A5049428782- Luminescence and Fluorescent Materials
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Nanoplatforms for cancer theranostics
- Click Chemistry and Applications
- Enzyme Production and Characterization
- Food composition and properties
- Advanced Fluorescence Microscopy Techniques
- Amino Acid Enzymes and Metabolism
Michigan State University
2022-2024
Human cellular retinol binding protein II (hCRBPII) was used as a engineering platform to rationally regulate absorptive and emissive properties of covalently bound fluorogenic dye. We demonstrate the thio-dapoxyl analog via formation protonated imine between an active site lysine residue chromophore's aldehyde. Rational manipulation electrostatics pocket results in 204 nm shift absorption 131 emission. The is readily expressed mammalian systems binds with exogenously delivered fluorophore...
Glycogen is the primary storage polysaccharide in bacteria and animals. It a glucose polymer linked by α-1,4 linkages branched via α-1,6-linkages, with latter reaction catalyzed branching enzymes. Both length dispensation of these branches are critical defining structure, density, relative bioavailability polysaccharide. Key to this specificity enzymes because they define branch length. Herein, we report crystal structure maltooctaose-bound enzyme from enterobacteria E. coli. The identifies...
An engineered hCRBPII protein binds a coumarin-based fluorophore to yield photoswitchable system via Michael/retro-Michael addition of cysteine residue. Alternate UV/visible light irradiation accesses the two optical states.