A5079472999- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- Redox biology and oxidative stress
- Nanoparticles: synthesis and applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nanocluster Synthesis and Applications
- Genomics and Phylogenetic Studies
- Mass Spectrometry Techniques and Applications
- Cancer Research and Treatments
Universitat Rovira i Virgili
2015-2017
Broadcom (Spain)
2017
Hospital de Viladecans
2015
Université Paris-Sud
2015
Abstract Recognition of chemical modifications in canonical nucleobases nucleic acids is key importance since such modified variants act as different genetic encoders, introducing variability the biological information contained DNA. Herein, we demonstrate feasibility direct SERS combination with chemometrics and microfluidics for identification relative quantification 4 cytosine both single‐ double‐stranded The minute amount DNA required per measurement, sub‐nanogram regime, removes...
Abstract Point mutations in Ras oncogenes are routinely screened for diagnostics and treatment of tumors (especially colorectal cancer). Here, we develop an optical approach based on direct SERS coupled with chemometrics the study specific conformations that single‐point impose a relatively large fragment K‐Ras gene (141 nucleobases). Results obtained offer unambiguous classification different providing potentially useful insight cancer sensitive, fast, inexpensive manner.
Rapid advances in nanofabrication techniques of reproducibly manufacturing plasmonic substrates with well-defined nanometric scale features and very large electromagnetic enhancements paved the way for final translation analytical potential surface-enhanced Raman scattering (SERS) to real applications. A vast number different SERS have been reported literature. Among others, discrete particles consisting an inorganic micrometric or sub-micrometric core homogeneously coated nanoparticles...
Abstract Recognition of chemical modifications in canonical nucleobases nucleic acids is key importance since such modified variants act as different genetic encoders, introducing variability the biological information contained DNA. Herein, we demonstrate feasibility direct SERS combination with chemometrics and microfluidics for identification relative quantification 4 cytosine both single‐ double‐stranded The minute amount DNA required per measurement, sub‐nanogram regime, removes...
Current understanding of the mechanisms underlying noncovalent interactions between native DNA and nanoparticles, as well their impact on double‐helix structure, is far from providing a comprehensive view. It known that these are largely defined by physicochemical properties metal/liquid interface, in particular nanoparticle surface charge. Remarkably, while unzipping upon binding with cationic nanoparticles reported, exact determinants this structural perturbation remain unclear. Herein,...
Abstract Point mutations in Ras oncogenes are routinely screened for diagnostics and treatment of tumors (especially colorectal cancer). Here, we develop an optical approach based on direct SERS coupled with chemometrics the study specific conformations that single‐point impose a relatively large fragment K‐Ras gene (141 nucleobases). Results obtained offer unambiguous classification different providing potentially useful insight cancer sensitive, fast, inexpensive manner.
Recently, plasmonic-based biosensing has experienced an unprecedented level of attention, with a particular focus on the nucleic acid detection, offering efficient solutions to engineer simple, fast, highly sensitive sensing platforms while overcoming important limitations PCR and microarray techniques. In broad field plasmonics, surface-enhanced Raman scattering (SERS) spectroscopy arisen as powerful analytical tool for detection structural characterization biomolecules. Today applications...
Die Diskriminierung von Einzelpunktmutationen in großen Fragmenten des K-RAS-Gens mittels eines optischen, auf direkter oberflächenverstärkter Raman-Streuung basierenden und mit chemometrischen Methoden gekoppelten Ansatzes wird R. A. Alvarez-Puebla, L. Guerrini et al. der Zuschrift S. 2421 vorgestellt. eindeutige Klassifizierung unterschiedlicher Mutationen liefert einen für die Diagnostik Behandlung Krebs möglicherweise brauchbaren Einblick.
In article number 1700724 Ramon A. Alvarez-Puebla, Luca Guerrini, and co-workers investigate the strand separation of nucleic acid duplexes at junctions cationic nanoparticle clusters by combining plasmon-based spectroscopies theoretical simulations. Geometrical organization size distribution clusters, as well surface charge play major roles in determining extent double-helix dissociation.