Evaluation of the nuclear to cytoplasmic ratio is commonly used for assessment presence malignancy and grading typing malignant neoplasms. Despite its widespread usage, little information exists regarding accuracy reproducibility non-automated assessment.Forty-seven cells obtained from Papanicolaou stained urine cytologies were assessed by quantitative image analysis area cell area. The nuclear/cytoplasmic was calculated. Visual estimates N/C made six pathologists. Statistical performed...

Background: The Paris System for Reporting Urinary Cytology represents a significant improvement in classification of urinary specimens. system acknowledges the difficulty cytologically diagnosing low-grade urothelial carcinomas and has developed categories to deal with this issue. uses six categories: unsatisfactory, negative high-grade carcinoma (NHGUC), atypical cells, suspicious carcinoma, other malignancies seventh subcategory (low-grade neoplasm). Methods: Three hundred fifty-seven...

In 2024, the World Health Organization (WHO) is scheduled to publish WHO Reporting System for Soft Tissue Cytopathology (WHORSSTC). This system establishes categories with well-defined definitions, criteria, and estimated risks of malignancy (ROMs) soft tissue tumors. The estimates ROM are based on a relatively small number published studies. Interobserver reproducibility not addressed in reporting even though highly important.

ABSTRACT Background Endobronchial ultrasound guided Transbronchial Needle Aspiration (EBUS‐TBNA) is the predominant method for investigation of centrally located solitary pulmonary nodules. The associated with good to excellent diagnostic sensitivity and specificity positive predictive value test reaching 100% reported negative values FNA nodules ranging from 53% 97%. impact correlating cytologic results imaging clinical findings improvement has been poorly studied. Materials Methods An...

ABSTRACT Background Up to 30% of thyroid nodules, by fine needle aspiration (FNA), have indeterminate cytology, including Bethesda III (atypia undetermined significance [AUS]), IV (follicular neoplasm/suspicious for follicular neoplasm [FN/SFN]), and V (suspicious malignancy). Molecular testing is utilized further risk stratification these nodules. A multiplatform test (MPTX), one the commercially offered molecular tests, combines next‐generation sequencing panel (ThyGeNEXT) with microRNA...

Abstract Two novel, bionanocomposite scaffolds were evaluated in a rodent model over the course of three months to determine whether these possessed adequate biocompatibility characteristics warrant further evaluation as possible tissue reconstruction scaffolds. These comprised amine‐functionalized gold nanoparticles (AuNP) or silicon carbide nanowires (SiCNW) crosslinked an acellular porcine diaphragm tendon. It was hypothesized that addition nanomaterials tendon would also improve its by...

Abstract Background In 2018, the Milan System for Reporting Salivary Gland Cytopathology was published defining a diagnostic categorization scheme with known malignancy risks and clinical follow up recommendations. Inter‐observer reproducibility of categories not defined. Methods gland fine‐needle aspirations (FNA) were reviewed over 5 year period classified by three independent observers. estimated using observed agreement chance corrected (Cohen's kappa). Results Four hundred eight cases...

Introduction: The Papanicolaou Society of Cytopathology (PSC) has developed a set guidelines for reporting respiratory cytology. While the malignancy risk each category is known, interobserver reproducibility these diagnostic categories not been well described. Methods: Fifty-five cytologic specimens obtained by fine needle aspiration from pulmonary nodules were independently reviewed four board-certified cytopathologists and assigned to described PSC specimens. Statistical analysis accuracy...

In 2016, the Papanicolaou Society of Cytopathology (PSC) proposed a classification scheme for reporting cytologic specimens obtained from respiratory system. Diagnostic sensitivity, specificity, and risk malignancy were reported endobronchial ultrasound guided fine needle aspiration but data other sampling techniques has been poorly documented.In modified version PSC guidelines was adopted at University Missouri sputum, bronchial washing, brushing, fine-needle specimens. Specimens assigned...

Background The separation of branchial cleft cysts from metastatic cystic squamous cell carcinomas in adults can be clinically and cytologically challenging. Diagnostic accuracy for is reported to as low 75% prompting some authors recommend frozen section evaluation suspected before resection. We evaluated 19 cytologic features determine which were useful this distinction. Methods Thirty‐three cases (21 carcinoma 12 cysts) histologically confirmed lesions the lateral neck graded presence or...

TSPYL5, a putative tumor suppressor gene, belongs to the nucleosome assembly protein family. The chromosomal location of TSPYL5 gene is 8Q22.1, and its exact role in prostate cancer etiology remains unclear. Further expression carcinoma cells diseased tissues including susceptibility for epigenetic silencing unknown. Also, not known variation with regards progression prostatic possible drug sensitivity.TSPYL5, DNMT-1 DNMT-B DU145, LNCaP RWPE-1 was analyzed by qRT-PCR RT-PCR. Demethylation...

The Papanicolaou Society of Cytopathology has developed a set guidelines which include diagnostic scheme with the categories "atypical" and "suspicious for malignancy." These intermediate may help stratify risk malignancy samples obtained from bile pancreatic ducts. However, reproducibility these is currently unknown.Twenty sequential brushing specimens or ducts each malignancy" were identified slides retrieved. All 40 cases reviewed independently by four cytopathologists blinded to original...

Background Fine‐needle aspiration (FNA) of a neck mass is frequently the initial diagnostic procedure for patients with human papillomavirus–positive head and squamous cell carcinoma. By performing p16 immunocytochemistry (ICC) stain on FNA material, pathologist can help to direct treating physician's search primary site select proper management patient. There currently no established threshold evaluation ICC in cytology samples. This study was aimed at establishing an optimal interpretation...

<b><i>Background:</i></b> A number of guidelines have been developed to improve standardization the terminology and criteria for cytologic specimens obtained from thyroid, pancreas, lung, salivary glands. major goal these is reproducibility understanding reporting diagnostic results among cytopathologists between clinicians. The International Academy Cytology Yokohama System Reporting Breast Fine-Needle Aspiration Biopsy Cytopathology (IAC YSRB) most recent...

A number of categorization systems had been developed for the reporting cytology specimens with aim providing uniform definitions, criteria, and diagnostic terminology. The intention these is to improve reproducibility standardized estimates malignancy risk. Required success a high level interobserver category assignment. Recently, international system serous fluid cytopathology (TIS) was proposed using categories nondiagnostic, negative malignancy, atypia undetermined significance (AUS),...

To determine the impact of consensus conferences on frequency discrepant cases in a surgical pathology practice.The percentage discrepancies reviewed at weekly conference was calculated for first and last months 13-month period. Both interrater agreement with diagnoses were assessed. A total 309 performed month 518 month. absolute chance-corrected each period.Absolute rate increased from 91.2% to 98.2% final Chance-corrected 0.80 0.97 month.The technique appears be useful method reduce...