A5017642797- Marine Bivalve and Aquaculture Studies
- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- Genetic diversity and population structure
- Marine and fisheries research
- Aquatic Invertebrate Ecology and Behavior
- Calcium Carbonate Crystallization and Inhibition
- Fish Ecology and Management Studies
- Parasite Biology and Host Interactions
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Reproductive Biology and Fertility
- Crustacean biology and ecology
- Marine Biology and Environmental Chemistry
- Cephalopods and Marine Biology
- Ocean Acidification Effects and Responses
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Isotope Analysis in Ecology
- Genetic and phenotypic traits in livestock
- Environmental DNA in Biodiversity Studies
- Entomological Studies and Ecology
- Genetic Syndromes and Imprinting
- Mollusks and Parasites Studies
Ocean University of China
2013-2024
CAE (Canada)
2001-2002
Abstract This study represents the first comprehensive molecular assessment of northwestern Pacific molluscs. In total, 2801 DNA barcodes belonging to 569 species from China, Japan and Korea were analyzed. An overlap between intra- interspecific genetic distances was present in 71 species. We tested efficacy this library by simulating a sequence-based specimen identification scenario using Best Match (BM), Close (BCM) All Species Barcode (ASB) criteria with three threshold values. BM...
Seasonal variations in oestradiol-17β and testosterone levels of the razor clam Sinonovacula constricta from Yellow River delta China were investigated relation to reproductive cycle April 2007 March 2008. Histological analysis indicated that gametogenesis began June–July when water temperature was 22.0–27.0°C ended during September–October food abundant. Oestradiol-17β concentrations determined by enzyme-linked immunosorbent assay gonad S. constricta. concentration female gonads higher than...
A sudden drop in salinity following extreme precipitation events usually causes mass mortality of oysters exposed to pathogens ocean environment. While how low stress interacts with cause remains obscure. In this study, we performed an experiment by and pathogen infection Vibrio alginolyticus investigate their synergistic effect on the Pacific oyster toward understanding interaction among environment, host, pathogen. We showed that did not significantly affect proliferation virulence V. ,...
Interspecific hybridization is a useful tool in genetic improvement of agriculture and aquaculture species. The Pacific oyster (Crassostrea gigas) the Hong Kong hongkongensis) are both important species China. To determine whether these 2 can hybridize produce viable offspring, we conducted 2×2 factorial crosses between them. Asymmetry fertilization was observed when C. hongkongensis eggs were fertilized readily by gigas sperm, but reciprocal cross resulted no fertilization. Embryos from...
Hybrid oysters often show heterosis in growth rate, weight, survival and adaptability to extremes of salinity. Oysters have also been used as model organisms study the evolution host-defense system. To gain comprehensive knowledge about various physiological processes hybrid under low salinity stress, we performed transcriptomic analysis gill tissue Crassostrea sikamea ♀ × angulata♂ using deep-sequencing platform Illumina HiSeq. We exploited high-throughput technique delineate differentially...
Abstract The reproductive cycle and seasonal variations in biochemical composition of the razor clam Sinonovacula constricta (Lamarck) from Yellow River delta China were investigated April 2007 to March 2008 relation environmental factors. Histological analysis indicated that gametogenesis S. comprised two phases: a resting phase (February–May) gametogenesis, including ripeness spawning, during rest year. Gametogenesis began June–July was completed September–October when food abundant. A...
The genetic diversity of cultured populations the Pacific abalone (Haliotis discus hannai Ino) from northern China was analysed using seven microsatellite markers. loci were polymorphic for all populations, with an average 8.7 alleles per locus (range 8.0–9.4). mean observed and expected heterozygosities 0.547 0.500–0.596) 0.774 0.754–0.787) respectively. allelic in terms number considerably lower than that previously found wild 21.8–23.0), indicating bottleneck effects occurred when each...
