenerative medicine products. Effective methods of preservation critical to their clinical and commercial applications. The use a dimethyl sulfoxide (DMSO)-free solution containing all nontoxic molecules offers effective alternative the conventional DMSO alleviates pain points associated with in cryopreservation hiPSCs. Both hiPSCs differentiated from them commonly multicellular systems, which more sensitive...

The objective of this investigation was to demonstrate the effectiveness a tissue-engineered collagen sponge as substrate for culture human corneal cells. To that end, kerotocyte, epithelial, and endothelial cells were cultured separately on sponges composed native fibrillar with pore size approximately 0.1 mm. Co-culture experiments also performed (epithelial/endothelial epithelial/keratocyte cultures). Proliferation keratocytes matrix production assessed. morphology epithelial cell...

Amid decades of research, the basic mechanisms lyo-/cryostabilization proteins and more complex organisms have not yet been fully established. One major bottleneck is inability to probe into control molecular level interactions. The interactions are responsible for significant differences in outcome preservation processes.(1) In this communication, we utilized confocal Raman microspectroscopy quantify freezing-induced microheterogeneity phase separation (solid liquid) a frozen solution...

The growth in refractive surgeries and corneal replacements has fueled interest the development of a tissue-engineered cornea. This study characterizes microstructure biomechanical properties film-based stroma equivalents over time culture. increased collagen density films was hypothesized to result improved mechanical both initially time. stromal equivalent examined using atomic force microscopy scanning electron microscopy; properties, relaxed modulus, ultimate tensile strength were...

BACKGROUND: Cryopreservation of hematopoietic progenitor cells using liquid nitrogen and controlled‐rate freezing requires complex equipment highly trained staff is expensive. We compared the method with methods a combination dimethyl sulfoxide (DMSO) hydroxyethyl starch (HES) for cryopreservation followed by storage in mechanical freezers. STUDY DESIGN AND METHODS: Peripheral blood stem (PBSCs) were collected from normal donors apheresis allocated to one four preservation conditions: 1) 10%...

There is demand for non-dimethyl sulfoxide (DMSO) cryoprotective agents that maintain cell viability without causing poor postthaw function or systemic toxicity. The focus of this investigation involves expanding our understanding multicomponent osmolyte solutions and their ability to preserve during freezing. Controlled cooling rate freezing, Raman microscopy, differential scanning calorimetry (DSC) were utilized evaluate the differences in recovery ice crystal formation behavior containing...

Inadequate preservation methods of human induced pluripotent stem cells (hiPSCs) have impeded efficient reestablishment cell culture after the freeze-thaw process. In this study, we examined roles cooling rate, seeding temperature, and difference between aggregates (3-50 cells) single in controlled rate freezing hiPSCs. Intracellular ice formation (IIF), post-thaw membrane integrity, attachment, apoptosis, cytoskeleton organization were evaluated to understand different responses hiPSC...

Adult stem cells (hematopoietic and mesenchymal) have demonstrated tremendous human therapeutic potential. Currently, embryonic are used principally for understanding development disease progression but also hold The ability to preserve is critical their use in clinical research applications. Preservation of permits the transportation between sites, as well completion safety quality control testing. a 'manufacturing paradigm' cell therapies, thereby maximizing number products that can be...

Current methods for freezing mesenchymal stromal cells (MSCs) result in poor post-thaw function, which limits the clinical utility of these cells. This investigation develops a novel approach to preserve MSCs using combinations sugars, sugar alcohols, and small-molecule additives. frozen solutions exhibit improved attachment more normal alignment actin cytoskeleton compared exposed dimethylsulfoxide (DMSO). Osteogenic chondrogenic differentiation assays show that retain their lineage...

There is considerable interest in the use of sugars to preserve cells. In this study, low temperature Raman spectroscopy was used characterize behaviors sucrose during freezing. The hydrogen bond network between and water investigated at −10 °C −50 °C, spectra showed strengthened sucrose–water sucrose–sucrose bonds more concentrated solution °C. concentration ice interface increased as density decreased, it plateaued across a narrow channel nonfrozen before decreased toward next interface....

<title>Abstract</title> Background Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have attracted significant interest for use in disease modeling, drug discovery and potential therapeutic applications. However, conventional hiPSC-CM cryopreservation protocols largely dimethyl sulfoxide (DMSO) as the cryoprotectant (CPA), which is linked with a loss of post-thaw recovery function various cell types not ideal protocols. Additionally, effect freezing parameters such...

Cell matrix interactions are important in understanding the healing characteristics of cornea after refractive surgery or transplantation. The purpose this study was to characterize more detail evolution biomechanical and optical properties a stromal equivalent (stromal fibroblasts cultured collagen matrix). Human corneal were matrix. Compaction modulus determined for as function time culture composition. stained α-smooth muscle actin expression an indicator myofibroblast phenotype. nominal...

This investigation describes the use of a differential evolution (DE) algorithm to optimize cryopreservation solution compositions and cooling rates for specific cell types. Jurkat cells (a lymphocyte model type) mesenchymal stem (MSCs) were combined with non-DMSO solutions at concentrations dictated by DE algorithm. The then frozen in 96-well plates algorithm-dictated range 0.5–10°C/min. was iterated until convergence resulted identification an optimum composition rate, which occurred...