Abstract The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine analysis, such as PCR, still suffers from important intrinsic limitations. Surface‐enhanced Raman spectroscopy (SERS) emerged an outstanding opportunity for development but its application to duplexes (dsDNA) been largely hampered reproducibility and/or sensitivity issues. A simple strategy is presented perform ultrasensitive direct label‐free analysis unmodified dsDNA with...

Trace detection of polycyclic aromatic hydrocarbons is reported in this work on dithiocarbamate calix[4]arene functionalized Ag nanoparticles by using surface-enhanced Raman scattering (SERS). SERS spectra informed about the existence pollutant measuring its characteristic fingerprint vibrational features. In addition, revealed important structural information from both host and analyte which was crucial to understand deduce host-guest interaction mechanism. The effectiveness system checked...

The functionalization of silver nanoparticles (Ag NPs) by viologen dications (VGDs) is reported in this work as well their applications the surface-enhanced Raman scattering (SERS) detection polycyclic aromatic hydrocarbons (PAHs). VGDs are able to form intermolecular cavities at interparticle junctions (SERS hot spots) where analyte can be allocated. This leads a giant intensification emission target molecule. effect was applied PAHs, one most widespread and dangerous group pollutants...

Abstract Colorimetric detection of analytes using gold nanoparticles along with surface‐enhanced Raman spectroscopy (SERS) are areas intense research activity since they both offer sensing very low concentrations target species. Multimodal promotes the simultaneous a sample by combination different techniques; consequently, surface chemistry design in development multimodal nanosensors is important for rapid and sensitive evaluation diverse analytical methods. Herein it shown that...

A surface-enhanced Raman scattering (SERS)-based sensor was developed for the detection of oncoprotein c-Jun at nanomolar levels. is a member bZIP (basic zipper) family dimeric transcriptional activators, and its overexpression has been associated with carcinogenic mechanisms in several human cancers. For our sensing purpose, we exploited ability to heterodimerize native protein partner, c-Fos, therefore designed c-Fos peptide receptor chemically modified incorporate thiophenol (TP) group...

Encoded particles are one of the most powerful approaches for multiplex high-throughput screening. Surface-enhanced Raman scattering (SERS) based codification can, in principle, avoid many intrinsic limitations due to conventional alternatives, as it decreases reading time and particle size while allowing almost unlimited codification. Unfortunately, methods synthetic preparation these tedious; often subjected limited reproducibility (associated with large fluctuations distributions polymers...

Chemical speciation and quantification at trace levels of toxic mercury methylmercury ions in water is achieved by SERS spectroscopy.

Protein misfolded proteins are among the most toxic endogenous species of macromolecules. These chemical entities responsible for neurodegenerative disorders such as Alzheimer's, Parkinson's, Creutzfeldt–Jakob's and different non-neurophatic amyloidosis. Notably, these oligomers show a combination marked heterogeneity low abundance in body fluids, which have prevented reliable detection by immunological methods so far. Herein we exploit selectivity to react with metallic ions sensitivity...

Abstract Gold nanostars are among the most efficient plasmonic nanomaterials for optical sensing with surface enhanced Raman scattering. In recent years, gold have increased relevance in physics, analytical chemistry, environmental science, biology and medicine, particular therapy. These colloids can sustain ultrastrong electromagnetic fields which allow their use as single particles enhancers; colloidally stable may a marked catalytical activity. Due to exotic shape, these present...

We report the use of 25,27-diethyl-dithiocarbamic-26,28-dihydroxy-p-tert-butylcalix[4]arene in functionalization Ag nanoparticles for pyrene detection by surface-enhanced Raman scattering (SERS). SERS spectra provided information about calixarene orientation on metal surface and interaction mechanism with pyrene. Thus, this work, we have combined a powerful spectroscopy technique such as SERS, electronic plasmon-based properties nanostructured metals, molecular size-selective recognition...

We report a SERS-based method for the simultaneous and independent determination of two environmental metallic pollutants, Cu(II) Co(II). This was achieved by exploiting coordination-sensitive Raman bands terpyridine (TPY) derivative detecting transition metal ions. Changes in vibrational SERS spectra dithiocarbamate anchored (TPY-DTC) were correlated as function each ion concentration, with limits detection comparable to those several conventional analytical methods. Simultaneous ultratrace...

The standard protocols for DNA analysis largely involve polymerase chain reaction (PCR). However, structures bound to chemical agents cannot be PCR-amplified, and therefore any sequence changes induced by external may neglected. Thus, the development of analytical tools capable characterizing biochemical mechanisms associated with chemically damage is demanded rational design more effective chemotherapy drugs, understanding mode actions carcinogenic chemicals, monitoring genotypic toxicology...

Improved positively charged nanoparticles are described to provide a simplified SERS substrate for DNA detection. Complete flocculation of the is prevented due controlled analyte induced aggregation. This provides stable aggregation state which significantly extends analysis window simplifying detection by SERS.

Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up 70% human cancers, can used as robust proteomic signature cancer. Herein, we developed rapid, highly specific, sensitive surface-enhanced Raman scattering (SERS) assay quantification c-MYC real blood samples. sensing scheme relies on use specifically designed hybrid plasmonic...

Abstract Recognition of chemical modifications in canonical nucleobases nucleic acids is key importance since such modified variants act as different genetic encoders, introducing variability the biological information contained DNA. Herein, we demonstrate feasibility direct SERS combination with chemometrics and microfluidics for identification relative quantification 4 cytosine both single‐ double‐stranded The minute amount DNA required per measurement, sub‐nanogram regime, removes...

Early diagnosis, monitoring and treatment selection of cancer represent major challenges in medicine. The definition the complex clinical molecular landscape requires combination multiple techniques investigation targets. As a result, diagnosis is normally lengthy, expensive and, many cases, cannot be performed recursively. In recent years, optical biosensors, especially those based on unique properties plasmonic nanostructures, have emerged as one most exciting tools nanomedicine, capable...

Abstract The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine analysis, such as PCR, still suffers from important intrinsic limitations. Surface‐enhanced Raman spectroscopy (SERS) emerged an outstanding opportunity for development but its application to duplexes (dsDNA) been largely hampered reproducibility and/or sensitivity issues. A simple strategy is presented perform ultrasensitive direct label‐free analysis unmodified dsDNA with...

As more biological activities of ribonucleic acids continue to emerge, the development efficient analytical tools for RNA identification and characterization is necessary acquire an in-depth understanding their functions chemical properties. Herein, we demonstrate capacity label-free direct surface-enhanced Raman scattering (SERS) analysis access highly specific structural information on RNAs at ultrasensitive level. This includes recognition distinctive vibrational features organized into a...

Herein, we present a fabrication approach that produces homogeneous core–satellite SERS encoded particles with minimal interparticle gaps (<2–3 nm) and maximum particle loading, while positioning the encoding agents at gaps.

Herein, we present an optimized bottom-up approach to fabricate homogeneous Au nanostars with plasmon resonances fully tunable between the red and infrared. The synthetic method relies on kinetic control of reaction upon optimization reactant concentrations (i.e., gold seeds, reducing agent, salt). Optical enhancing properties obtained materials are demonstrated by using SERS visible infrared lasers.