The Kumamoto oyster (Crassostrea sikamea) and the Portuguese (C. angulata) are important aquaculture species which naturally coexist along southern coast of China. To understand potential feasibility hybridization between two species, we conducted two-by-two factorial cross-experiments in Beihai (Guangxi province), also compared survival growth hybrids to that parental progenies during grow-out period from July 2014 2015. Genetic analysis confirmed hybrid spats were true hybrids....
Testis-specific serine/threonine kinase 1 (Tssk1) was named since its function determined in mouse spermiogenesis. In our research, we cloned a homolog of the pen shell Atrina pectinata Tssk1, ApTssk1, and expression characteristics at mRNA. The full length ApTssk1 cDNA 1517 bp, with an open reading frame 1089 bp (58–1146), which encodes peptide including 362 amino acids. homologous analysis indicated that deduced acid sequences shared close identity other reported Tssk1. addition, highly...
Abstract Seven high polymorphic microsatellite loci were used to determine the pedigrees in a mass spawning of Pacific oyster, Crassostrea gigas , and estimate genetic variability between broodstock offspring. Parental assignment was performed on total 155 individuals, including 141 offspring, 8 candidate mothers, 6 fathers. The results real offspring generally agreement with simulation success rate over 99% using only six these loci. allelic diversity observed heterozygosity ( H o )...
The seasonal variations in biochemical composition and reproductive activity of the venus clam Cyclina sinensis (Gmelin) from Yellow River delta China were investigated April 2007 to March 2008 relation environmental factors. According histological observations, gametogenesis began January when water temperature was low. Gametes matured mainly June July condition index (CI) mean oocyte diameter peaked. Spawning occurred primarily August highest, coincided with phytoplankton bloom. CI...
Abstract To evaluate the survival and growth performance of hybrid larvae juveniles, we conducted hybridization experiments between Crassostrea hongkongensis ariakensis at three salinities (15, 23, 30 ppt). Hybrid fertilization occurred in one direction ( C. ♀ ♂), rates did not differ significantly among trial salinities. Molecular genetics analysis revealed that progenies were true hybrids. juveniles survived better than a salinity ppt. showed intermediate progenies. The was greater...
The larval metamorphosis of the veined rapa whelk Rapana venosa was investigated by exposing competent larvae to following seven chemical cues at different concentrations and exposure time: EPI (epinephrine), serotonin, L-DOPA (L-3, 4-dihydroxyphenylalanine), GABA (γ-aminobutyric acid), acetylcholine chloride, KCl (potassium chloride), CaCl2 (calcium chloride). Larvae R. which lost their velum after veliger stage, crawled on foot produced an adult shell were considered have metamorphosed....
The triploid technology is a new frontier in shellfish aquaculture and has shown encouraging results numerous species. We induced larvae the Yesso scallop, Patinopecten yessoensis , using hyperosmotic shock for first time this study. Different induction parameters, including salinity strength, treatment starting point, duration, were tested. highest ratio of D‐shaped (72.12%) was obtained by 60 ppt 20 min at appearance zygote showing polar body II (PB2). A significantly faster growth rate...
We constructed genetic linkage maps of the Japanese scallop Patinopecten yessoensis using AFLP and microsatellite markers. With 32 primer combinations, a total 413 markers (209 from female parent 204 male parent) segregated in 1:1 ratio, corresponding to DNA polymorphisms which were heterozygous one null other. Among six we used, there four polymorphic loci. Two parent, other two both parents. In maternal 161 framework mapped 20 groups, with coverage 2198.8 cM. paternal 166 established map...
Hybridization breeding is deemed to be an effective countermeasure in coping with the genetic decline among marine culture species. Hybrids of Crassostrea angulata ♂ × sikamea ♀ have been shown express heterosis nutritive components and tolerance hypotonic environments. As intertidal zone organisms, they also experience fluctuations water temperature. To better understand cellular molecular processes this hybrid species under cold stress, we analyzed transcriptional response hybrids after 24...