Inhibitors of DNA-binding proteins (Id1-4), lacking the basic domain, function as dominant inhibitors cell-cycle regulators. Overexpression Id promotes cancer cell proliferation and resistance against apoptosis. Level protein expression, especially Id1, correlates with poor differentiation, enhanced malignant potential more aggressive clinical behaviour ovarian tumours. Although overexpression Ids has been found shown to correlate outcome, their inhibition at level never studied.A peptide...

Annulus fibrosus repair techniques for the intervertebral disc (IVD) address unsolved problem of reherniation after IVD herniation and might facilitate development nucleus pulposus replacement diseases. This study investigates suitability a bio-integrative annulus implant.Standardized box defects were applied to L3/4 L4/5 16 sheep, followed by randomized insertion textile polyglycolic acid/polyvinylidene fluoride implant in one defects. Explantation was conducted 2, 6 12 weeks, provocative...

In addition to the known response regulator ErbR (former AgmR) and two-component regulatory system EraSR ExaDE), three additional proteins have been identified as being involved in controlling transcription of aerobic ethanol oxidation Pseudomonas aeruginosa. Two putative sensor kinases, ErcS ErcS', a regulator, ErdR, were found, all which show significant similarity flhSR that controls methanol formaldehyde metabolism Paracoccus denitrificans. All regulators, EraR (formerly ExaE), are...

Painful degenerative disc diseases have been targeted by different biological treatment approaches. Nucleus pulposus (NP) cells play a central role in intervertebral (IVD) maintenance orchestrating catabolic, anabolic and inflammatory factors that affect the extracellular matrix. IVD degeneration is associated with imbalances of these factors, resulting catabolic metabolism. Therefore, accurate knowledge about their quantity quality regard to matrix synthesis vital for rational gene...

Several two-component regulatory systems are known to be involved in the signal transduction pathway of ethanol oxidation system Pseudomonas aeruginosa ATCC 17933. These sensor kinases and response regulators organized a hierarchical manner. In addition, cytoplasmic putative iron-containing alcohol dehydrogenase (Fe-ADH) encoded by ercA (PA1991) has been identified play an essential role this network. The gene is located next ercS, which encodes kinase. Inactivation insertion kanamycin...

Degenerative disc disease (DDD) of the cervical spine is common after middle age and can cause loss height with painful nerve impingement, bone joint inflammation. Despite clinical importance these problems, in current publications pathology degeneration has been studied merely from a morphologic view point using magnetic resonance imaging (MRI), without addressing issue biological treatment approaches. So far wide range endogenously expressed bioactive factors degenerative cells not yet...

Intervertebral disc (IVD) degeneration that accelerates the loss of structural and functional integrities is recognized as one major factors chronic back pain. Cardiovascular risk factors, such deficits apolipoproteins elevate levels cholesterol triglycerides, are considered critical for progress atherosclerosis; notably in abdominal aorta its lumbar branching arteries supply vertebrae IVDs. Obstruction by atherosclerosis presumed to promote low APOE-knockout rabbits have recently been shown...

Abstract Background ID proteins are dominant negative inhibitors of basic helix-loop-helix transcription factors that have multiple functions during development and cellular differentiation. Ectopic (over-)expression ID1 extends the lifespan primary human epithelial cells. High expression levels been detected in malignancies, some correlated with unfavorable clinical prognosis. protein is localized at centrosomes forced results errors centrosome duplication. Results Here we analyzed steady...

Inhibition of intervertebral disc (IVD) degeneration, which is often accompanied by painful inflammatory and immunopathological processes, challenging. Current IVD gene therapeutic approaches are based on adenoviral delivery systems, limited immune reactions to their viral proteins. Their applications in IVDs near sensitive neural structure could provoke toxicity immunological side-effects with neurological deficits. Self-complementary adeno-associated virus (scAAV) vectors, do not express...

Intervertebral disc (IVD) disorders are often accompanied by painful inflammatory and immunopathological processes. Nucleus pulposus (NP) cells play a pivotal role in maintenance of IVD organizing the expression anabolic, catabolic, anti-catabolic cytokines. Human NP have been targeted gene therapeutic approaches using lentiviral or adenoviral systems that could be critical due to genome incorporation immunological side effects. Adeno-associated viruses (AAVs), which do not express any viral...

Rabbits with naturally high levels of cholesterol ester transfer protein (CETP), unlike rodents, have become an interesting animal model for the study lipid metabolism and atherosclerosis, as they similarities to humans in metabolism, cardiovascular physiology susceptibility develop atherosclerosis. Rodents, such mice, are not prone atherosclerosis lack mass activity CETP, a key player lipoprotein metabolism. Recently, APOE-knockout rabbits has been shown promote associated premature IVD...

Green tea is associated with decreased risk for cardiovascular disease and stroke. Matcha a special kind of powdered green known its use in the Japanese ceremony. Due to influence on lipoprotein parameters, it has been postulated exert antiatherogenic effects. This study investigates whether modulates high-density (HDL) function thereby influences atherogenic process an animal model strong humans' situation.

Intervertebral disc (IVD) degeneration damaging the extracellular matrix (ECM) of IVDs is main cause spine-associated disorders. Degenerative disease (DDD) a multifaceted disorder, where environmental factors, inflammatory cytokines and catabolic enzymes act together. DDD starts typically due to imbalance between ECM biosynthesis degradation within IVDs, especially through unbalanced aggrecan collagen II in nucleus pulposus (NP). Current treatment approaches are primarily based on...

Degenerative disk disease (DDD) that aggravates structural deterioration of intervertebral disks (IVDs) can be accompanied by painful inflammation and immunopathological progressions. Current surgical or pharmacological therapies cannot repair the structure function IVDs. Nucleus pulposus (NP) cells are crucial for preservation restoration IVDs balancing anabolic catabolic factors affecting extracellular matrix. Imbalanced cause increased degradation aggrecan. Aggrecanases A Disintegrin And...

Introduction Painful degenerative disc diseases have been targeted by different biological treatment approaches. Nucleus pulposus (NP) cells play a central role in intervertebral (IVD) maintenance orchestrating catabolic, anabolic, and inflammatory factors that affect the extracellular matrix. IVD degeneration is associated with imbalances of these factors, resulting catabolic metabolism. Therefore, accurate knowledge about their quantity quality regard to matrix synthesis vital for rational...