A5062319144- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoparticles: synthesis and applications
- Polymer Surface Interaction Studies
- Nanoparticle-Based Drug Delivery
- Analytical Chemistry and Sensors
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Dendrimers and Hyperbranched Polymers
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- Porphyrin and Phthalocyanine Chemistry
- Caveolin-1 and cellular processes
- Cancer Research and Treatments
- Protein Interaction Studies and Fluorescence Analysis
- RNA Interference and Gene Delivery
- Nanocomposite Films for Food Packaging
- Amino Acid Enzymes and Metabolism
- Quantum Dots Synthesis And Properties
- Advanced Polymer Synthesis and Characterization
- Trypanosoma species research and implications
Universidad Rovira i Virgili
2017-2019
Philipps University of Marburg
2012-2017
Broadcom (Spain)
2016
Abstract The concept of a long‐term sensor for ion changes in the lysosome is presented. made by layer‐by‐layer assembly oppositely charged polyelectrolytes around ion‐sensitive fluorophores, this case protons. spontaneously incorporated cells and resides over days lysosome. Intracellular concentration protons upon cellular stimulation with pH‐active agents are monitored read‐out fluorescence at real time. With help it demonstrated that different used (Monensin, Chloroquine, Bafilomycin A1,...
Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up 70% human cancers, can used as robust proteomic signature cancer. Herein, we developed rapid, highly specific, sensitive surface-enhanced Raman scattering (SERS) assay quantification c-MYC real blood samples. sensing scheme relies on use specifically designed hybrid plasmonic...
In this Progress Report some recent trends and future perspectives towards the use of nanomaterials for smart food packaging quality control are given. The examples discussion meant to illustrate potential nanotechnology sustainability rather than review state art in area, which although expected have a groundbreaking impact on is still an early stage.
The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the also exists particles, and so, its role in particle-cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that proteins adsorbed when exposed blood plasma, forming corona. umbilical vein endothelial cells (HUVECs), was decrease binding...
Colloidal particles with fluorescence read-out are commonly used as sensors for the quantitative determination of ions. Calcium, example, is a biologically highly relevant ion in signaling, and thus knowledge its spatio-temporal distribution inside cells would offer important experimental data. However, use particle-based intracellular detection not straightforward. Important associated problems involve delivery location fluorophores, crosstalk pH, spectral overlap emission spectra different...
Acidification of eukaryotic cell compartments is accomplished by vacuolar H+-ATPases (V-ATPases), large multisubunit complexes able to pump protons into the lumen organelles or in extracellular medium. V-ATPases are involved a number physiological cellular processes, and thus regulation V-ATPase activity crucial importance for cell. Indeed, dysfunction alterations acidification have been recently recognized as key factors variety human diseases. In this study, we applied capsule-based pH...
Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design demonstrate 3D-flow focusing microfluidic device, where all optics are integrated into chip, for fluorescence quantification CTCs in real samples. To test chip performance, two cell membrane targets, epithelial adhesion molecule, EpCAM, receptor tyrosine-protein...
Lysosomal storage disorders are rare genetic diseases characterized by a lysosomal enzyme deficiency. The defect leads to an accumulation of normally degraded substrates within the lysosomes. polymeric capsules inside lysosomes is exploited create universal in vitro theranostic tool for disorders. diagnostic ability this based on pH-sensitive fluorophores demonstrated monitoring pH Krabbe-disease cell models upon substrate psychosine. Krabbe-affected cells maintain their normal pH, while...
Quantification of circulating tumor cells (CTCs) in blood samples from cancer patients is a non-invasive approach to monitoring the status disease. Most methods proposed recent years are phenomenological and rely on use antibodies labelled with fluorophores, magnetic particles, or immobilized surfaces capture CTCs. Herein, we designed optimized method that employs glucose analogue fluorophore which takes advantage different metabolic pathways discern them normal ones. Notably, demonstrate...
Polymer multilayer capsules are used by P. Rivera_Gil, W. J. Parak, and co-workers for concomitant pH-sensing enzyme delivery to lysosomes in the framework of enzyme-replacement therapy lysosomal-storage disorders, as described on page 991. The act local reporters pH changing fluorescence carried sensor from red (alkaline) yellow (acidic) upon endocytosis organelle acidification. In acidic lysosomes, deliver substitute defective one.